Prevalence of endo-parasites of common tree frog (Rana Saharica) from Misurata, Libya


The current study was conducted to detect the prevalence of parasitic infection among (62) common tree frogs (Rana Saharica), of different ages, with weights ranged between 1 and 25g.

Results: The results revealed that 61 (98%) out of 62 examined common frogs were infected with endo-parasites. The prevalence of infection with protozoal was (86.4%) and (80%) for helminthes, while the mixed infection with both was 65%. Furthermore, the nematodes (75.5%) were the most prevalent helminthes, also helminthes mixed infection in a single host was reported. Rhabiditis spp., Ophiotaenia ranae, Glypthelmins quieta, Corynosoma spp. were Recovered from the digestive tract of common tree frogs, whereas, the Oplina ranarum and Copromonas subtillis the most prevalent protozoan species were detected from the common frogs. From the parasitic fauna seen in this study and from the statistical analysis, there were significant differences (P=0.05) between the weight of hosts and the density of infection.

[1] AlAttar, A.M. (2010): Hematological, Biochemical and Histopathological Studies on Marsh Frog, Rana ridibunda, Naturally Infected with Waltonella duboisi. International Journal of Zoological Research, 6: 199-213

[2] Bolek, M.G. and Coggins, J.R. (2003): Helminth community structure of sympatric eastern American toad, Bufo americanus americanus, northern leopard frog, Rana pipiens, and bluespotted salamander, Ambystoma laterale, from southeastern Wisconsin. Journal of Parasitology, 89, 673-680.

[3] Cable, R. M. (1985) An Illustarted laboratory manual of Parasitology 5th Ed. Surjeat pullications, Dehli, 255-269.

[4] Fatihu M., Ogboya, V.C., Njoku C.V. and Sanor D.I. (1991) Comparative studies of gastrointestinal helminths pf pourty in Zaria, Rev. Elev. Med. Vet. Pays. Trop., 44 (2): 175-177.

[5] Frost, D.R. (2010): Amphibian Species of the World: An Online Reference. Version 5.4 (8 April 2010). Am. Mus. Nat. Hist., New York. Available at

[6] Juncueira, L.C.U., Bignolas, G., Brentani, R.R., (1979): Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue section. Histochemical Journal, 11: 447–455.

[7] Kruse O.W. Pritchard M.N. (1982): Collection and preservation of animal parasites Technical bull.1 Univ. Nebraska lincolon and London, 141.

[8] Kusrini, M. D., Suzanna, E. and Satrija, F. (2003): Endoparasites of two species of edible frogs, Limnonectesmacrodon, Boie and Fejervaryacancrivora, Gravenhorst, from Bogor, Indonesia.Prosiding Seminar Hasil Penelitian Departemen Konservasi Sumberdaya Hutan, 53-64.

[9] McAllister, C.T., Bursey, C.R., Connior, M.B. and Trauth, S.E. (2013): Symbiotic Protozoa and Helminth Parasites of the Cajun Chorus Frog, Pseudacris fouquettei (Anura: Hylidae), from Southern Arkansas and Northeastern Texas, U.S.A. Comparative Parasitology, 80(1):96-104.

[10] McAllister, C.T., Connior, M.B. Bursey, C.R., Trauth, S.E., Robison, H.W. and Conn, D. B. (2014): Six New Host Records for Mesocestoides sp. Tetrathyridia (Cestoidea: Cyclophyllidea) from Amphibians and Reptiles of Arkansas, U.S.A Comparative parasitology, 81(2):278-283.

[11] Mcalpine, D.F. (1997): Helminth communities in bullfrogs (Rana catesbeiana), green frogs (Rana clamitans) and leopard frogs (Rana pipiens) from New Brunswick, Canada. Canadian Journal of Zoology, 75, 1883-1890.

[12] Muzzall, P.M. (1991): Helminth infracommunities of the frogs Rana catesbeiana and Rana clamitans from Turkey Marsh, Michigan. Journal of Parasitology, 77, 366 -371.

[13] Rahman, W.A. and Shakinah, Z. (2014): Endoparasitic Helminths of various Species of Frogs in Penang island, Peninsular Malaysia. International Journal of current Microbiology and Applied Science 3 ( 9): 933-939.

[14] Rahman, W.A., Tan, A. and Sufina, I. (2008): On the parasitic fauna of two species of anurans collected from Sungai Pinang, Penang Island, Malaysia. Tropical Biomedicine 25(2): 160–165.

[15] Tasawar Z., Aziz. and Akhtar M. (1999): Prevalence of cestode parasites of domestic fowl. Pakistan. Vet. 19 (3): 142-144.

[16] Vie, J.C., Hilton-Taylor, C., Stuart, S.N., (2009): Wildlife in a Changing World-an Analysis of the 2008 IUCN Red List of Threatened Species. International Union for Conservation of Nature, Gland, Switzerland.