Characteristics, Antioxidant Activity, and Sun Protection Factor of Black Pomegranate Peel Ethanolic Extract Hydrogel
Abstract
Solar UV radiation can inactivate the SARS-CoV-2 virus and indirectly accelerate the recovery rate of Covid-19 patients by converting pro-vitamin D3 in the skin into pre-vitamin D. On the other hand, prolonged exposure to UV radiation might be harmful to skin health, making it necessary to use sunscreen. Synthetic chemical sunscreens can have adverse side effects, and alternatives utilizing natural ingredients are needed. The peel of the black pomegranate has strong antioxidant properties and it also has the potential as a sunscreen by making it into a topical hydrogel preparation. The purpose of this study was to examine the characteristics, antioxidant activity, and sun protection factor (SPF) capacity of the ethanol extract of black pomegranate peel hydrogel preparations. Hydrogel was created containing black pomegranate peel ethanolic extract at concentrations of 0.5%, 1.0%, and 1.5%. Then, the characteristic properties were determined, the antioxidant power was examined using DPPH (2,2′-diphenyl-1-picrylhydrazyl radical) assay, and the SPF was investigated by the spectrophotometry method. The hydrogel containing extracts of 0.5%, 1.0%, and 1.5% had characteristics that fulfilled the specified requirements, having an inhibition percentage of 27.39%, 54.90%, and 83.91%, respectively, at the sample concentration of 600 ppm, and the SPF of this hydrogel was 2.67, 4.36, and 6.65, respectively. Therefore, according to the findings, hydrogel of black pomegranate peel ethanolic extract is good as a topical antioxidant but is less effective as SPF sunscreen.
Keywords: antioxidant, hydrogel, SPF, black pomegranate peel ethanolic extract
References
[1] Aimin, Wu, Lyn, et al., “Pattern of early human-to-human transmission of Wuhan 2019 novel coronavirus (2019-nCoV), December 2019 to January 2020.,” Euro Surveill. vol. 25, no. 4, p. 2020.
[2] Y.S. Malik, N. Kumar, S. Sircar, and R. Kaushik, “Coronavirus Disease Pandemic (COVID-19): Challenges and a Global Perspective.,” vol. 5812302777, no. May, pp. 1–30, 2020.
[3] T. Carleton, J. Cornetet, P. Huybers, K.C. Meng, and J. Proctor, “Global evidence for ultraviolet radiation decreasing COVID-19 growth rates,” (2020).
[4] M. Wacker and M.F. Holick, “Sunlight and Vitamin D A global perspective for health.,” Dermato-Endocrinology. vol. 5, no. March, pp. 51–108, 2013.
[5] E. Kenneth Weir, T. Thenappan, M. Bhargava, and Y. Chen, “Does Vitamin D deficiency increase the severity of COVID-19?,” Clinical Medicine, Journal of the Royal College of Physicians of London. vol. 20, no. 4, pp. E107–E108, 2020.
[6] Ü. Türsen, B. Türsen, and T. Lotti, “Ultraviolet and COVID-19 pandemic.,” Journal of Cosmetic Dermatology. vol. 19, no. 9, pp. 2162–2164, 2020.
[7] J.D. Orazio, S. Jarrett, A. Amaro-ortiz, and T. Scott, “UV Radiation and the Skin.,” vol. 14, no. 6, pp. 12222–12248, 2013.
[8] M. Reza, S. Ardekani, M. Hajimahmoodi, et al., “Comparative Antioxidant Activity and Total Flavonoid Content of Persian Pomegranate ( Punica granatum L .) Cultivars.,” Iranian Journal of Pharmaceutical Research. vol. 10, no. 3, pp. 519–524, 2011.
[9] M.A. Ebrahimzadeh, R. Enayatifard, M. Khalili, M. Ghaffarloo, M. Saeedi, and J.Y. Charati, “Correlation between sun protection factor and antioxidant activity, phenol and flavonoid contents of some medicinal plants.,”Iranian Journal of Pharmaceutical Research. vol. 13, no. 3, pp. 1041–1048, 2014.
