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Victoria Rodríguez Gallego, M., Victoria Aparicio Prieto, M., Leza García, A., Hernández Hernández, J., Antonio Arizaleta Uralde, J., & Casis Sáenz, L. (2022). Metabolism of renin-angiotensin and enkephalin in human follicular fluid: An experimental study. International Journal of Reproductive BioMedicine (IJRM), 20(10), 851–860. https://doi.org/10.18502/ijrm.v20i10.12269

https://doi.org/10.18502/ijrm.v20i10.12269

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authors

  • Maria Victoria Rodríguez Gallego

    Maria Victoria Rodríguez Gallego

    Clínica Ginecológica Juana, Hernández, Logroño La Rioja Country, Spain.
  • Maria Victoria Aparicio Prieto

    Maria Victoria Aparicio Prieto

    Human Reproduction Unit, Cruces University Hospital, Barakaldo (Basque Country), Spain.
  • Andrea Leza García

    Andrea Leza García

    Clínica Ginecológica Juana, Hernández, Logroño La Rioja Country, Spain.
  • Juana Hernández Hernández

    Juana Hernández Hernández

    Clínica Ginecológica Juana, Hernández, Logroño La Rioja Country, Spain.
  • Jose Antonio Arizaleta Uralde

    Jose Antonio Arizaleta Uralde

    Clínica Ginecológica Juana, Hernández, Logroño La Rioja Country, Spain.
  • Luis Casis Sáenz

    Luis Casis Sáenz

    Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country (UPV/EHU), Bilbao, Spain.

Published Date

  • Nov 2, 2022

Metabolism of renin-angiotensin and enkephalin in human follicular fluid: An experimental study

International Journal of Reproductive BioMedicine (IJRM) / International Journal of Reproductive BioMedicine (IJRM): Volume 20, Issue No. 10 / Pages 851–860

Abstract

Background: The relationship between the biochemical characteristics of follicular fluid (FF), oocyte quality and embryonic development has not yet been elucidated. We compared samples of FF with a normal metabolic profile against samples with metabolic abnormalities to identify potential predictive biomarkers of reproductive success.


Objective: To analyze peptide activity in the FF of women undergoing in vitro fertilization using 3 samples of FF per individual.


Materials and Methods: FF samples were obtained by ovum pick-up. Pathological samples were defined as samples of FF obtained from women with a gynecological condition or with infertility. A total of 30 women participated in this study. 3 samples of FF were obtained per individual (90 samples), but 8 samples were excluded because they were hemolyzed. The samples (n = 82 FF) included controls (n = 36, donors without fertility problems), women with endometriosis (n = 15), unexplained infertility (n = 19), and aged > 39 (n = 12). We assessed local encephalinergics: aminopeptidase-N (puromycin sensitive aminopeptidase and neutral endopeptidase; and components of the angiotensin system of the reproductive tract: prolyl-endopeptidase, APN, aspartateaminopeptidase, and basic-aminopeptidase.


Results: No differences were observed in peptide metabolism based on the presence or absence of oocytes in the FF. Women with endometriosis and aged > 39 yr showed alterations in puromycin sensitive aminopeptidase (p = 0.01), aminopeptidase-B (p = 0.01), aspartate-aminopeptidase (p < 0.001) and neutral endopeptidase (p < 0.001).


Conclusion: This study reveals alterations in the metabolism of enkephalin and angiotensin in pathological FF, which points to these components as potential diagnostic biomarkers.


Key words: Follicular fluid, Peptides, Ovarian diseases, Women, Endometriosis, Infertility.

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