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Sirait, B. A., & Charloq, R. (2017). In Vitro Study of Potato (Solanum tuberosum L.) Tolerant to the Drought Stress. KnE Life Sciences, 3(5), 188-192. https://doi.org/10.18502/kls.v3i5.992

https://doi.org/10.18502/kls.v3i5.992

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authors

  • Bilter Anton Sirait
    No author data available.
  • Rosa Charloq

    Rosa Charloq

    Departement of Agriculture, Sumatera Utara University, Jl. Dr. A. Sofian No.3, Padang Bulan, Medan Baru, Kota Medan 20155, Sumatera Utara, Indonesia

Published Date

  • Sep 11, 2017

In Vitro Study of Potato (Solanum tuberosum L.) Tolerant to the Drought Stress

KnE Life Sciences / International Conference on Natural Resources and Life Sciences (NRLS-2016) / Pages 188-192

Abstract

In vitro preliminary studies is candidate tolerant of potato (Solanum tuberosum L.) to the drought stress. This study aimed to determine the characters of potato after being exposed to in vitro drought stress conditions using Polyethylene Glycol PEG.  This research was conducted at the Tissue Culture Laboratory, UPT Balai Benih Induk Hortikultura Dinas Pertanian Provinsi Sumatera Utara, in Medan and other places in January 2015 until May 2015. This study used Completely Randomized non Factorial Design namely PEG (P) comprising of two levels, namely: P1 = 5 000 mg · L–1, P2 = 6 000 mg · L–1.  The results showed that increasing the concentration of PEG resulted in reduction of the percentage of plantlets survival, reduced plantlets height and plantlets dry weight but  the increase in  the total protein and leaf chlorophyll. Although the banding pattern is relatively the same, there is a brighter visible banding pattern on potato plantlets with OPAA 09  in the range of 65 bp to 75 bp with sequences of GTGGGTGCCA.

References
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[5] Sirait BA, Roselyna IR, Charloq. Growth and proline content of potato (Solanum tuberosum L.)in vitro candidate tolerant to drought of origin callus. In: Proceedings of the 3rd Annual International Conference Syiah Kuala University (AIC Unsyiah) 2013, in conjunction with the 2nd International Conference on Multidisciplinary Research (ICMR), 2–4 Oct. 2013. Muchlisin ZA, Darmadi, Supradan MD (Eds.). Banda Aceh, Indonesia. p. 366–370.

[6] Charloq, Panjaitan E, Sirait BA. Study of early screening of potato (Solanum tuberosum L.) as a result of drought stress (in vitro). Paper prented in the 2nd Annual International Conference Syiah Kuala University 2012 & The 8th IMT-GT Uninet Biosciences Conference, 22nd to 24th November 2012, Banda Aceh, Indonesia, p.
403–405