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Akmali, M., Yalmeh, R., Talaei-Khozani, T., Karimi, F., & Aliabadi, E. (2022). Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study. International Journal of Reproductive BioMedicine (IJRM), 20(4), 307-318. https://doi.org/10.18502/ijrm.v20i4.10903
https://doi.org/10.18502/ijrm.v20i4.10903
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authors
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Masoumeh Akmali
Masoumeh Akmali
Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
-
Roghayeh Yalmeh
Roghayeh Yalmeh
Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
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Tahereh Talaei-Khozani
Tahereh Talaei-Khozani
Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
-
Fatemeh Karimi
Fatemeh Karimi
Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
-
Elham Aliabadi
Elham Aliabadi
Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Published Date
- May 23, 2022
Effects of kisspeptin incubation on the mature mouse testicular sperms quality: An experimental study
Abstract
Background: Sperm quality has an important role in the success of assisted reproductive techniques, by adding some bioactive agents with a positive impact on sperms, it can be improved.
Objective: This study aimed to evaluate the effects of kisspeptin on the sperm motility criteria, Lactate dehydrogenase-C (LDHC) activity, acrosomal reaction, and capacitation in the mouse testicular sperm in vitro.
Materials and Methods: Sperm samples were extracted from testes of 96 male Balb/C mice weighing 25-30 gr, aged 6-8 wk. Then, they were separated into 4 parts; 2 controls and 2 kisspeptin-treated aliquots; each one incubated for either 15 or 30 min. The sperm motility and the LDHC activity were evaluated, and also the frequency of the non-capacitated, intact, and acrosomal-reacted sperms were evaluated by staining with Wheat germ agglutinin, Peanut agglutinin, and Concanavalin A, respectively. The stained sperms were analyzed by flow cytometry and fluorescent microscope.
Results: Our result showed that kisspeptin increased both the sperm motility (p = 0.04) and LDHC enzyme activity (p = 0.04) after 15 min of incubation. At the same time, it did not impact the frequency of the non-capacitated, intact and acrosomal-reacted sperms after incubation in the same period (p = 0.16).
Conclusion: A 15 min period of incubation with kisspeptin could be applicable for evaluating sperm motility and LDH activity.
Key words: Testis, Spermatozoa, Kisspeptin, Lactate dehydrogenase, Acrosome.
References
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[2] Gao Y, Zhang X, Xiong Sh, Han W, Liu J, Huang G. Motile sperm organelle morphology examination (MSOME) can predict outcomes of conventional in vitro fertilization: A prospective pilot diagnostic study. Hum Fertil 2015; 18: 258–264.
[3] Zhang Z, Zhu LL, Jiang HS, Chen H, Chen Y, Dai YT. Predictors of pregnancy outcome for infertile couples attending IVF and ICSI programmes. Andrologia 2016; 48: 874–881.
[4] Aliabadi E, Soleimani Mehranjani M, Borzoei Z, Talaei- Khozani T, Mirkhani H, Tabesh H. Effects of L-carnitine and L-acetyl-carnitine on testicular sperm motility and chromatin quality. Iran J Rprod Med 2012; 10: 77–82.
[5] Aliabadi E, Karimi F, Talaei-Khozani T. Effects of L-carnitine and pentoxifylline on carbohydrate distribution of mouse testicular sperm membrane. Iran J Med Sci 2013; 38: 107– 115.
[6] Bahmanpour S, Namavar MR, Talaei-Khozani T, Mazaheri Z. The effect of the follicular fluid on sperm chromatin quality in comparison with conventional media. Eur Rev Med Pharmacol Sci 2012; 16: 1840–1846.
[7] Aliabadi E, Jahanshahi S, Talaei-Khozani T, Banaei M. Comparison and evaluation of capacitation and acrosomal reaction in freeze-thawed human ejaculated spermatozoa treated with L-carnitine and pentoxifylline. Andrologia 2018; 50: e12845.
[8] Lee JY, Dada R, Sabanegh E, Carpi A, Agarwal A. Role of genetics in azoospermia. Urology 2011; 77: 598–601.
[9] Janosek-Albright KJC, Schlegel PN, Dabaja AA. Testis sperm extraction. Asian J Urol 2015; 2: 79–84.
[10] Cito G, Becatti M, Natali A, Fucci R, Picone R, Cocci A, et al. Redox status assessment in infertile patients with non-obstructive azoospermia undergoing testicular sperm extraction: A prospective study. Andrology 2020; 8: 364– 371.
