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Javanmard, F., Bidmeshkipour, A., & Ghasemian Yadegari, J. (2023). Ceratonia siliqua L improved cryodamage of asthenozoospermic specimens: An experimental study. International Journal of Reproductive BioMedicine (IJRM), 20(12), 1029–1038. https://doi.org/10.18502/ijrm.v20i12.12564
https://doi.org/10.18502/ijrm.v20i12.12564
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Fatemeh Javanmard
Fatemeh Javanmard
Department of Biology, Faculty of Basic Science, Razi University, Kermanshah, Iran.
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Ali Bidmeshkipour
Ali Bidmeshkipour
Department of Biology, Faculty of Basic Science, Razi University, Kermanshah, Iran.
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Javad Ghasemian Yadegari
Javad Ghasemian Yadegari
Department of Pharmacognosy, School of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.
Published Date
- Jan 9, 2023
Ceratonia siliqua L improved cryodamage of asthenozoospermic specimens: An experimental study
Abstract
Background: Sperm freezing is an important procedure in assisted reproductive technology. Freezing results in physical and chemical changes in the sperm. Ceratonia siliqua L (C.siliqua) is a tree that has antioxidant properties.
Objective: This study aimed to investigate the effect of different concentrations of C.siliqua in a freezing medium on semen parameters, and some biochemical parameters in asthenozoospermic specimens.
Materials and Methods: Forty asthenozoospermic specimens (semen specimens with motility < 32%) were obtained from men aged between 20-40 yr according to the World Health Organization criteria. Each sample was divided into 6 groups: I) fresh, II) control, III) 5, IV) 10, V) 20, and VI) 30 μg/ml C.siliqua extract were added to a freezing medium respectively. Then sperm parameters, malondialdehyde, total antioxidant capacity, reactive oxygen species, and sperm DNA assay were evaluated using related protocols after thawing.
Results: Data analysis shows that sperm parameter, and total antioxidant capacity level increased at a concentration of 20 μg/ml of C. siliqua extract compared to the other concentrations of C.siliqua extract after cryopreservation and thawing (p < 0.001). Also, the sperm DNA fragmentation assay, reactive oxygen species, and malondialdehyde levels were significantly reduced by adding 20 μg/ml of C. siliqua extract to the sperm freezing medium compared to the other treated groups after cryopreservation (p < 0.001).
Conclusion: C.siliqua extract significantly improved sperm parameters after cryopreservation and thawing in asthenozoospermic specimens, and the greatest impact was observed at the 20 μg/ml C.siliqua L extract concentration (p < 0.001).
Key words: Asthenozoospermia, Ceratonia, Cryopreservation, Fertility preservation, Infertility, Male.
References
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[2] Tao Y, Sanger E, Saewu A, Leveille M-C. Human sperm vitrification: The state of the art. Reprod Biol Endocrinol 2020; 18: 17.
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[4] Le MT, Nguyen TTT, Nguyen TT, Nguyen VT, Nguyen TTA, Nguyen VQH, et al. Cryopreservation of human spermatozoa by vitrification versus conventional rapid freezing: Effects on motility, viability, morphology and cellular defects. Eur J Obstet Gynecol Reprod Biol 2019; 234: 14-20.
[5] Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology 2016; 85: 47-64.
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[7] Subramanian V, Ravichandran A, Thiagarajan N, Govindarajan M, Dhandayuthapani S, Suresh S. Seminal reactive oxygen species and total antioxidant capacity: Correlations with sperm parameters and impact on male infertility. Clin Exp Reprod Med 2018; 45: 88-93.
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[11] Berkovitz A, Allouche-Fitoussi D, Izhakov D, Breitbart H. Cryopreservation of human sperm in the presence of Zn2+ increases the motility rate. J Obstet Gynecol Investig 2018; 1: e6-e12.
[12] Truong TT, Gardner DK. Antioxidants increase blastocyst cryosurvival and viability post-vitrification. Hum Reprod 2020; 35: 12-23.
[13] Iovine C, Mottola F, Santonastaso M, Finelli R, Agarwal A, Rocco L. In vitro ameliorative effects of ellagic acid on vitality, motility and DNA quality in human spermatozoa. Mol Reprod Dev 2021; 88: 167-174.
