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https://doi.org/10.18502/espoch.v1i3.9533

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authors

  • Anabell Del Rocío Urbina-Salazar

    Anabell Del Rocío Urbina-Salazar

    Universidad Técnica de Manabí, Instituto de Ciencias Básicas, Portoviejo, Ecuador
  • Alberto Renato Inca-Torres

    Alberto Renato Inca-Torres

    Universidad Técnica de Manabí, Instituto de Ciencias Básicas, Portoviejo, Ecuador
  • Bryan Anthony Urbina-Salazar

    Bryan Anthony Urbina-Salazar

    Universidad Técnica de Ambato, Facultad de Ciencias de la Salud, Carrera de Medicina, Ambato, Ecuador
  • Valeria Fernanda Inca-Torres

    Valeria Fernanda Inca-Torres

    Hospital Básico Baños, Baños, Ecuador
  • Juan Bautista

    Juan Bautista

    Universidad de Sevilla, Facultad de Farmacia, Departamento de Bioquímica y Biología Molecular, Sevilla, España

Published Date

  • Aug 29, 2021

Aqueous Extracts of Mushrooms Enriched with Ergothionein with Antioxidant Activity and Its Effect on Human Dermal Fibroblasts UV irradiated

ESPOCH Congresses: The Ecuadorian Journal of S.T.E.A.M. / Volume 1, Issue 3 / Pages 1072–1080

Abstract

Ergotioneine (ERG) has potential applications as food additive and as an antioxidant for the treatment and/or prevention of diseases related to oxidative stress. In this work, we study the nature of antioxidant activity and investigate the effect of aqueous extracts of Agaricus bisporus enriched with ergotioneine (EAHE-ERG), a natural product, obtained through enzymatic digestion and membrane technology, in cellular response induced by UV, in crops fibroblasts. EAHE-ERG suppressed by the increase of TNF-α by UV-B radiation. Furthermore, in fibroblasts exposed to UV-A, EAHE-ERG suppressed the expression of matrix metalloproteinase-1 protein (MMP-1) in almost 50% and reduced the RNAm expression from MMP-1. From these results, we conclude that EAHE-ERG can reduce anti-aging effects of the skin from UV radiation by removing O2 and 1O2, and the reduction protease signs and inflammatory activity.


Keywords: Ergotioneine, Agaricus bisporus, Antioxidant, Nutraceutical, Anti-inflammatory.


Resumen


La ergotioneina (ERG) tiene aplicaciones potenciales como un aditivo alimentario y como un antioxidante para el tratamiento y/o prevención de enfermedades relacionadas con el estrés oxidativo. En este trabajo estudiamos la naturaleza de la actividad antioxidante e investigamos el efecto de los extractos acuosos de Agaricus bisporus enriquecidos con ergotioneina (EAHEERG), un producto natural, obtenido mediante digestión enzimática y tecnología de membrana, en respuesta celular inducida por UV, en fibroblastos cultivados. EAHE-ERG suprimió la regulación por incremento de TNF-α mediante radiación UV-B. Además, en fibroblastos expuestos a UV-A, EAHE-ERG suprimió la expresión de proteína de metaloproteinasa-1 de matriz (MMP-1) en casi 50% y redujo la expresión de ARNm de MMP-1. A partir de estos resultados, concluimos que EAHE-ERG puede reducir los efectos antienvejecimiento de la piel después de la irradiación UV mediante la eliminación de O2 y 1O2, y la reducción de las señales de proteasa y actividad inflamatoria.


Palabras Clave: Ergotioneina, Agaricus bisporus, Antioxidante, Nutracéutico, Antiinflamatorio.

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