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Fattahi, M., Maghsudlu, M., & Sheikhha, M. H. (2023). Is sperm telomere length altered in teratozoospermia specimens? A case-control study. International Journal of Reproductive BioMedicine (IJRM), 21(3), 229–236. https://doi.org/10.18502/ijrm.v21i3.13198
https://doi.org/10.18502/ijrm.v21i3.13198
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authors
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Maryam Fattahi
Maryam Fattahi
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
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Mohadese Maghsudlu
Mohadese Maghsudlu
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
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Mohammad Hasan Sheikhha
Mohammad Hasan Sheikhha
Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Published Date
- Apr 11, 2023
Is sperm telomere length altered in teratozoospermia specimens? A case-control study
Abstract
Background: Male factor infertility is a multifactorial defect, and many of its etiologies are unknown. Teratozoospermia is determined by the existence of over 85% morphologically abnormal spermatozoa in semen which are almost incompetent in fertilization function. One of the most novel issues in genetic alterations studies is the variation of sperm telomere lengths (STL) and its collaboration with male infertility. The present study has been focused on STL alterations in teratozoospermia.
Objective: Investigation of differences in telomere length of teratozoospermia specimens and sperms with normal parameters.
Materials and Methods: In this case-control study, 60 men referred to Arak Fertility Clinic, Markazi province, Iran from November 2017 to February 2018 were categorized into teratozoospermia and normozoospermic groups. Sperm genomic DNA extraction was conducted, and STL were evaluated using quantitative polymerase chain reaction.
Results: Statistical evaluation of relative telomere length was calculated by the ratio of telomere to single-copy gene for teratozoospermia and normal specimens. Results significantly demonstrated that relative telomere length in teratozoospermia samples is nearly 3 times shorter than in normal samples (p > 0.001).
Conclusion: Our results represent the reduction of telomeres length in teratozoospermia and suggest that this alteration might be one of the factors contributing to the sperm fertility potential of this kind of specimen. However, defining relevant molecular processes requires further detailed investigations.
Key words: Telomere, Teratozoospermia, Sperm, Male infertility.
References
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[19] Ferlin A, Rampazzo E, Rocca MS, Keppel S, Frigo AC, De Rossi A, et al. In young men sperm telomere length is related to sperm number and parental age. Hum Reprod 2013; 28: 3370–3376.
[20] Mishra SS, Kumar S, Singh G, Mohanty K, Vaid S, Malhotra N, et al. Oxidative DNA damage in male germ cells in normozoospermic infertile men: A case for concern. Austin J Reprod Med Infertil 2015; 2: 1017.
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[23] Liu SY, Zhang C-J, Peng H-Y, Huang X-Q, Sun H, Lin K-Q, et al. [Association study of telomere length with idiopathic male infertility]. Yi Chuan 2015; 37: 1137–1142. (in Chinese)
[24] Biron-Shental T, Wiser A, Hershko-Klement A, Markovitch O, Amiel A, Berkovitch A. Sub-fertile sperm cells exemplify telomere dysfunction. J Assist Reprod Genet 2018; 35: 143–148.
[25] Kurjanowicz P. Evaluation of telomere length in spermatozoa as a diagnostic tool for male factor infertility [Ph.D. thesis]. Toronto: University of Toronto (Canada) ProQuest Dissertations Publishing; 2022.
[26] Vyas N, Gamit K, Raval M. Male infertility: A major problem worldwide and its management in ayurveda. Pharma Science Monitor 2018; 9: 446–469.
[27] Practice Committee of the American Society for Reproductive Medicine. Diagnostic evaluation of the infertile female: A committee opinion. Fertil Steril 2015; 103: e44–e50.
[28] Edition F. Examination and processing of human semen. Geneva: World Health Organization Press; 2010.
[29] Agarwal A, Tvrda E, Sharma R. Relationship amongst teratozoospermia, seminal oxidative stress and male infertility. Reprod Biol Endocrinol 2014; 12: 45.
[30] Krausz C, Riera-Escamilla A. Genetics of male infertility. Nature Reviews Urology 2018; 15: 369–384.
[31] Podlevsky JD, Chen JJ-L. It all comes together at the ends: Telomerase structure, function, and biogenesis. Mutat Res 2012; 730: 3–11.