[14] Santonastaso M, Mottola F, Iovine C, Colacurci N, Rocco L. Protective effects of curcumin on the outcome of cryopreservation in human sperm. Reprod Sci 2021; 28: 2895-2905.
[15] Faramarzi A, Aghaz F, Golestan Jahromi M, Bakhtiari M, Khazaei M. Does supplementation of sperm freezing/thawing media with Ceratonia siliqua improve detrimental effect of cryopreservation on sperm parameters and chromatin quality in normozoospermic specimens? Cell Tissue Bank 2019; 20: 403-409.
[16] Rtibi K, Jabri MA, Selmi S, Souli A, Sebai H, El-Benna J, et al. Gastroprotective effect of carob (Ceratonia siliqua L.) against ethanol-induced oxidative stress in rat. BMC Complement Altern Med 2015; 15: 292.
[17] Vafaei A, Mohammadi Sh, Fazel A, Soukhtanloo M, Mohammadipour A, Beheshti F. Effects of carob (Ceratonia siliqua) on sperm quality, testicular structure, testosterone level and oxidative stress in busulfan-induced infertile mice. Pharm Sci 2018; 24: 104-111.
[18] Meziani S, Oomah BD, Zaidi F, Simon-Levert A, Bertrand C, Zaidi-Yahiaoui R. Antibacterial activity of carob (Ceratonia siliqua L.) extracts against phytopathogenic bacteria Pectobacterium atrosepticum. Microb Pathog 2015; 78: 95-102.
[19] Rtibi K, Selmi S, Grami D, Saidani K, Sebai H, Amri M, et al. Ceratonia siliqua L. (immature carob bean) inhibits intestinal glucose absorption, improves glucose tolerance and protects against alloxan−induced diabetes in rat. J Sci Food and Agric 2017; 97: 2664-2670.
[20] Amessis-Ouchemoukh N, Ouchemoukh S, Meziant N, Idiri Y, Hernanz D, Stinco CM, et al. Bioactive metabolites involved in the antioxidant, anticancer and anticalpain activities of Ficus carica L., Ceratonia siliqua L. and Quercus ilex L. extracts. Industrial Crops and Products 2017; 95: 6-17.
[21] Faramarzi A, Aghaz F, Bakhtiari M, Khazaei M. In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men. Middle East Fertil Soc J 2020; 24: 1-5.
[22] Soleimanzadeh A, Kian M, Moradi S, Mahmoudi S. Carob (Ceratonia siliqua L.) fruit hydro-alcoholic extract alleviates reproductive toxicity of lead in male mice: Evidence on sperm parameters, sex hormones, oxidative stress biomarkers and expression of Nrf2 and iNOS. Avicenna J Phytomed 2020; 10: 35-49.
[23] World Health Organization. WHO laboratory manual for the examInation and processing of human semen. 6th Ed. Switzerland: World Health Organization Press; 2010.
[24] Practice Committee of the American Society for Reproductive Medicine. The clinical utility of sperm DNA integrity testing: A guideline. Fertil Steril 2013; 99: 673-677.
[25] Mohammadi F, Varanloo N, Heydari Nasrabadi M, Vatannejad A, Amjadi FS, Javedani Masroor M, et al. Supplementation of sperm freezing medium with myoinositol improve human sperm parameters and protects it against DNA fragmentation and apoptosis. Cell Tissue Bank 2019; 20: 77-86.
[26] Affonso FJ, Carvalho HF, Lançoni R, Lemes KM, Leite TG, Oliveira LZ, et al. Addition of antioxidants myoinositol, ferulic acid, and melatonin and their effects on sperm motility, membrane integrity, and reactive oxygen species production in cooled equine semen. J Equine Vet Sci 2017; 59: 57-63.
[27] Mangoli E, Talebi AR, Anvari M, Taheri F, Vatanparast M, Rahiminia T, et al. Vitamin C attenuates negative effects of vitrification on sperm parameters, chromatin quality, apoptosis and acrosome reaction in neat and prepared normozoospermic samples. Taiwan J Obstet Gynecol 2018; 57: 200-204.
[28] Souli A, Sebai H, Chehimi L, Rtibi K, Tounsi H, Boubaker S, et al. Hepatoprotective effect of carob against acute ethanol-induced oxidative stress in rat. Toxicol Ind Health 2015; 31: 802-810.
