Isolation of Lauric Acid and Caffeine From Durio kutejensis Root Bark


Indonesia especially in Kalimantan has many herbs that are used by the people as traditional medicine such as pampaken (Durio kutejensis) and secondary metabolite in the root bark has potential to be explore. However, the study of Durio kutejensis root bark has not been reported. The objectives of this study were to isolate and identify secondary metabolite compounds of Durio kutejensis root bark. Secondary metabolite compounds of Durio kutejensis root bark were obtained with extraction, fractionation and purification process while identification with LCMS, 1D NMR, and 2D NMR analysis. The results were showed that the Durio kutejensis root bark contained lauric acid and caffeine.

Keywords: Lauric acid; caffeine; herbs; traditional medicine

[1] Rajkumar V, Guha G, Kumar RA. Isolation and bioactivity evaluation of two metabolites from the methanolic extract of Oroxylum indicum stem bark. Asian Pac. J. Trop. Biomed. 2012;S7–S11.

[2] Brusottia G, Cesaria I, Dentamaroa A, Caccialanzaa G, Massolinia G. Isolation and characterization of bioactive compounds from plant resources: The role of analysis in the ethnopharmacological approach. J. Pharm. Biomed. Anal. 2013;1–11.

[3] Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. Chin. Med. 2018;13(20):1–26.

[4] Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. Int. Pharm. Sci. 2011;1(1):98–106.

[5] Atmoko T. Potency and conservation of Wild Durian of Kalimantan (Durio kutejensis). Conf. Pap. 2014;437–448.

[6] Gorinstein S et al. Antioxidant properties and bioactive constituents of some rare exotic Thai fruits and comparison with conventional fruits In vitro and in vivo studies. Food Res. Int. 2011;44:2222–2232.

[7] Sardans J, Llusia J, Owen SM, Niinemets U. Screening study of leaf terpene concentration of 75 borneo rainforest plant species: relationships with leaf elemental concentrations and morphology. Rec. Nat. Prod. 2015;9(1):19–40.

[8] Khoo HE, Azlan A, Kong KW, Ismail A. Phytochemicals and medicinal properties of indigenous tropical fruits with potential for commercial development, evidencebased complement. Altern. Med. 2016;2016:1–20.

[9] Rudiansyah, Garson MJ. Secondary metabolites from the wood bark of durio zibethinus and durio kutejensis. J. Nat. Prod. 2006;69:1218–1221.

[10] Ikram EHK et al. Antioxidant capacity and total phenolic content of Malaysian underutilized fruits. Journal of Food Composition and Analysis. 2009;22:388–393.

[11] Arung ET et al. Determination of antioxidant and anti-melanogenesis activities of indonesian lai, durio kutejensis [bombacaceae (hassk) becc] fruit extract. Trop. J. Pharm. Res. 2015;14( January):41–46.

[12] Yusro F, Ohtani K, Kubota S. Inhibition of α-glucosidase by methanol extracts from wood bark of anacardiaceae , fabaceae , malvaceae and phyllanthaceae plants family in West Kalimantan, Indonesia. Kuroshio Sci. 2016;9(2):108–122.

[13] Peedikayil FC, Remy V, John S, Chandru TP, Sreenivasan P, Bijapur GA. Comparison of antibacterial efficacy of coconut oil and chlorhexidine on Streptococcus mutans: An in vivo study. J. Int. Soc. Prev. Community Dent. 2016;6(5):447–452.

[14] Pradhan D, Biswasroy P, Pradhan R. Qualitative and quantitative analysis of caffeine in some commercial brands of tea consumed in India. J. Ayurvedic Herb. Med. 2017;3(4):200–204.

[15] Abe M, Ito Y, Suzuki A, Onoue S, Noguchi H, Yamada S. Isolation and pharmacological characterization of fatty acids from saw palmetto extract. Anal. Sci. 2009;25(4):553–557.

[16] Nitbani FO, Jumina, Siswanta D, Solikhah EN. Isolation and antibacterial activity test of lauric acid from crude coconut oil (cocos nucifera L.). Procedia Chem. 2016;18(February):132–140.

[17] Verma R, Kumar L. Characterization of caffeine isolated from camellia sinensis leaves. J. Chem. Pharm. Res. 2010;2(4):194–198.

[18] Yang X, Zhou SL, Ma AC, Xu HT, Guan HS, Liu HB. Chemical profiles and identification of key compound caffeine in marine-derived traditional Chinese medicine Ostreae concha. Mar. Drugs. 2012;10(5):1180–1191.