Skin Stem Cell Resource Potential for Peripheral Nerve Repair Due to trauma of post regional anasthesia
Abstract
This study is undertaken to investigate the utilization of rabbit skin to be used as a source of stem cell, especially stem cells that is derived from the skin, to provide the healing and connection of damaged peripheral nerves. Based on the existing studies, there are many failures in the healing and connection of nerve due to trauma after regional anesthesia, and there is an opportunity that stem cells from the skin is potential to help the process of healing and even improvement in the failure of nerve grafting. 24 rabbits is used as experimental animals in this research. The 24 rabbit devided into 2 groups, the first group is a control group without suplementary therapy of rabbit skin stem cells, and the second group is the treated group that given rabbit skin stem cell as a supplementary therapy to treat the peripheral nerve damage post ansthesia. The result of this study shows that there is a significant differences between control and treated group. Statistical analysis was performed by using reliability test - intraclass correlation coefficient to assess interobserver variation and investigate the correlation.
Keywords : Stem Cell, Skin, Peripheral Nerve, Nerve Repair.
References
P. Audebert, P. Hapiot, J. Electroanal. Chem. 361 (1993) 177.
J. Newman, Electrochemical Systems, 2nd ed., Prentice-Hall, Englewood Cliffs, NJ, 1991.
A.R. Hillman, in: R.G. Linford (Ed.), Electrochemical Science and Technology of Polymers, vol. 1, Elsevier, Amsterdam, 1987, Ch. 5.
B. Miller, Proc. 6th Australian Electrochem. Conf., Geelong, Vic., 19-24 Feb., 1984; J. Electroanal. Chem., 168 (1984) 91.
Jones, personal communication, 1992.
Walsh S, Midha R. Practical Considerations Concerning the Use of Stem Cells for Peripheral Nerve Repair. Neurosurg Focus 2. 2009.
Linsley MD, Ekinci FJ, Ortiz D, 2005. Monitoring Thiobarbituric Acid-Reactive Substances (TBARs) as an assay for oxidative damage in neuronal cultures and central nervous system. Journal of Neuroscience Methods 141 : 219-222.
Rantam FA, Ferdiansyah, Purwati, 2014. Stem cell mesenchymal, hematopoetik dan model aplikasi. 2nd Ed. Surabaya: Airlangga University Press, P. 38.
Rokhind, S. 2009. Phototherapy in peripheral nerve regeneration: From basic science to clinical study. Journal of Neurosurgery 28 : (3).
[9] Toma JG, McKenzie IA, Bagli D, Miller FD 2005. Isolation and Characterization of Multipotent Skin-Derived Precursors from Human Skin. Stem Cells 23: 727-737
[10]Mc Kenzie IA, Biernaskie J, Toma JG, Midha R, Miller FD ,2006. Skin Derived Precursors Generate Myelinating Schwann Cells for the Injured and Dysmyelinated Nervous System. The Journal of Neuroscience 24 : 6651-6660.
[11]Hunt DPJ, Morris PN, Sterling J, 2008. A Highly Enriched Niche of Precursor Cellswith Neuronal and Glial Potential Within the Hair Follicle Dermal Papilla of AdultSkin
[12]Gago N. Pérez-López V. Sanz-Jaka JP. Cormenzana P. Eizaguirre I. Bernad A. Izeta A. Age-dependent depletion of human skin-derived progenitor cells. Stem Cells. 2009;27:1164–1172. [PubMed]
[13] Morris RJ. Liu Y. Marles L. Yang Z. Trempus C. Li S. Lin JS. Sawicki JA. Cotsarelis G. Capturing and profiling adult hair follicle stem cells. Nat Biotechnol. 2004;22:411–417. [PubMed]
[14] Chen FG. Zhang WJ. Bi D. Liu W. Wei X. Chen FF. Zhu L. Cui L. Cao Y. Clonal analysis of nestin(−) vimentin(+) multipotent fibroblasts isolated from human dermis. J Cell Sci. 2007;120:2875–2883. [PubMed]
[15] Kim HS, Oh SK, Park YB, 2005. Methods for deprivation of human embrionic stem cells.Stem Cells ; 23: 1228-33.
[16]Kamadjaja DB. Purwati. Rantam FA. Ferdiansyah. Coen P.The Osteogenic Capacity of Human Amniotic Membrane Mesenchymal Stem Cell (hAMSC) and Potential for Application in Maxillofacial Bone Reconstruction in Vitro Study. J. Biomedical Science and Engineering, 2014, 7, 497-503
[17] Linsley MD, Ekinci FJ, Ortiz D, 2005. Monitoring Thiobarbituric Acid-Reactive Substances (TBARs) as an assay for oxidative damage in neuronal cultures and central nervous system. Journal of Neuroscience Methods 141 : 219-222.
[18] Mc Kenzie IA, Biernaskie J, Toma JG, Midha R, Miller FD ,2006. Skin Derived Precursors Generate Myelinating Schwann Cells for the Injured and Dysmyelinated Nervous System. The Journal of Neuroscience 24 : 6651-6660
[19] Fernandes KJ. McKenzie IA. Mill P. Smith KM. Akhavan M. Barnabé-Heider F. Biernaskie J. Junek A. Kobayashi NR, et al. A dermal niche for multipotent adult skin-derived precursor cells. Nat Cell Biol. 2004;6:1082–1093.
[20] Burnett Mgand Zager El, 2004 .Pathophysiology of peripheral nerve injury: a brief review, Neurosurg Focus 16 (5):Article 1
[21]Radtke C Schmitz B . Spies M , Kocsis JD , Vogt PM Peripheral glial cell differentiation from neurospheres derived from adipose mesenchymal stem cells. International Journal of Developmental Neuroscience. 27(8): 817-823
[22] Rochkind S. Nevo Z. 2014. Recovery of Peripheral Nerve with Massive Loss Defect by Tissue Engineered Guiding Regenerative Gel. Biomed Research International.