[29] Shabani Nashtaei M, Nekoonam S, Naji M, Bakhshalizadeh Sh, Amidi F. Cryoprotective effect of resveratrol on DNA damage and crucial human sperm messenger RNAs, possibly through 5′ AMP-activated protein kinase activation. Cell Tissue Bank 2018; 19: 87-95.
[30] Liu J, Wang W, Liu X, Wang X, Wang J, Wang Y, et al. Supplementation of cryopreservation medium with TATPeroxiredoxin 2 fusion protein improves human sperm quality and function. Fertil Steril 2018; 110: 1058-1066.
[2] Tao Y, Sanger E, Saewu A, Leveille M-C. Human sperm vitrification: The state of the art. Reprod Biol Endocrinol 2020; 18: 17.
[3] Aizpurua J, Medrano L, Enciso M, Sarasa J, Romero A, Fernández MA, et al. New permeable cryoprotectant-free vitrification method for native human sperm. Hum Reprod 2017; 32: 2007-2015.
[4] Le MT, Nguyen TTT, Nguyen TT, Nguyen VT, Nguyen TTA, Nguyen VQH, et al. Cryopreservation of human spermatozoa by vitrification versus conventional rapid freezing: Effects on motility, viability, morphology and cellular defects. Eur J Obstet Gynecol Reprod Biol 2019; 234: 14-20.
[5] Yeste M. Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology 2016; 85: 47-64.
[6] Karimfar MH, Niazvand F, Haghani K, Ghafourian S, Shirazi R, Bakhtiyari S. The protective effects of melatonin against cryopreservation-induced oxidative stress in human sperm. Int J Immunopathol Pharmacol 2015; 28: 69-76.
[7] Subramanian V, Ravichandran A, Thiagarajan N, Govindarajan M, Dhandayuthapani S, Suresh S. Seminal reactive oxygen species and total antioxidant capacity: Correlations with sperm parameters and impact on male infertility. Clin Exp Reprod Med 2018; 45: 88-93.
[8] Shaygannia E, Ghandehari-Alavijeh R, Tavalaee M, Nasr- Esfahani MH. The protective effects of alpha lipoic acid on human sperm function during freezing-thawing. Cryo Letters 2020; 41: 344-350.
[9] Najafi L, Halvaei I, Movahedin M. Canthaxanthin protects human sperm parameters during cryopreservation. Andrologia 2019; 51: e13389.
[10] Ahmadi S, Bashiri R, Ghadiri-Anari A, Nadjarzadeh A. Antioxidant supplements and semen parameters: An evidence-based review. Int J Reprod BioMed 2016; 14: 729-736.
[11] Berkovitz A, Allouche-Fitoussi D, Izhakov D, Breitbart H. Cryopreservation of human sperm in the presence of Zn2+ increases the motility rate. J Obstet Gynecol Investig 2018; 1: e6-e12.
[12] Truong TT, Gardner DK. Antioxidants increase blastocyst cryosurvival and viability post-vitrification. Hum Reprod 2020; 35: 12-23.
[13] Iovine C, Mottola F, Santonastaso M, Finelli R, Agarwal A, Rocco L. In vitro ameliorative effects of ellagic acid on vitality, motility and DNA quality in human spermatozoa. Mol Reprod Dev 2021; 88: 167-174.
[14] Santonastaso M, Mottola F, Iovine C, Colacurci N, Rocco L. Protective effects of curcumin on the outcome of cryopreservation in human sperm. Reprod Sci 2021; 28: 2895-2905.
[15] Faramarzi A, Aghaz F, Golestan Jahromi M, Bakhtiari M, Khazaei M. Does supplementation of sperm freezing/thawing media with Ceratonia siliqua improve detrimental effect of cryopreservation on sperm parameters and chromatin quality in normozoospermic specimens? Cell Tissue Bank 2019; 20: 403-409.
[16] Rtibi K, Jabri MA, Selmi S, Souli A, Sebai H, El-Benna J, et al. Gastroprotective effect of carob (Ceratonia siliqua L.) against ethanol-induced oxidative stress in rat. BMC Complement Altern Med 2015; 15: 292.