[11] Meseguer M, Santiso R, Garrido N, Gil-Salom M, Remohí J, Fernandez JL. Sperm DNA fragmentation levels in testicular sperm samples from azoospermic males as assessed by the sperm chromatin dispersion (SCD) test. Fertil Steril 2009; 92: 1638–1645.
[12] Chianese R, Cobellis G, Chioccarelli T, Ciaramella V, Migliaccio M, Fasano S, et al. Kisspeptins, estrogens and male fertility. Curr Med Chem 2016; 23: 4070–4091.
[13] EL-sherry TM, Abdel-Ghani MA, Mahmoud GB, Ezzat AA. Kisspeptin injection improved the semen characteristics and sperm rheotaxis in Ossimi ram. Reprod Domest Anim 2020; 55: 240–247.
[14] Hsu MC, Wang JY, Lee YJ, Jong DS, Tsui KH, Chiu CH. Kisspeptin modulates fertilization capacity of mouse spermatozoa. Reproduction 2014; 147: 835–845.
[15] Björkgren I, Sipilä P. The impact of epididymal proteins on sperm function. Reproduction 2019; 158: R155–R167.
[16] Pinto FM, Cejudo-Román A, Ravina CG, Fernández- Sánchez M, Martín-Lozano D, Illanes M, et al. Characterization of the kisspeptin system in human spermatozoa. Int J Androl 2012; 35: 63–73.
[17] Chiang ChM, Chiu HY, Jong DSh, Wu LSh, Lee YJ, Chiu ChH. Role of the kisspeptin/ KISS1 receptor system in the testicular development of mice. J Chin Med Assoc 2021; 84: 203–211.
[18] Ni F, Yu H, Qu J, Meng L, Liu X, Yan W, et al. Hypothalamicpituitary- gonadal (HPG) axis and transcriptional regulatory elements regulate piwil2 gene expression during gametogenesis and gonadal development in Japanese flounder (paralichthys olivaceus). J Ocean Univ China 2020; 19: 1378–1388.
[19] Irfan S, Ehmcke J, Shahab M, Wistuba J, Schlatt S. Immunocytochemical localization of kisspeptin and kisspeptin receptor in the primate testis. J Med Primatol 2016; 45: 105–111.
[20] Yang F, Li L, Chen JP, Liu XQ, Zhong CL, Yang Y, et al. Couple’s infertility in relation to male smoking in a Chinese rural area. Asian J Androl 2017; 19: 311–315.
[21] Radovick S, Babwah AV. Regulation of pregnancy: Evidence for major roles by the uterine and placental kisspeptin/ KISS1R signaling systems. Semin Reprod Med 2019; 37: 182–190.
[22] Kudaibergenova A, Aydogdu N, Kandemir N, Aydin MA. Investigation of kisspeptin role in experimental kidney ischemia/reperfusion injury. Folia Med 2020; 62: 82–88.
[23] Akkaya H. Kisspeptin-10 administration regulates mTOR and AKT activities and oxidative stress in mouse cardiac tissue. J Evolution Biochem Physiol 2021; 57: 270–280.
[24] Hill TG. Kisspeptin: A novel regulator of glucose homeostasis during pregnancy. [Ph.D. thesis]. UK: King’s College London; 2019.
[25] Aliabadi E, Karimi F, Rasti M, Akmali M, Esmaeilpour T. Effects of L-carnitine and pentoxifylline on the activity of lactate dehydrogenase C4 isozyme and motility of testicular spermatozoa in mice. Int J Reprod BioMed 2013; 14: 56–61.
[26] Patel AS, Leong JY, Ramasamy R. Prediction of male infertility by the world health organization laboratory manual for assessment of semen analysis: A systematic review. Arab J Urol 2018; 16: 96–102.
[27] Fernandez-Fuertes B, Blanco-Fernandez A, Reid CJ, Meade KG, Fair S, Lonergan P. Removal of sialic acid from bull sperm decreases motility and mucus penetration ability but increases zona pellucida binding and polyspermic penetration in vitro. Reproduction 2018; 155: 481–492.
[28] Parodi J. Motility, viability, and calcium in the sperm cells. Syst Biol Reprod Med 2014; 60: 65–71.
[29] Correia J, Michelangeli F, Publicover S. Regulation and roles of Ca2+ stores in human sperm. Reproduction 2015; 150: R65–R76.
[30] Min L, Soltis K, Reis ACS, Xu S, Kuohung W, Jain M, et al. Dynamic kisspeptin receptor trafficking modulates kisspeptin-mediated calcium signaling. Mol Endocrinol 2014; 28: 16–27.