[32] Reig-Viader R, Garcia-Caldés M, Ruiz-Herrera A. Telomere homeostasis in mammalian germ cells: A review. Chromosoma 2016; 125: 337–351.
[33] Balmori C, Varela E. Should we consider telomere length and telomerase activity in male factor infertility? Curr Opin Obstet Gynecol 2018; 30: 197–202.
[34] Jorgensen PB, Fedder J, Koelvraa S, Graakjaer J. Agedependence of relative telomere length profiles during spermatogenesis in man. Maturitas 2013; 75: 380–385.
[35] Long J, Huang C, Chen Y, Zhang Y, Shi S, Wu L, et al. Telomeric TERB1–TRF1 interaction is crucial for male meiosis. Nat Struct Mol Biol 2017; 24: 1073–1080.
[36] De Frutos C, López-Cardona AP, Balvís NF, Laguna-Barraza R, Rizos D, Gutierrez-Adán A, et al. Spermatozoa telomeres determine telomere length in early embryos and offspring. Reproduction 2016; 151: 1–7.
[37] Fice HE, Robaire B. Telomere dynamics throughout spermatogenesis. Genes 2019; 10: 525.
[2] Agarwal A, Baskaran S, Parekh N, Cho C-L, Henkel R, Vij S, et al. Male infertility. Lancet 2021; 397: 319–333.
[3] Leaver RB. Male infertility: An overview of causes and treatment options. Br J Nurs 2016; 25: S35–S40.
[4] De Braekeleer M, Nguyen MH, Morel F, Perrin A. Genetic aspects of monomorphic teratozoospermia: A review. J Assist Reprod Genet 2015; 32: 615–623.
[5] Ammar O, Mehdi M, Muratori M. Teratozoospermia: Its association with sperm DNA defects, apoptotic alterations, and oxidative stress. Andrology 2020; 8: 1095–1106.
[6] Beurois J, Cazin C, Kherraf Z-E, Martinez G, Celse T, Toure A, et al. Genetics of teratozoospermia: Back to the head. Best Pract Res Clin Endocrinol Metab 2020; 34: 101473.
[7] Kim HJ, Yoon HJ, Jang JM, Oh HS, Lee YJ, Lee WD, et al. Comparison between intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection in oligo-astheno-teratozoospermia patients. Clin Exp Reprod Med 2014; 41: 9–14.
[8] Ozturk S. Telomerase activity and telomere length in male germ cells. Biol Reprod 2015; 92: 53.
[9] Miyamoto T, Minase G, Shin T, Ueda H, Okada H, Sengoku K. Human male infertility and its genetic causes. Reprod Med Biol 2017; 16: 81–88.
[10] Lu W, Zhang Y, Liu D, Songyang Z, Wan M. Telomeresstructure, function, and regulation. Exp Cell Res 2013; 319: 133–141.
[11] Shay JW, Wright WE. Telomeres and telomerase: Three decades of progress. Nat Rev Genet 2019; 20: 299– 309.
[12] Darmishonnejad Z, Tavalaee M, Izadi T, Tanhaei S, Nasr-Esfahani MH. Evaluation of sperm telomere length in infertile men with failed/low fertilization after intracytoplasmic sperm injection. Reprod Biomed Online 2019; 38: 579–587.
[13] Kalmbach KH, Antunes DMF, Dracxler RC, Knier TW, Seth-Smith ML, Wang F, et al. Telomeres and human reproduction. Fertil Steril 2013; 99: 23–29.
[14] Rocca MS, Foresta C, Ferlin A. Telomere length: Lights and shadows on their role in human reproduction. Biol Reprod 2019; 100: 305–317.
[15] Boniewska-Bernacka E, Panczyszyn A, Cybulska N. Telomeres as a molecular marker of male infertility. Hum Fertil 2019; 22: 78–87.
[16] Thilagavathi J, Kumar M, Mishra S, Venkatesh S, Kumar R, Dada R. Analysis of sperm telomere length in men with idiopathic infertility. Arch Gynecol Obstet 2013; 287: 803–807.
