Chemical Characterization of Essential Oils With a Biocide Base for Conservation and Restoration

Abstract

Essential oils (EOs) are acclaimed for their antimicrobial properties, leading to their multiple applications in various fields. In this work, four aromatic plants were used, namely thyme (Thymus mastichina L.), everlasting (Helychrysum stoechas Moench), European pennyroyal (Mentha pulegium L.) and fennel (Foeniculum vulgare Mill.). Hydrodistillation was the extraction method used, and the obtained extracts were composed of a variety of volatile molecules, mainly terpenoids and phenylpropenoids. The EO yields were determined, and the chemical composition of these natural products was obtained by gas chromatography-mass spectrometry (GC-MS) analysis. The yields varied greatly in the range of 0.99 to 4.27% (v/w). The two major chemical constituents of the EOs analysed by GC-MS were as follows: thyme – 1,8-cineole and champhor; everlasting – α-pinene and limonene; European pennyroyal – pulegone and isomenthone; fennel – trans-anethole and limonene. This preliminary study is an important contribution to the understanding of EO bioactive compounds that are under investigation to establish their ability to control the biocolonization of cultural heritage.


Keywords: aromatic plants, chemical characterization, essential oils, green biocides

References
[1] Allsopp D. Worldwide wastage: The economics of biodeterioration Applied Microbiology and Biotechnology 2011;38:150–153. https://doi.org/10.1007/s00253- 013-5283-1

[2] Meng H, Katayama Y, Gu JD. More wide occurrence and dominance of ammoniaoxidizing archaea than bacteria at three Angkor sandstone temples of Bayon, Phnom Krom and Wat Athvea in Cambodia. International Biodeterioration & Biodegradation. 2017;117:78–88. https://doi.org/10.1016/j.ibiod.2016.11.012

[3] Sterflinger K, Piñar G. Microbial deterioration of cultural heritage and works of arttilting at windmills. Applied Microbiology and Biotechnology. 2013;97:9637–9646. https://doi.org/10.1007/s00253-013-5283-1

[4] Farooq M, Hassan M, Gull F. Mycobial deterioration of stone monuments of Dharmarajika. Taxila. Journal of Microbiology & Experimentation 2015;2(1):29–33. https://doi.org/10.15406/jmen.2015.02.00036

[5] Liu X, Meng H, Wang Y, Katayama Y, Gu JD. Water is a critical factor in evaluating and assessing microbial colonization and destruction of Angkor sandstone monuments. International Biodeterioration & Biodegradation 2018;133:9– 16. https://doi.org/10.1016/j.ibiod.2018.05.011

[6] Ashraf MA, Ullah S, Ahmad I, Qureshi AK, Balkhair KS, Rehman MA. Green biocides, a promising technology: Current and future applications to industry and industrial processes. Journal of the Science of Food and Agriculture 2014;94:388–403. https://doi.org/10.1002/jsfa.6371

[7] Mateus DMR, Silva RB, Costa FMC, Coroado JPF. Microbiological diversity in the Unfinished Sacristy building of the Convent of Christ, Tomar, and evaluation of its biocide-based control. Conservar Património 2013;17:11-20. https://doi.org/10.14568/cp2012005

[8] Zhang X, Ge Q, Zhu Z, Deng Y, Gu JD. Microbiological community of the Royal Palace in AngkorThom and Beng Mealea of Cambodia by illumina sequencing based on 16S rRNA gene. International Biodeterioration & Biodegradation 2018;134:127–135. https://doi.org/10.1016/j.ibiod.2018.06.018

[9] Céspedes CL, Alarcon JE, Aqueveque P, Seigler DS, Kubo I. In the search for new secondary metabolites with biopesticidal properties. Israel Journal of Plant Sciences 2015;62(4):216–228. https://doi.org/10.1080/07929978.2015.1006424

[10] Jeong SH, Lee HJ, Kim DW, Chung YJ. New biocide for eco-friendly biofilm removal on outdoor stone monuments. International Biodeterioration & Biodegradation 2018;131:19–28. https://doi.org/10.1016/j.ibiod.2017.03.004

[11] Kakakhel MA, Wu F, Gu JD, Feng H, Shah K, Wang W. Controlling biodeterioration of cultural heritage objects with biocides: A review. International Biodeterioration & Biodegradation 2019;143:1047721-1047731. https://doi.org/10.1016/j.ibiod.2019.104721