[31] Yoshimoto H, Takeo T, Irie T, Nakagata N. Fertility of coldstored mouse sperm is recovered by promoting acrosome reaction and hyperactivation after cholesterol efflux by methyl-beta-cyclodextrin. Biol Reprod 2017; 96: 446–455.
[32] Akkaya H, Kilic E, Eyuboglu Dinc S, Yilmaz B. Postacute effects of kisspeptin-10 on neuronal injury induced by Lmethionine in rats. J Biochem Mol Toxicol 2014; 28: 373– 377.
[33] Santoso B. The effect of sperm apoptosis, reactive oxygen species (ROS) and semen sample handling on sperm parameters and assisted reproductive technology (ART) outcomes. [Ph.D. thesis]. Australia: Edith Cowan University; 2020.
[34] Dutta S, Henkel R, Sengupta P, Agarwal A. Physiological role of ROS in sperm function. In: Parekattil SJ, Esteves SC, Agarwal. Male infertility. Switzerland: Springer; 2020. 337–345.
[35] Sharma A, Thaventhiran Th, Minhas S, Dhillo WS, Jayasena ChN. Kisspeptin and testicular function: Is it necessary? Int J Mol Sci 2020; 21: 2958.
[2] Gao Y, Zhang X, Xiong Sh, Han W, Liu J, Huang G. Motile sperm organelle morphology examination (MSOME) can predict outcomes of conventional in vitro fertilization: A prospective pilot diagnostic study. Hum Fertil 2015; 18: 258–264.
[3] Zhang Z, Zhu LL, Jiang HS, Chen H, Chen Y, Dai YT. Predictors of pregnancy outcome for infertile couples attending IVF and ICSI programmes. Andrologia 2016; 48: 874–881.
[4] Aliabadi E, Soleimani Mehranjani M, Borzoei Z, Talaei- Khozani T, Mirkhani H, Tabesh H. Effects of L-carnitine and L-acetyl-carnitine on testicular sperm motility and chromatin quality. Iran J Rprod Med 2012; 10: 77–82.
[5] Aliabadi E, Karimi F, Talaei-Khozani T. Effects of L-carnitine and pentoxifylline on carbohydrate distribution of mouse testicular sperm membrane. Iran J Med Sci 2013; 38: 107– 115.
[6] Bahmanpour S, Namavar MR, Talaei-Khozani T, Mazaheri Z. The effect of the follicular fluid on sperm chromatin quality in comparison with conventional media. Eur Rev Med Pharmacol Sci 2012; 16: 1840–1846.
[7] Aliabadi E, Jahanshahi S, Talaei-Khozani T, Banaei M. Comparison and evaluation of capacitation and acrosomal reaction in freeze-thawed human ejaculated spermatozoa treated with L-carnitine and pentoxifylline. Andrologia 2018; 50: e12845.
[8] Lee JY, Dada R, Sabanegh E, Carpi A, Agarwal A. Role of genetics in azoospermia. Urology 2011; 77: 598–601.
[9] Janosek-Albright KJC, Schlegel PN, Dabaja AA. Testis sperm extraction. Asian J Urol 2015; 2: 79–84.
[10] Cito G, Becatti M, Natali A, Fucci R, Picone R, Cocci A, et al. Redox status assessment in infertile patients with non-obstructive azoospermia undergoing testicular sperm extraction: A prospective study. Andrology 2020; 8: 364– 371.
[11] Meseguer M, Santiso R, Garrido N, Gil-Salom M, Remohí J, Fernandez JL. Sperm DNA fragmentation levels in testicular sperm samples from azoospermic males as assessed by the sperm chromatin dispersion (SCD) test. Fertil Steril 2009; 92: 1638–1645.
[12] Chianese R, Cobellis G, Chioccarelli T, Ciaramella V, Migliaccio M, Fasano S, et al. Kisspeptins, estrogens and male fertility. Curr Med Chem 2016; 23: 4070–4091.
[13] EL-sherry TM, Abdel-Ghani MA, Mahmoud GB, Ezzat AA. Kisspeptin injection improved the semen characteristics and sperm rheotaxis in Ossimi ram. Reprod Domest Anim 2020; 55: 240–247.
[14] Hsu MC, Wang JY, Lee YJ, Jong DS, Tsui KH, Chiu CH. Kisspeptin modulates fertilization capacity of mouse spermatozoa. Reproduction 2014; 147: 835–845.
[15] Björkgren I, Sipilä P. The impact of epididymal proteins on sperm function. Reproduction 2019; 158: R155–R167.
