In Vitro Study of Potato (Solanum tuberosum L.) Tolerant to the Drought Stress
In vitro preliminary studies is candidate tolerant of potato (Solanum tuberosum L.) to the drought stress. This study aimed to determine the characters of potato after being exposed to in vitro drought stress conditions using Polyethylene Glycol PEG. This research was conducted at the Tissue Culture Laboratory, UPT Balai Benih Induk Hortikultura Dinas Pertanian Provinsi Sumatera Utara, in Medan and other places in January 2015 until May 2015. This study used Completely Randomized non Factorial Design namely PEG (P) comprising of two levels, namely: P1 = 5 000 mg · L–1, P2 = 6 000 mg · L–1. The results showed that increasing the concentration of PEG resulted in reduction of the percentage of plantlets survival, reduced plantlets height and plantlets dry weight but the increase in the total protein and leaf chlorophyll. Although the banding pattern is relatively the same, there is a brighter visible banding pattern on potato plantlets with OPAA 09 in the range of 65 bp to 75 bp with sequences of GTGGGTGCCA.
 Mexal J, Fisher JT, Osteryoung J, Patrick RCP. Oxygen availability in polyethylene glycol solutions and its implications in plant-water relation. Plant Physiology 1975;55:20–24.
 Mullahey JJ, West SH, Cornell, JA. Effects of simulated drought bypolyethylene glycol on Bahiagrass germination [Online] from http://cat.inist.fr/?aModele =afficheN&cpsidt=3216841. Seed Science and Technology 1996;24:219–224. [[Accessed on March 15th 2016].
 Van Sint Jan V, de Macedo CC, Kinet JM, Bouharmont J. Selection of Al-resistant plants from a sensitive rice cultivar, using somaclonal variation, in vitro and hydroponic cultures. Euphytica 1997;97:303–310.
 Bardakci, F. Random amplified polymorphic DNA (RAPD) markers. Turkish Journal of Biology 2001; 25: 185–196.
 Sirait BA, Roselyna IR, Charloq. Growth and proline content of potato (Solanum tuberosum L.)in vitro candidate tolerant to drought of origin callus. In: Proceedings of the 3rd Annual International Conference Syiah Kuala University (AIC Unsyiah) 2013, in conjunction with the 2nd International Conference on Multidisciplinary Research (ICMR), 2–4 Oct. 2013. Muchlisin ZA, Darmadi, Supradan MD (Eds.). Banda Aceh, Indonesia. p. 366–370.
 Charloq, Panjaitan E, Sirait BA. Study of early screening of potato (Solanum tuberosum L.) as a result of drought stress (in vitro). Paper prented in the 2nd Annual International Conference Syiah Kuala University 2012 & The 8th IMT-GT Uninet Biosciences Conference, 22nd to 24th November 2012, Banda Aceh, Indonesia, p.