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Kusumawati, L., Wahyudi, R., Pinontoan, R., & Panggabean, M. G. L. (2015). SEQUENCE ANALYSIS OF 18s DNA OF <i>Melosira</i> sp., <i>Dunaliella</i> sp., <i>Isochrysis</i> sp. AND <i>Porphyridium</i&gt; sp. KnE Life Sciences, 2(1), 592-597. https://doi.org/10.18502/kls.v2i1.224

https://doi.org/10.18502/kls.v2i1.224

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authors

  • Lucia Kusumawati

    Lucia Kusumawati

    Department of Biotechnology and Neurosciences, Surya University, Jl. Scientia Boulevard Blok U/7, Serpong, Tangerang, 15810, Banten
  • Ruben Wahyudi

    Ruben Wahyudi

    Department of Biotechnology, University of Pelita Harapan. Jl. M.H. Thamrin Boulevard, Tangerang 15811, Banten
  • Reinhard Pinontoan

    Reinhard Pinontoan

    Department of Biotechnology, University of Pelita Harapan. Jl. M.H. Thamrin Boulevard, Tangerang 15811, Banten
  • Maria Gorreti Lily Panggabean

    Maria Gorreti Lily Panggabean

    Research Center for Oceanography, Indonesian Institute of Sciences (LIPI), Jl. Pasir Putih 1, Ancol Timur, Jakarta Utara 11048

Published Date

  • Sep 20, 2015

SEQUENCE ANALYSIS OF 18s DNA OF <i>Melosira</i> sp., <i>Dunaliella</i> sp., <i>Isochrysis</i> sp. AND <i>Porphyridium</i> sp.

KnE Life Sciences / International Conference on Biological Sciences (ICBS-2013) / Pages 592-597

Abstract

Phytoplankton has high level of biodiversity. In previous years phytoplankton was identified by their morphological characters. However, their morphology might change in different environments. These difficulties can be overcome by comparing their 18S rDNA sequences. This research is aimed to verify the identity of Melosira sp., Dunaliella sp., Isochrysis sp. and Porphyridium sp. Here, PCR method was used to amplify 18s DNA sequences. Three primer pairs were used, i.e. 18S-F and 18S-R; 501F and 1700R; 18S-2F and 18S-2R. PCR products were sequenced. MEGA5 was used to make phylogenetic tree. Genus verification for Isochrysis sp., Dunaliella sp. and Melosira sp. were conducted successfully using Blast and phylogenetic tree. 18s DNA sequence of Porphyridium sp. shows an interesting result and needs further verification.


Keywords: Phytoplankton, Melosira sp., Dunaliella sp., Isochrysis sp., Porphyridium sp.

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