https://doi.org/10.18502/ijrm.v21i3.13199
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authors
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Raphael Eguono Uwejigho
Raphael Eguono Uwejigho
Department of Anatomy, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Laje Campus, Ondo City, Ondo State, Nigeria.
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Kingsley Afoke Iteire
Kingsley Afoke Iteire
Department of Anatomy, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Laje Campus, Ondo City, Ondo State, Nigeria.
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Felix Udawmojo Enemali
Felix Udawmojo Enemali
Department of Anatomy, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo State, Laje Campus, Ondo City, Ondo State, Nigeria.
Published Date
- Apr 11, 2023
Anti-fertility effect of Aerva lanata crude extract in male Dams offspring: An experimental study
Abstract
Background: Aerva lanata, a herb used as food and also consumed as a tonic by pregnant women to relieve stomach pains and prevent miscarriage. In addition to other characterized properties, it possesses antifertility and anti-implantation activities.
Objective: This study investigates the testicular toxicity of the testes of offsprings of Dams treated with crude aqueous extract of Aerva lanata.
Materials and Methods: 25 pregnant Wistar rats (Dams) weighing 180-240 gr were randomly earmarked into 5 groups (n = 5/each). Group A served as control; groups B, C, D, and E received 200, 400, 800, and 1000 mg/kg body weight of Aerva lanata extract, respectively, beginning from 12th to 19th day of gestation. The pups (delivered of Dams) were weighed, observed, and sacrificed 6 wk post-parturition. The testes of the male pups were obtained for histological procedures the testis histology was examined.
Results: No gross malformation was observed in the treatment groups, the number of pups/litter was significantly reduced in group E (p = 0.01), pups weight analysis showed a significant reduction in groups C and E (p = 0.04, and 0.02 respectively), and the mean pup testes weight was significantly reduced in groups B, C, D, and E (p = 0.03, 0.03, 0.01, and < 0.001 respectively) when compared with control. Histologically, the treated pup testes tissues showed varying degrees of disruption and distortion of the cellular arrangements of the germinal epithelium in a dose-dependent manner compared to the control.
Conclusion: The study revealed a testicular toxicity and possibly antifertility role of Aerva lanata in dams’ pups.
Key words: Amaranthaceae, Fertility, Male fertility, Male reproductive system, Local herbs.
References
[2] Mintah SO, Asafo-Agyei T, Archer MA, Atta-Adjei Junior P, Boamah D, Kumadoh D, et al. Medicinal plants for treatment of prevalent diseases. In: Perveen Sh, Al-Taweel A. Pharmacognosy: Medicinal plants. UK: Intechopen; 2018.
[3] Akunne TC, Uzor Ph, Onyeto CA, Okereke EK. Safe African Medicinal plants for clinical studies. In: Kuete V. Toxicological survey of African Medicinal plants. London: Elsevier; 2014: 535–555.
[4] Karemore MN, Avari JG. Herbal medicines used during pregnancy, childbirth and postpartum care. Int J Pharm Sci Res 2017; 8: 5326–5335.
[5] Musaddiqa S, Mustafaa K, Ahmadb S, Aslama S, Alic B, Khakwania S, et al. Pharmaceutical, ethnopharmacological, phytochemical and synthetic importance of genus Aerva: A review. Natural Product Communications 2018; 13: 375–385.
[6] Zhao Y, Kumar D, Prasad DN, Singhb RK, Ma Y. Morphoanatomic, physicochemical, and phytochemical standardization with HPTLC fingerprinting of aerial parts of Aerva lanata (Linn) Juss ex schult. J Trad Chinese Med Sci 2015; 2: 39–44.
[7] Savadi RV, Alagawadi KR. Antifertility activity of ethanolic extracts of plumbago indica and Aerva lanata on albino rats. Int J Green Pharmcol 2009; 3: 230–233.
