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Golkar, S., Chekini, Z., Amjadi, F., Afsharian, P., Najafian, A., Ghaffari, F., & Aflatoonian, R. (2023). Expressions of vascular endothelial growth factor A, mucin-1, colony-stimulating factor-1, heparinbinding epidermal growth factor-like growth factor, and fibroblast growth factor 2 genes in the female reproductive tracts of women with ectopic pregnancy. International Journal of Reproductive BioMedicine (IJRM), 21(10), 801–808. https://doi.org/10.18502/ijrm.v21i10.14535

https://doi.org/10.18502/ijrm.v21i10.14535

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authors

  • Sima Golkar

    Sima Golkar

    Department of Genetics Science, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, ACECR, Tehran, Iran.
  • Zahra Chekini

    Zahra Chekini

    Department of Endocrinology and Female Infertility, Reproductive Biomedicine Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
  • Fatemehsadat Amjadi

    Fatemehsadat Amjadi

    Department of Anatomy, School of Medicine, Iran University of Medical Science, Tehran, Iran.
  • Parvaneh Afsharian

    Parvaneh Afsharian

    Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
  • Aida Najafian

    Aida Najafian

    Department of Infertility, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
  • Firouzeh Ghaffari

    Firouzeh Ghaffari

    Department of Endocrinology and Female Infertility, Reproductive Biomedicine Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
  • Reza Aflatoonian

    Reza Aflatoonian

    Department of Endocrinology and Female Infertility, Reproductive Biomedicine Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Published Date

  • Nov 21, 2023

Expressions of vascular endothelial growth factor A, mucin-1, colony-stimulating factor-1, heparinbinding epidermal growth factor-like growth factor, and fibroblast growth factor 2 genes in the female reproductive tracts of women with ectopic pregnancy

International Journal of Reproductive BioMedicine (IJRM) / International Journal of Reproductive BioMedicine (IJRM): Volume 21, Issue No. 10 / Pages 801–808

Abstract

Full Title: Expressions of vascular endothelial growth factor A, mucin-1, colony-stimulating factor-1, heparin-binding epidermal growth factor-like growth factor, and fibroblast growth factor 2 genes in the female reproductive tracts of women with ectopic pregnancy: A case-control study


Background: Ectopic pregnancy (EP) is defined as embryo implantation in a location other than the uterine cavity.


Objective: We aimed to evaluate the expression of several genes, which may play a role in EP, in the ampulla region of fallopian tubes and endometrial tissue of women with EP.


Materials and Methods: In this case-control study, 5 women who underwent salpingectomy due to EP, comprised the 5 pseudo-pregnant women as a control group. These participants referred to the Royan Institute, Shariati, and Arash hospital, Tehran, Iran during 2019-2021. We evaluated the expressions of vascular endothelial growth factor A, mucin-1, colony-stimulating factor-1, heparin-binding epidermal growth factor-like growth factor (HBEGF), and fibroblast growth factor 2 genes in the fallopian tube and endometrium of EP cases by real-time polymerase chain reaction using specific primers.


Results: The vascular endothelial growth factor expression was significantly higher in the ampulla region of the controls. However, no significant differences were observed in endometrial tissue. Assessments of colony-stimulating factor-1 and fibroblast growth factor 2 showed no significant differences between the case and control groups. HBEGF showed significantly higher expression in the ampulla region of EP cases, but no significant difference was observed in HBEGF expression in the endometrial tissues of the study groups. Mucin-1 expression was significantly higher in both study regions of the EP cases.


Conclusion: Our results have strongly suggested that these genes play important roles in proper implantation, and disruptions in their expression patterns could lead to EP. However, more studies are needed to confirm the current findings.


Key words: Ectopic pregnancy, Gene expression, Vascular endothelial growth factor A, Mucin-1, Colony-stimulating factor-1, Heparin-binding epidermal growth factor-like growth factor, Fibroblast growth factor 2.

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