[12] Palla F, Bruno M, Mercurio F, Tantillo A, Rotolo V. Essential oils as natural biocides in conservation of cultural heritage. Molecules. 2020;25 (730): 1-11. https://doi.org/10.3390/molecules25030730

[13] Casiglia S, Bruno M, Scandolera E, Senatore F, Senatore F. Influence of harvesting time on composition of the essential oil of Thymus capitatus (L.) Hoffmanns. & Link. Growing wild in northern Sicily and its activity on microorganisms affecting historical art crafts. Arabian Journal of Chemistry 2015;12:2704-2712. http://dx.doi.org/10.1016/j.arabjc.2015.05.017

[14] Mishra PK, Shukla R, Singh P, Prakash B, Kedia A, Dubey NK. Antifungal, antiaflatoxigenic, and antioxidant efficacy of Jamrosa essential oil for preservation of herbal raw materials. International Biodeterioration & Biodegradation 2012;74:11-16. http://dx.doi.org./10.1016/j.biod.2012.06.026

[15] Walentowska J, Foksowicz-Flaczyk J. Thyme essential oil for antimicrobial protection of natural textiles. International Biodeterioration & Biodegradation 2013;84:407-411. http://dx.doi.org./10.1016/j.biod.2012.06.028

[16] Pietrzak K, Otlewska A, Danielewicz D et al. Disinfection of archival documents using thyme essential oil, silver nanoparticles misting and low temperature plasma. Journal of Cultural Heritage 2017;24:69-77. http://dx.doi.org/10.1016/j.culher.2016.10.011

[17] Borrego S, Valdés O, Vivar I et al. Essential oils of plants as biocides against microorganisms isolated from Cuban and Argentine documentary heritage. International Scholarly Research Network ISRN Microbiology. 2012; 826786: 1-7. https://doi.org/10.5402/2012/826786

[18] da Cunha PA, Roque OR, Nogueira MT. Plantas aromáticas e óleos essenciais - Composição e aplicações. Lisboa: Fundação Calouste Gulbenkian; 2012.

[19] Veneranda M, Blanco-Zubiaguirre L, Roselli G, Di Girolami G, Castro K, Madariaga JM. Evaluating the exploitability of several essential oils constituents as a novel biological treatment against cultural heritage biocolonization. Microchemical Journal 2018;138:1-6. https://doi.org/10.1016/j.microc.2017.12.019

[20] Fidanza MR, Caneva J. Natural biocides for the conservation of stone cultural heritage: A review. Journal of Cultural Heritage 2019;38:271-286. http://doi.org/10.1016/j.culher.2019.01.005

[21] Mota AS, Martins MR, Arantes S et al. Antimicrobial activity and chemical composition of the essential oils of Portuguese Foeniculum vulgare fruits. Natural Products and Communication 2015;10(4):673–676. https://doi.org/10.1177/1934578X1501000437

[22] Vernin G, Poite JC. GC/MS analysis of volatile components of everlasting (Helichrysum stoechas L.) essential oil. Journal of Essential Oil Research1998;10(5):553-557. https://doi.org/10.1080/10412905.1998.9700969

[23] Rodrigues M, Lopes AC, Vaz F et al. Thymus mastichina: Composition and biological properties with a focus on antimicrobial activity. Pharmaceuticals. 2020;13(479): 1-26. https://doi.org/10.3390/ph13120479

[24] Yasa H, Onar HC, Yusufoglu AS. Chemical composition of the essential oil of Mentha pulegium L. from Bodrum, Turkey. Journal of Essential Oil Bearing Plants 2013;15(6):1040-1043. https://doi.org/10.1080/0972060X.2012.10662609

[25] Ouakouak H, Chohra M, Denane M. Chemical composition, antioxidant activities of the essential oil of Mentha pilegium L., South East of Algeria. International Letters of Natural Sciences 2015;39:49-55. https://doi.org/10.18052/www.scipress.com/ILNS.39.49

[26] Yamini Y, Sefidkon F, Pourmortazavi SM. Comparison of essential oil composition of Iranian fennel (Foeniculum vulgare) obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Flavour and Fragrance Journal 2002;17(5):345- 348. https://doi.org/10.1002/ffj.1117

[27] Teixeira B, Marques A, Ramos C et al. European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Industrial Crops and Products 2012;36:81-87. https://doi.org/10.1016/j.indcrop.2011.08.011