ISOLATION OF <i>FLAVONOID 3’5’HYDROXILASE</i> GENE FROM SNAKEWEED (<i>Stachytarpheta indica</i> auct. non. (L.) Vahl)
Abstract
Stachytarpheta is one of Indonesian medicinal plants which have a wide species diversity. The bioactive component in snakeweed herb including naringenin, flavonol, and flavon are mainly derivate from phenyl propanoid (flavonoid). The gene encodes F3’5’Hydroxilation (F3’5H) involved in the biosynthesis of flavonoid product, by means of catalysis hydroxylation on the C atom #3 and #5 of benzene ring. Recently, has not yet meet publication about F3’5’H from snakeweed herb. The aim of this study was to isolate F3’5H gene from Stachytarpheta indica, using primers which were designed from conserve region of Petunia hybrida F3’5’H gene; HF1 and HF2 alleles. Forward primer is 5'-TGATGCTGCTAAAGCATTCT-3' and reverse primer is 5’GTGCACGCAGGTGACATATG-3’. The amplified fragments were un-specific non-consensus sequences, suggested that two homolog gene locus were isolated. Sequence analysis showed that both share two different domains; conserve-upstream and homolog-downstream domains. It is suggested that Stachytarpheta indica may possesses different alleles for F3’5’H, i.e. HF1 and HF2, as of Petunia hybrida.
Keywords: F3’5’H gene, Stachytarpheta indica, snakeweed
References
Bogs, J., A. Ebadi, D. McDavid, and Robinson. 2006. Identification of The Flavonoid Hydroxylases From Grapevine and Their Regulation During Fruit Development. Plant Physiology 140:279-291.
Dovichi, S., and F. Lajolo. 2011. Flavonoid and Their Relationship to Diseases of The Central Nervous System. Journal Brazilian Sociaty Food Nutrition Sao Paulo 36: 123-135.
Ferreyra, M., S. Rius, and P. Casati. 2000. Flavonoid: Biosynthesis, Biological Function, and Biological Application. Plant Science 3:1-15.
McGill. 2012. Trubleshooting Sequencing Product. McGill University and Genome Quebec innovation Centre Sequencing Service.
Mol, J., E. Grotewold, and R. Koes. 1999. How Genes Paint Flowers and Seeds. Trends Plant Science 3: 212-217.
Olsen, K., A. Hehn, H. Judge, R. Slimestad, R. Larbat, F. Bourgaud, and C. Lillo. 2010. Identification and Characterization of CYP75A31, A New Flavonoid 3’5’Hydroxilase Isolated From Solanum Lycopersicum. Plant Biology 10: 21-26.
Thompson, B.D. 2006. Finding Homologous Genes With Primers Designed Using Evolutionary Models. Dissertation. North Caroline State University.
Winkel-Shirley, B. 2010. Flavonoid Biosythesis A Colourfull Model for Genetics, Biochemistry, Cell Biology, and Biotechnology. Plant Phsiology 126:485-493.
Thomas, A., Brugliera, Lester, Tanaka, L. Menthing, and C. Stevenson. 1993. Cloning and Expression of Cytochrome P450 Genes Controlling Flower Colour. Nature