Bioremediation of Mercury (II) Contaminated Seawater Using the Diatom Skeletonema costatum

Abstract

The mercury contaminated seawater can pollute fish pond. Bioremediation is an effective process for the removal and recovery of mercury (II) from seawater using organism as an agent of biological degradation. The aim of this study was to know the optimum contact time and concentrations of the Skeletonema costatum cell inoculation on the bioremediation in mercury (II) contaminated seawater. This study has used the concentrations of the cell inoculation (5000; 10000; and 15000 cells/mL), the mercury (II) (0; 0.5; 1; and 2 mg/L), the contact time (24, 48, 72, 96, and 120 hours), and its replicated five times. The maximum bioremediation capacity of mercury (II) was 2 mg/L at 15 000 cells/mL and contact time 96 hours, with bioremediation efficiency 86.83%. Diatom Skeletonema costatum was efficient at removing 2 mg/L mercury (II) 79.5% for 5 000 cells/L at 72 hours, 83.3 % for 10 000 cells/L at 72 hours and 85% for 15 000 cells/L at 72 hours. The optimum contact time and concentrations of the Skeletonema costatum cell inoculation on the bioremediation in mercury (II) contaminated seawater for 2 mg/L, i.e. 5 000 cells/L for 72 hours (79.5%)

 

Keywords: bioremediation; mercury (II); seawater; Skeletonema costatum.

References
[1] M. Waldichuck, "Pollution and physiology of marine organisms" in Some Biological Concern in Metals Pollution, VERNBERG & VERNBERG eds., Acad. Press, London, 1974, pp. 1-45.

[2] Palar H., Pencemaran dan Toksikologi Logam Berat, Rineka Cipta, Jakarta, 2008.

[3] Y. Nassiri, J.L. Mansot, J. We´ ry, T. Ginsburger-Vogel, and J.C. Amiard, Ultrastructural, and electron energy loss spectroscopy studies of sequestration mechanisms of Cd and

Cu in the marine diatom Skeletonema costatum. Arch. Environ.

Contam. Toxicol. 33 (1997) 147–155.

[4] H.V. Perales-Vela, J.M. Pena-Castro, and R.O. Canizares-Villanueva, Heavy metal detoxification in eukaryotic microalgae, J. Chemosphere 64, (2006) 1-10.

[5] R. Leonard, Studi Perbandingan Kemampuan Skeletonema sp. dan Chaetoceros sp. Sebagai Agen Bioremediasi Terhadap Logam Berat Merkuri (Hg). Skripsi. Program Studi Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Surabaya, 2014.

[6] B. Priadie, 2012, Teknik Bioremediasi Sebagai Alternatif Dalam Upaya Pengendalian Pencemaran Air. Jurnal Ilmu Lingkungan 10 (2012) 38-48.

[7] Suhendrayatna,“HeavyMetalBioremovalby

Microorganisms,” in A Literature Study. Http://www.istecs.org/Publication/Japan/010211_suhendraya tna.pdf, 2011, [cited on 12/04/2017].

[8] Knauer, K. R. Behra, and L. Sigg, Adsorption and Uptake of Copper by the Green Algae Scendesmus subspicatus (Chlorophyta). Journal Phycol. 1997.

[9] G.R. Hasle, E.E. Syvertsen, K.A. Steidinger, and K. Tangen, in: C.R. Tomas (Ed.). Identifying Marine Diatoms and Dinoflagellates, Academic Press, Inc. California, 1996, Ch. 2.

[10] T. Soedarti, L.R. Maryono, and S. Hariyanto, 4th International Conference on Sustainable Built Environment (ICSBE), Yogyakarta, Oct 12th -14th, 2016; Proc. ICSBE http://icsbe.uii.ac.id/proceeding-icsbe-2016/, 27.

[11] P.J. Harrison and J.A. Berges, in: R.A. Andersen (Ed.), Algal Culturing Techniques, Elsevier, China, 2005, Ch. 3.

[12] Y. Hala, Emma S., dan Paulina T., Biosorpsi Campuran Logam

Pb2+ dan Zn2+ Oleh Chaetoceros calcitrans, Universitas Hasanuddin, Makassar, 2012.

[13] Gupta, R. P., Ahuya, S. Khan, R. K. Sakena and H. Mohapatara, 2000, Microbial Biosorbent: Meeting Challenges of Heavy Metal Pollution in Aqueous Solution. Current Science, 78 (2000) 8.



Biotechnology Strengthen on Biomedical Science and Veterinary Medicine
5

[14] Fauziah, Efektivitas Penyerapan Logam Kromium (Cr IV) dan Kadmium (Cd) oleh Scendesmus dimorphus. S1. Skripsi.

Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah, Jakarta, 2011.