The Processing Model in Making Tempeh Benguk [Mucuna pruriens (L.) DC] Containing High L-Dopa
Abstract
“Koro benguk” [Mucuna pruriens (L.) DC] seed contains L-dopa which is able to repair spermatozoa quality. The aim of this experiment is to analyze L-Dopa using HPLC in engineering manufacture of tempeh benguk. There are four engineering model’s variants of making tempeh benguk. The engineering process begins with selection of koro benguk seed, tools required, immersion, boiling, humidity, temperature and pH. The result shows that the process in making tempeh benguk which contains the highest concentration of L-Dopa (11 346.85 mg · kg–1) is white-colored and intact koro benguk seed; one time immersion with replacing the water for 4 to 5 times/day (two days and two nights); one time boiling (< 1 h until koro benguk seed can be opened); mixed with 10 mL lime juice/1.5 kg, with concentration of 0.83 % until pH 5, added with mashed teak leaves, and ended with the process of making tempeh benguk at room temperature (30 ºC), relative humidity room (65 %). Tempeh benguk which is produced is cleaner, whiter, nonperishable, and more tender.
Keywords: HPLC; L-dopa; modified process of making; seeds benguk [Mucuna pruriens (L.) DC]; tempeh.
References
[1] Winarni., Analisis perbedaan pemeriksaan kimiawi untuk pemurnian l-dopa pada fraksi dan isolat biji koro benguk (Mucunapruriens L.) sebagai obat herbal untuk fertilitas [Difference analysis of chemical examination for purification of l-dopa on faction and isolate seeds koro surly (Mucunapruriens L.) medicinal herbs for fertility.]. Simposium Nasional VI-Litbangkes, 1 Winarni. Analisis perbedaan pemeriksaan kimiawi untuk
pemurnian l-dopa pada fraksi dan isolat biji koro benguk (Mucunapruriens L.) sebagai obat herbal untuk fertilitas [Difference analysis of chemical examination for purification of l-dopa on faction and isolate seeds koro surly (Mucunapruriens L.) medicinal herbs for fertility.]. Simposium Nasional VI-Litbangkes, Jakarta, 2010,
28 in Bahasa Indonesia.
[2] S. Suresh, E. Prithiviraj, and S. Prakash, “Dose- and time-dependent effects of ethanolic extract of Mucuna pruriens Linn. seed on sexual behaviour of normal male rats,” Journal of Ethnopharmacology, vol. 122, no. 3, pp. 497–501, 2009.
[3] K. K. Shukla, A. A. Mahdi, M. K. Ahmad, S. P. Jaiswar, S. N. Shankwar, and S. C. Tiwari, “Mucuna pruriens reduces stress and improves the quality of semen in infertile men,” Evidence-based Complementary and Alternative Medicine, vol. 7, no. 1, pp. 137– 144, 2010.
[4] M. K. Ahmad, A. A. Mahdi, K. K. Shukla, N. Islam, S. P. Jaiswar, and S. Ahmad, “Effect of Mucuna pruriens on semen profile and biochemical parameters in seminal plasma of infertile men,” Fertility and Sterility, vol. 90, no. 3, pp. 627–635, 2008.
[5] K. K. Shukla, A. A. Mahdi, M. K. Ahmad, S. N. Shankhwar, S. Rajender, and S. P. Jaiswar, “Mucuna pruriens improves male fertility by its action on the hypothalamus-pituitary-gonadal axis,” Fertility and Sterility, vol. 92, no. 6, pp. 1934– 1940, 2009.
[6] L. Misra and H. Wagner, “Extraction of bioactive principles from Mucuna pruriens seeds,” Indian Journal of Biochemistry and Biophysics, vol. 44, no. 1, pp. 56–60, 2007.
[7] NME. Cahyani, Daun Kemangi (Ocinum cannum) sebagai alternatif pembuatan hand sanitizier [Basil (Ocinum cannum) as an alternative preparation handsanitizer]. Jurnal Kesehatan Masyarakat, 136–142 [in Bahasa Indonesia], 9, 2014.
[8] GI. Haq, A. Permanasari, and H. Sholihin, “Efektivitas penggunaan sari buah jeruk nipis terhadap ketahanan nasi [Usage effectiveness against lime fruit extract endurance rice].,” Portal Jurnal Universitas Pendidikan Indonesia, vol. 1, no. 1, pp. 6–7, 2010, in Bahasa Indonesia.
[9] H. Nagayama, M. Hamamoto, M. Ueda, C. Nito, H. Yamaguchi, and Y. Katayama, “The effect of ascorbic acid on the pharmacokinetics of levodopa in elderly patients with Parkinson disease,” Clinical Neuropharmacology, vol. 27, no. 6, pp. 270–273, 2004.
[10] M. R. Wilkins, W. W. Widmer, and K. Grohmann, “Simultaneous saccharification and fermentation of citrus peel waste by Saccharomyces cerevisiae to produce ethanol,” Process Biochemistry, vol. 42, no. 12, pp. 1614–1619, 2007.
[11] B. Eni, M. Panut, and P. Suryo, “Pengaruh diameter partikel terhadap konsentrasi LDopa, kc, Dan De pada ekstraksi L-Dopa dari biji Kara Benguk (Mucuna Pruriens Dc.) [Effect of particle diameter of the concentration of l-dopa, kc, Dan De on extraction of L-Dopa from seeds velvet bean (Mucuna pruriens Dc.)],” Jurnal Kimia
dan Kemasan, vol. 35, pp. 123–129, 2013, in Bahasa Indonesia.