[8] Carlson B. Human embryology and developmental biology. 5th Ed. Philadelphia: Saunders; 2013.
[9] Costa KCS, Bezerra SB, Norte CM, Nunes LMN, de Olinda TM. Medicinal plants with teratogenic potential: Current considerations. Braz J Pharm Sci 2012; 48: 427–433.
[10] Ghuman S, Coopoosamy RM. Crude sample preparation, extraction and in vitro screening for antimicrobial activity of selected wound healing medicinal plants in KwaZulu- Natal, South Africa: A review. J Med Plant Res 2011; 5: 3572–3576.
[11] Kpemissi M, Eklu-Gadegbeku K, Veerapur VP, Potarniche AV, Adi K, Vijayakumar S, et al. Antioxidant and nephroprotection activities of Combretum micranthum: A phytochemical, in-vitro and ex-vivo studies. Heliyon 2019; 5: e01365.
[12] Kpemissi M, Eklu-Gadegbeku K, Veerapur VP, Negru M, Taulescu M, Chandramohan V, et al. Nephroprotective activity of Combretum micranthum G. Don in cisplatin induced nephrotoxicity in rats: In-vitro, in-vivo and in-silico experiments. Biomed Pharmacother 2019; 116: 108961.
[13] Ema M, Miyawaki E, Kawashima K. Critical period for adverse effects on development of reproductive system in male offspring of rats given di-n-butyl phthalate during late pregnancy. Toxicol Lett 2000; 111: 271–278.
[14] Picut CA, Remick AK, de Rijk EP, Simons ML, Stump DG, Parker GA. Postnatal development of the testis in the rat: Morphologic study and correlation of morphology to neuroendocrine parameters. Toxicol Pathol 2015; 43: 326–342.
[15] Shields VDC, Heinbockel Th. Introductory chapter: Histological microtechniques. In: Heinbockel Th, Shields VDC. Histology. UK: IntechOpen; 2018.
[16] Ankul SS, Gowri K, Chitra V. A review on phytochemical constituents and pharmacological activities of the plant: Aerva Lanata. Res J Pharm Technol 2020; 13: 1580–1586.
[17] Weichbrod RH, Thompson GAH, Norton JN. Management of animal care and use programs in research, education, and testing. 2nd Ed. Boca Raton (FL): CRC Press/Taylor & Francis; 2018.
[18] Athira P, Nair SN. Pharmacognostic review of medicinal plant Aerva lanata. J Pharn Sci Res 2017; 9: 1420–1423.
[19] Omotoso KS, Aigbe FR, Salako OA, Chijioke MC, Adeyemi OO. Toxicological evaluation of the aqueous whole plant extracts of Aerva lanata Juss. ex Schult (Amaranthaceae). J Ethnopharmacol 2017; 208: 174–184.
[20] Bitasta M, Madan S. Aerva lanata: A blessing of mother nature. J Pharmacogn Phytochem 2016; 5: 92–101.
[21] Shaik A, Yalavarthi PR, Bannoth CK. Role of anti-fertility medicinal plants on male & female reproduction. J Complement Altern Med Res 2017; 3: 1–22.
[22] Daniyal M, Akram M. Antifertility activity of medicinal plants. J Chinese Med Assoc 2015; 78: 382–388.
[23] Oduwole OO, Peltoketo H, Huhtaniemi IT. Role of follicle-stimulating hormone in spermatogenesis. Front Endocrinol 2018; 9: 763.
[24] O’Shaughnessy PJ, Fowler PA. Endocrinology of the mammalian fetal testis. Reproduction 2011; 141: 37–46.
[25] D’Cruz SC, Vaithinathan S, Jubendradass R, Mathur PP. Effects of plants and plant products on testis. Asian J Androl 2010; 12: 468–479.
[26] Dewal S, Sharma R, Gupta RS. A review on antifertility activities of plants of Chambal riverine. Asian J Pharm Clini Res 2018; 11: 8–14.