[16] Pinto FM, Cejudo-Román A, Ravina CG, Fernández- Sánchez M, Martín-Lozano D, Illanes M, et al. Characterization of the kisspeptin system in human spermatozoa. Int J Androl 2012; 35: 63–73.
[17] Chiang ChM, Chiu HY, Jong DSh, Wu LSh, Lee YJ, Chiu ChH. Role of the kisspeptin/ KISS1 receptor system in the testicular development of mice. J Chin Med Assoc 2021; 84: 203–211.
[18] Ni F, Yu H, Qu J, Meng L, Liu X, Yan W, et al. Hypothalamicpituitary- gonadal (HPG) axis and transcriptional regulatory elements regulate piwil2 gene expression during gametogenesis and gonadal development in Japanese flounder (paralichthys olivaceus). J Ocean Univ China 2020; 19: 1378–1388.
[19] Irfan S, Ehmcke J, Shahab M, Wistuba J, Schlatt S. Immunocytochemical localization of kisspeptin and kisspeptin receptor in the primate testis. J Med Primatol 2016; 45: 105–111.
[20] Yang F, Li L, Chen JP, Liu XQ, Zhong CL, Yang Y, et al. Couple’s infertility in relation to male smoking in a Chinese rural area. Asian J Androl 2017; 19: 311–315.
[21] Radovick S, Babwah AV. Regulation of pregnancy: Evidence for major roles by the uterine and placental kisspeptin/ KISS1R signaling systems. Semin Reprod Med 2019; 37: 182–190.
[22] Kudaibergenova A, Aydogdu N, Kandemir N, Aydin MA. Investigation of kisspeptin role in experimental kidney ischemia/reperfusion injury. Folia Med 2020; 62: 82–88.
[23] Akkaya H. Kisspeptin-10 administration regulates mTOR and AKT activities and oxidative stress in mouse cardiac tissue. J Evolution Biochem Physiol 2021; 57: 270–280.
[24] Hill TG. Kisspeptin: A novel regulator of glucose homeostasis during pregnancy. [Ph.D. thesis]. UK: King’s College London; 2019.
[25] Aliabadi E, Karimi F, Rasti M, Akmali M, Esmaeilpour T. Effects of L-carnitine and pentoxifylline on the activity of lactate dehydrogenase C4 isozyme and motility of testicular spermatozoa in mice. Int J Reprod BioMed 2013; 14: 56–61.
[26] Patel AS, Leong JY, Ramasamy R. Prediction of male infertility by the world health organization laboratory manual for assessment of semen analysis: A systematic review. Arab J Urol 2018; 16: 96–102.
[27] Fernandez-Fuertes B, Blanco-Fernandez A, Reid CJ, Meade KG, Fair S, Lonergan P. Removal of sialic acid from bull sperm decreases motility and mucus penetration ability but increases zona pellucida binding and polyspermic penetration in vitro. Reproduction 2018; 155: 481–492.
[28] Parodi J. Motility, viability, and calcium in the sperm cells. Syst Biol Reprod Med 2014; 60: 65–71.
[29] Correia J, Michelangeli F, Publicover S. Regulation and roles of Ca2+ stores in human sperm. Reproduction 2015; 150: R65–R76.
[30] Min L, Soltis K, Reis ACS, Xu S, Kuohung W, Jain M, et al. Dynamic kisspeptin receptor trafficking modulates kisspeptin-mediated calcium signaling. Mol Endocrinol 2014; 28: 16–27.
[31] Yoshimoto H, Takeo T, Irie T, Nakagata N. Fertility of coldstored mouse sperm is recovered by promoting acrosome reaction and hyperactivation after cholesterol efflux by methyl-beta-cyclodextrin. Biol Reprod 2017; 96: 446–455.
[32] Akkaya H, Kilic E, Eyuboglu Dinc S, Yilmaz B. Postacute effects of kisspeptin-10 on neuronal injury induced by Lmethionine in rats. J Biochem Mol Toxicol 2014; 28: 373– 377.
[33] Santoso B. The effect of sperm apoptosis, reactive oxygen species (ROS) and semen sample handling on sperm parameters and assisted reproductive technology (ART) outcomes. [Ph.D. thesis]. Australia: Edith Cowan University; 2020.
[34] Dutta S, Henkel R, Sengupta P, Agarwal A. Physiological role of ROS in sperm function. In: Parekattil SJ, Esteves SC, Agarwal. Male infertility. Switzerland: Springer; 2020. 337–345.
[35] Sharma A, Thaventhiran Th, Minhas S, Dhillo WS, Jayasena ChN. Kisspeptin and testicular function: Is it necessary? Int J Mol Sci 2020; 21: 2958.