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Abdelgader, E. A., Eltayeb, N. H., Eltahir, T. A., Altayeb, O. A., Fadul, E. A., Ali, S. F., & Abdelrhman, E. M. (2020). Clinical Staging and Flowcytomteric CD38 and Zap 70 Prognostic Indicators in Sudanese Patients with Chronic Lymphocytic Leukemia . Sudan Journal of Medical Sciences (SJMS), 15(1), 43–55. https://doi.org/10.18502/sjms.v15i1.6704

https://doi.org/10.18502/sjms.v15i1.6704

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authors

  • Enaam Abdelrhman Abdelgader

    Enaam Abdelrhman Abdelgader

    Department of Pathology, Faculty of Medicine, Alneelain University, Sudan
  • Nada Hassan Eltayeb

    Nada Hassan Eltayeb

    Department of Physiology, Faculty of Medicine, Alneelain University, Sudan
  • Tasniem Ahmed Eltahir

    Tasniem Ahmed Eltahir

    Khartoum Oncology Hospital, Sudan
  • Osama Ali Altayeb

    Osama Ali Altayeb

    Flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis, Sudan
  • Eman Abbass Fadul

    Eman Abbass Fadul

    Flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis, Sudan
  • Soaad Fadlallah Ali

    Soaad Fadlallah Ali

    Department of Pathology, Faculty of Medicine, Gezira University, Sudan
  • Eldirdiri M. Abdelrhman

    Eldirdiri M. Abdelrhman

    Khartoum Oncology Hospital, Sudan

Published Date

  • Mar 31, 2020

Clinical Staging and Flowcytomteric CD38 and Zap 70 Prognostic Indicators in Sudanese Patients with Chronic Lymphocytic Leukemia

Sudan Journal of Medical Sciences (SJMS) / Sudan JMS: Volume 15 (2020), Issue No. 1 / Pages 43–55

Abstract

Background: The clinical course of chronic lymphocytic leukemia is highly variable. The determination of ZAP70 and CD38 is increasingly utilized as prognostic factor for chronic lymphocytic leukemia. The aim of conducting this study was to investigate the frequency of CD38 and ZAP70 expression among Sudanese Chronic lymphocytic leukemia (CLL) patients and to relate them to the Binet and Rai clinical staging systems.


Method: A total of 93 patients (mean age; 62.29 ± 11.68, sd) were enrolled in this cross-sectional study. CD38 and ZAP70 expression levels were measured with four color flowcytometry using the cut-off values of 20% for ZAP70 and 30% for CD38 expression. Staging was assessed by using clinical examination and CBC for all patients. Data were analyzed using the Statistical Package for Social science for Windows (SPSS), version 22.


Results: There were 93 CLL patients and the median age of the group was 63 years (36–95 years). About 71% of the patients presented with lymphadenopathy, 53.8% with splenomegaly, 73.1% with anemia, and 45.2% with thrombocytopenia. There was higher frequency of Binet stage C and Rai stage IV (62 [66.6%] patients and 34 [36.5%] patients, respectively). In addition, CD38 and ZAP70 showed higher frequency among Binet and Rai advance stages. ZAP70 and CD38 positivity were detected in 21 patients (22.6%) and 31 patients (33.3%), respectively. There was no statistically significant association between ZAP70 and CD38 and clinical staging systems (P-value > 0.05).


Conclusion: No significant association was observed between Flowcytometric (CD38 and Zap70) Prognostic Indicators and clinical staging systems.


Keywords: chronic lymphocytic Leukemia, Flowcytometry, ZAP70, CD38, clinical staging systems

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