[10] N.A. Peppas, P. Bures, W. Leobandung, and H. Ichikawa, “Hydrogels in pharmaceutical formulations.,” European Journal of Pharmaceutics and Biopharmaceutics. vol. 50, no. 1, pp. 27–46, 2000.
[11] G.R. Bharskar, “A review on hydrogel.,” no. August, p. 2020.
[12] U. Chasanah, “Studies on antioxidant activity of red, white, and black pomegranate (Punica granatum L.) peel extract using DPPH radical scavenging method.,” Farmasains : Jurnal Farmasi dan Ilmu Kesehatan. vol. 5, no. 2, pp. 51–55, 2021.
[13] E. Proksch, “pH in nature, humans and skin,” (2018).
[14] A. Garg, D. Aggarwal, S. Garg, and A.K. Singla, “Spreading of semisolid formulations: An update.,” Pharmaceutical Technology North America. vol. 26, no. 9, pp. 84–105, 2002.
[15] M. Ardana, V. Aeyni, and A. Ibrahim, “Formulasi dan optimasi basis gel hpmc (.,” Journal of Tropical Pharmacy and Chemistry. vol. 3, no. 2, pp. 101–108, 2015.
[16] “summary-1503605.,”
[17] H. Miajlovic, P.G. Fallon, A.D. Irvine, and T.J. Foster, “Effect of filaggrin breakdown products on growth of and protein expression by Staphylococcus aureus.,” Journal of Allergy and Clinical Immunology. vol. 126, no. 6, pp. 1184-1190.e3, 2010.
[18] H. Warnida and Y. Sukawati, “Formulasi Ekstrak Daun Kokang ( Lepisanthes amoena ( Hassk .) Leenh .) dalam Bentuk Gel Anti Acne Formulation of Kokang ( Lepisanthes amoena ( Hassk .) Leenh .) Leaves Extract in Anti-acne Gel Abstract :,” vol. 3, no. 2, p. 2016.
[19] D.K. Baby, Rheology of hydrogels. Elsevier Inc., 2019.
[20] S. Punitha, R. Uvarani, and A. Panneerselvam, “Effect of pH in aqueous (Hydroxy Propyl Methyl Cellulose) polymer solution.,” Results in Materials. vol. 7, no. June, p. 100120, 2020.
[21] S. Ulaen, Y. Banne, and R. Suatan, “PEMBUATAN SALEP ANTI JERAWAT DARI EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb.).,” Jurnal Ilmiah Farmasi Poltekkes Manado. vol. 3, no. 2, p. 96587, 2012.
[22] V. Vučić, M. Grabež, A. Trchounian, and A. Arsić, “Composition and Potential Health Benefits of Pomegranate: A Review.,” Current Pharmaceutical Design. vol. 25, no. 16, pp. 1817–1827, 2019.
[23] Andryani and Viki, “Pemanfaatan Antosianin pada Ubi Jalar Ungu (Ipomoea batatas L.) sebagai Indikator Asam-Basa.,” FMIPA Universitas Negeri Semarang. p. 2015.
[24] M.S. Latha, J. Martis, V. Shobha, et al., “Sunscreening agents: A review.,” Journal of. Clinical and Aesthetic Dermatology. vol. 6, no. 1, pp. 16–26, 2013.
[25] T.E. al Pervaiz, “Naturally Occuring Anthocyanin, Structure, Functions and Biosynthetic Pathway in Fruit Plants.,” Journal of Biochemistry & Physiology. p. 2017.
[26] M. Krause, A. Klit, M. Blomberg Jensen, et al., “Sunscreens: Are they beneficial for health? An overview of endocrine disrupting properties of UV-filters,” (2012).
[27] R.W. Gange, A. Soparkar, E. Matzinger, S.H. Dromgoole, J. Sefton, and R. DeGryse, “Efficacy of a sunscreen containing butyl methoxydibenzoylmethane. against ultraviolet A radiation in photosensitized subjects.,” Journal of the American. Academy of Dermatology. vol. 15, no. 3, pp. 494–499, 1986.
[28] G. Karthikeyan and A.K. Vidya, “Phytochemical Analysis , Antioxidant and Antibacterial Activity of Pomegranate Peel.,” Life Science Informatics Publication. vol. 5, no. 218, pp. 218–231, 2019