[17] Vafaei A, Mohammadi Sh, Fazel A, Soukhtanloo M, Mohammadipour A, Beheshti F. Effects of carob (Ceratonia siliqua) on sperm quality, testicular structure, testosterone level and oxidative stress in busulfan-induced infertile mice. Pharm Sci 2018; 24: 104-111.
[18] Meziani S, Oomah BD, Zaidi F, Simon-Levert A, Bertrand C, Zaidi-Yahiaoui R. Antibacterial activity of carob (Ceratonia siliqua L.) extracts against phytopathogenic bacteria Pectobacterium atrosepticum. Microb Pathog 2015; 78: 95-102.
[19] Rtibi K, Selmi S, Grami D, Saidani K, Sebai H, Amri M, et al. Ceratonia siliqua L. (immature carob bean) inhibits intestinal glucose absorption, improves glucose tolerance and protects against alloxan−induced diabetes in rat. J Sci Food and Agric 2017; 97: 2664-2670.
[20] Amessis-Ouchemoukh N, Ouchemoukh S, Meziant N, Idiri Y, Hernanz D, Stinco CM, et al. Bioactive metabolites involved in the antioxidant, anticancer and anticalpain activities of Ficus carica L., Ceratonia siliqua L. and Quercus ilex L. extracts. Industrial Crops and Products 2017; 95: 6-17.
[21] Faramarzi A, Aghaz F, Bakhtiari M, Khazaei M. In vitro application of Ceratonia siliqua improved sperm parameters and chromatin quality after vitrifacation in normozoospermic aged men. Middle East Fertil Soc J 2020; 24: 1-5.
[22] Soleimanzadeh A, Kian M, Moradi S, Mahmoudi S. Carob (Ceratonia siliqua L.) fruit hydro-alcoholic extract alleviates reproductive toxicity of lead in male mice: Evidence on sperm parameters, sex hormones, oxidative stress biomarkers and expression of Nrf2 and iNOS. Avicenna J Phytomed 2020; 10: 35-49.
[23] World Health Organization. WHO laboratory manual for the examInation and processing of human semen. 6th Ed. Switzerland: World Health Organization Press; 2010.
[24] Practice Committee of the American Society for Reproductive Medicine. The clinical utility of sperm DNA integrity testing: A guideline. Fertil Steril 2013; 99: 673-677.
[25] Mohammadi F, Varanloo N, Heydari Nasrabadi M, Vatannejad A, Amjadi FS, Javedani Masroor M, et al. Supplementation of sperm freezing medium with myoinositol improve human sperm parameters and protects it against DNA fragmentation and apoptosis. Cell Tissue Bank 2019; 20: 77-86.
[26] Affonso FJ, Carvalho HF, Lançoni R, Lemes KM, Leite TG, Oliveira LZ, et al. Addition of antioxidants myoinositol, ferulic acid, and melatonin and their effects on sperm motility, membrane integrity, and reactive oxygen species production in cooled equine semen. J Equine Vet Sci 2017; 59: 57-63.
[27] Mangoli E, Talebi AR, Anvari M, Taheri F, Vatanparast M, Rahiminia T, et al. Vitamin C attenuates negative effects of vitrification on sperm parameters, chromatin quality, apoptosis and acrosome reaction in neat and prepared normozoospermic samples. Taiwan J Obstet Gynecol 2018; 57: 200-204.
[28] Souli A, Sebai H, Chehimi L, Rtibi K, Tounsi H, Boubaker S, et al. Hepatoprotective effect of carob against acute ethanol-induced oxidative stress in rat. Toxicol Ind Health 2015; 31: 802-810.
[29] Shabani Nashtaei M, Nekoonam S, Naji M, Bakhshalizadeh Sh, Amidi F. Cryoprotective effect of resveratrol on DNA damage and crucial human sperm messenger RNAs, possibly through 5′ AMP-activated protein kinase activation. Cell Tissue Bank 2018; 19: 87-95.
[30] Liu J, Wang W, Liu X, Wang X, Wang J, Wang Y, et al. Supplementation of cryopreservation medium with TATPeroxiredoxin 2 fusion protein improves human sperm quality and function. Fertil Steril 2018; 110: 1058-1066.