https://doi.org/10.18502/sjms.v17i1.10689
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authors
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Muhammad Musthafa Poyil
Muhammad Musthafa Poyil
Department of Basic Medical Sciences, College of Medicine, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia
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Ponmurugan Karuppiah
Ponmurugan Karuppiah
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
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Suresh S. S. Raja
Suresh S. S. Raja
4Department of Microbiology, Government Arts and Science College, Perambalur, Tamil Nadu, India
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P. Sasikumar
P. Sasikumar
Department of Microbiology, The Madura College, Madurai, Tamil Nadu, India
Published Date
- Mar 31, 2022
Isolation, Extraction, and Characterization of Verotoxin-producing Escherichia coli O157:H7 from Diarrheal Stool Samples
Abstract
Background: Escherichia coli O157:H7 infection causes hemorrhagic colitis and is diagnosed based on symptoms such as cramps, stomach pain, and watery diarrhea. Shiga-like toxins (Verotoxin) produced by Escherichia coli O157:H7 damages endothelial cells of both kidney and brain, causing renal dysfunction and neurological problems.
Methods: The present study focuses on identifying the prevalence of Verotoxin-producing Escherichia coli O157:H7 among diarrheal inpatients at Erode Government Hospital, India, and its antibiogram. Further, the Verotoxins were characterized by using SDS-PAGE analysis. A total of 123 samples were collected both from diarrheal stools, and strains from 37 samples (43.02 %) were found to have the presence of E. coli. The organisms were identified based on their colony morphology on various media, cell morphology, and biochemical tests. The Shiga-like toxin production was identified by non-fermentation of sorbitol on SMAC agar plates. Confirmation of Shiga-like toxin was performed using agglutination assay.
Results: In total, 12 isolates showed agglutination and these isolates were confirmed to be E. coli O157:H7. The molecular weight of the Verotoxin was found to be between 20 and 29 kD. The antibiogram profile of the four isolated strains against 10 standard antibiotics was determined.
Conclusion: The results of this study show the occurrence of drug resistance on hemorrhagic colitis causing E. coli O157:H7.
Keywords:
E. coli, Verotoxin, diarrhea, hemorrhagic colitis
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