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Lubis, H. P., & Halim, B. (2016). In Vitro Fertilization Outcome After Intracytoplasmic Sperm Injection with Fresh and with Frozen-Thawed Epididymal Spermatozoa. KnE Medicine, 1(1), 25-31. https://doi.org/10.18502/kme.v1i1.533

https://doi.org/10.18502/kme.v1i1.533

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authors

  • Hilma Putri Lubis
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  • Binarwan Halim
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Published Date

  • Oct 4, 2016

In Vitro Fertilization Outcome After Intracytoplasmic Sperm Injection with Fresh and with Frozen-Thawed Epididymal Spermatozoa

KnE Medicine / The 6th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2016) / Pages 25-31

Abstract

Introduction

Testicular epididymal sperm aspiration (TESA) is one of the method  to retrieve sperm from the testes in men with azoospermia. The aim of the study is to compare the In vitro fertilization (IVF) outcome of intracytoplasmic sperm injection (ICSI)-ET cycles with fresh testicular epididymal spermatozoa obtained on the same day with  oocyte retrieval and with frozen-thawed testicular epididymal spermatozoa.

Material & Methods

A retrospective comparative analysis of  patients who underwent fresh TESA and frozen-thawed TESA in ICSI-ET cycles from January 2012 to December 2014 in Halim Fertility Center was done. Fresh testicular epididymal sperm aspiration (fresh TESA) was performed on the same day with oocyte retrieval in 28 cycles and the frozen-thawed testicular epididymal sperm aspiration (frozen-thawed TESA) was used in 30 cycles.  

Results

The two groups were comparable in terms of the ages of male and female patients, etiology of infertility and duration of infertility. Fertilization rates in fresh TESA group were 53,5% and in frozen-thawed TESA group, fertilization rates were 50%. There was no statistically significant difference between the groups. Clinical pregnancy rates in fresh TESA group were 35,7%  and in frozen-thawed TESA group, clinical pregnancy rates were 26,7% and statistically there was no significant difference between the groups.

Conclusion

There is no significant difference in the in vitro fertilization outcome of intracytoplasmic sperm injection (ICSI)-ET cycles between fresh TESA and frozen-thawed TESA .

References
[1] M. Karacan, F. Alwaeely, S. Erkan, Z. Çebi, M. Berberolugil, M. Batukan, M. Ulu, A. Arvas, and T. Çamlibel, Outcome of intracytoplasmic sperm injection cycles with fresh testicular spermatozoa obtained on the day of or the day before oocyte collection and with cryopreserved testicular sperm in patients with azoospermia,
Fertility and Sterility, 100, no. 4, 975–980, (2013).


[2] S. Friedler, D. Strassburger, A. Raziel, D. Komarovsky, Y. Soffer, and R. Ron-El, Intracytoplasmic injection of fresh and cryopreserved testicular spermatozoa in patients with nonobstructive azoospermia - A comparative study, Fertility and Sterility, 68, no. 5, 892–897, (1997).


[3] R. Schoysman, P. Vanderzwalmen, M. Nijs, L. Segal, G. Segal-Bertin, L. Geerts, E. van Roosendaal, and D. Schoysman, Pregnancy after fertilisation with human testicular spermatozoa, The Lancet, 342, no. 8881, p. 1237, (1993).


[4] S. J. Silber, A. C. Van Steirteghem, J. Liu, Z. Nagy, H. Tournaye, and P. Devroey, High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsy, Human Reproduction, 10, no. 1, 148–152, (1995).


[5] I. De Croo, J. Van Der Elst, K. Everaert, P. De Sutter, and M. Dhont, Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia, Human Reproduction, 15, no. 6, 1383–1388, (2000).


[6] B. Balaban, B. Urman, A. Isiklar, C. Alatas, R. Mercan, S. Aksoy, and A. Nuhoglu, Blastocyst transfer following intracytoplasmic injection of ejaculated, epididymal or testicular spermatozoa, Human Reproduction, 16, no. 1, 125–129, (2001).


[7] M. A. Aboulghar, A. Kamal, R. T. Mansour, N. A. Tawab, G. I. Serour, Y. M. Amin, and I. Fahmy, Fertilization and pregnancy rates after intracytoplasmic sperm injection using ejaculate semen and surgically retrieved sperm, Fertility and Sterility, 68, no. 1, 108–111, (1997).


[8] J. D. M. Nicopoullos, C. Gilling-Smith, P. A. Almeida, J. Norman-Taylor, I. Grace, and J. W. A. Ramsay, Use of surgical sperm retrieval in azoospermic men: A meta-analysis, Fertility and Sterility, 82, no. 3, 691–701, (2004)


[9] A. Hourvitz, A. Shulman, I. Madjar, J. Levron, D. Levran, S. Mashiach, and J. Dor, In vitro fertilization treatment for severe male factor: A comparative study of intracytoplasmic sperm injection with testicular sperm extraction and with spermatozoa from ejaculate, Journal of Assisted Reproduction and Genetics, 15, no.
6, 386–389, (1998).


[10] H. Tournaye, T. Merdad, S. Silber, H. Joris, G. Verheyen, P. Devroey, and A. Van Steirteghem, No differences in outcome after intracytoplasmic sperm injection with fresh or with frozen-thawed epididymal spermatozoa, Human Reproduction, 14, no. 1, 90–95, (1999).


[11] I. Craft and M. Tsirigotis, Simplified recovery, preparation and cryopreservation of testicular spermatozoa, Human Reproduction, 10, no. 7, 1623–1626, (1995).


[12] M. Wald, L. S. Ross, G. S. Prins, J. Cieslak-Janzen, G. Wolf, and C. S. Niederberger, Analysis of outcomes of cryopreserved surgically retrieved sperm for IVF/ICSI, Journal of Andrology, 27, no. 1, 60–65, (2006).


[13] S. Cayan, D. Lee, J. Conaghan, C. A. Givens, I. P. Ryan, E. D. Schriock, and P. J. Turek, A comparison of ICSI outcomes with fresh and cryopreserved epididymal spermatozoa from the same couples, Human Reproduction, 16, no. 3, 495–499, (2001).


[14] E. N. Tavmergen Göker, F. Sendag, R. Levi, H. Sendag, and E. Tavmergen, Comparison of the ICSI outcome of ejaculated sperm with normal, abnormal parameters and testicular sperm, European Journal of Obstetrics Gynecology and Reproductive Biology, 104, no. 2, 129–136, (2002).