Molecular Identification of Pathogen Yeast from Star Gooseberry (Phyllanthus acidus) and Cucumber (Cucumis sativus L.) Extracts
Abstract
In this study, two yeast strains, isolated from spontaneously fermented extracts of cucumber and star gooseberry fruits, were characterized by molecular methods. The identification of yeast isolates at the species level performed with sequence-based analysis of the region spanning the D1 and D2 regions (D1/D2) of the large ribosomal subunit (LSU) allowed for more accurate identification of yeast species. The yeasts identification proceeds based on the sequencing of the polymerase chain reaction (PCR)-amplified 600 base pair D1/D2 region of the yeast 26S ribosomal DNA, which was compared by a BLAST search to the D1/D2 regions of all validly described yeast species on file in the GenBank database, facilitating accurate and rapid identification. This research identified the yeasts from spontaneous fermentation of extract star gooseberry and cucumber fruits as Candida tropicalis (100%, 637 bp) and Kodama ohmeri (99%, 539bp), respectively. Both of the yeasts were pathogen yeast in fruits and humans.
Keywords: molecular identification, star gooseberry, cucumber
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