[17] Cawthon RM. Telomere measurement by quantitative PCR. Nucleic Acids Res 2002; 30: e47.
[18] Boniewska-Bernacka E, Pańczyszyn A, Cybulska N. Sperm telomere length in men with normal and abnormal semen parameters. A pilot study. Biomed J Sci Tech Res 2019; 21: 15639–15645.
[19] Ferlin A, Rampazzo E, Rocca MS, Keppel S, Frigo AC, De Rossi A, et al. In young men sperm telomere length is related to sperm number and parental age. Hum Reprod 2013; 28: 3370–3376.
[20] Mishra SS, Kumar S, Singh G, Mohanty K, Vaid S, Malhotra N, et al. Oxidative DNA damage in male germ cells in normozoospermic infertile men: A case for concern. Austin J Reprod Med Infertil 2015; 2: 1017.
[21] Cariati F, Jaroudi S, Alfarawati S, Raberi A, Alviggi C, Pivonello R, et al. Investigation of sperm telomere length as a potential marker of paternal genome integrity and semen quality. Reprod Biomed Online 2016; 33: 404–411.
[22] Berneau SC, Shackleton J, Nevin C, Altakroni B, Papadopoulos G, Horne G, et al. Associations of sperm telomere length with semen parameters, clinical outcomes and lifestyle factors in human normozoospermic samples. Andrology 2020; 8: 583–593.
[23] Liu SY, Zhang C-J, Peng H-Y, Huang X-Q, Sun H, Lin K-Q, et al. [Association study of telomere length with idiopathic male infertility]. Yi Chuan 2015; 37: 1137–1142. (in Chinese)
[24] Biron-Shental T, Wiser A, Hershko-Klement A, Markovitch O, Amiel A, Berkovitch A. Sub-fertile sperm cells exemplify telomere dysfunction. J Assist Reprod Genet 2018; 35: 143–148.
[25] Kurjanowicz P. Evaluation of telomere length in spermatozoa as a diagnostic tool for male factor infertility [Ph.D. thesis]. Toronto: University of Toronto (Canada) ProQuest Dissertations Publishing; 2022.
[26] Vyas N, Gamit K, Raval M. Male infertility: A major problem worldwide and its management in ayurveda. Pharma Science Monitor 2018; 9: 446–469.
[27] Practice Committee of the American Society for Reproductive Medicine. Diagnostic evaluation of the infertile female: A committee opinion. Fertil Steril 2015; 103: e44–e50.
[28] Edition F. Examination and processing of human semen. Geneva: World Health Organization Press; 2010.
[29] Agarwal A, Tvrda E, Sharma R. Relationship amongst teratozoospermia, seminal oxidative stress and male infertility. Reprod Biol Endocrinol 2014; 12: 45.
[30] Krausz C, Riera-Escamilla A. Genetics of male infertility. Nature Reviews Urology 2018; 15: 369–384.
[31] Podlevsky JD, Chen JJ-L. It all comes together at the ends: Telomerase structure, function, and biogenesis. Mutat Res 2012; 730: 3–11.
[32] Reig-Viader R, Garcia-Caldés M, Ruiz-Herrera A. Telomere homeostasis in mammalian germ cells: A review. Chromosoma 2016; 125: 337–351.
[33] Balmori C, Varela E. Should we consider telomere length and telomerase activity in male factor infertility? Curr Opin Obstet Gynecol 2018; 30: 197–202.
[34] Jorgensen PB, Fedder J, Koelvraa S, Graakjaer J. Agedependence of relative telomere length profiles during spermatogenesis in man. Maturitas 2013; 75: 380–385.
[35] Long J, Huang C, Chen Y, Zhang Y, Shi S, Wu L, et al. Telomeric TERB1–TRF1 interaction is crucial for male meiosis. Nat Struct Mol Biol 2017; 24: 1073–1080.
[36] De Frutos C, López-Cardona AP, Balvís NF, Laguna-Barraza R, Rizos D, Gutierrez-Adán A, et al. Spermatozoa telomeres determine telomere length in early embryos and offspring. Reproduction 2016; 151: 1–7.
[37] Fice HE, Robaire B. Telomere dynamics throughout spermatogenesis. Genes 2019; 10: 525.