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Harunari, E., Imada, C., Igarashi, Y., Fukuda, T., Terahara, T., & Kobayashi, T. (2015). ISOLATION AND CHARACTERIZATION OF A NOVEL HYALURONIDASE INHIBITOR FROM A MARINE ACTINOMYCETES STRAIN. KnE Life Sciences, 1, 114-119. https://doi.org/10.18502/kls.v1i0.94

https://doi.org/10.18502/kls.v1i0.94

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authors

  • Enjuro Harunari

    Enjuro Harunari

    Graduate School, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477
  • Chiaki Imada

    Chiaki Imada

    Graduate School, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477
  • Yasuhiro Igarashi

    Yasuhiro Igarashi

    Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398
  • Takao Fukuda

    Takao Fukuda

    Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama 939-0398
  • Takeshi Terahara

    Takeshi Terahara

    Graduate School, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477
  • Takeshi Kobayashi

    Takeshi Kobayashi

    Graduate School, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477

Published Date

  • Feb 1, 2015

ISOLATION AND CHARACTERIZATION OF A NOVEL HYALURONIDASE INHIBITOR FROM A MARINE ACTINOMYCETES STRAIN

KnE Life Sciences / International Symposium on Aquatic Product Processing (ISAPPROSH) 2013 / Pages 114-119

Abstract

A novel hyaluronidase inhibitor (HI) was isolated from the culture extract of a marine- derived actinomycete strain. This strain MB-PO13 was isolated from ascidian (Molgula manhattensis) in Tokyo Bay. Out of about 1,000 isolates from various marine organisms, strain MB-PO13 had the strongest inhibitory activity and was selected for further study. The strain showed abundant-to-moderate growth on most media, forming a grayish mycelium. On the basis of the taxonomical characteristics, the strain was classified as belonging to the genus of Streptomyces and was named as Streptomyces sp. strain MB-PO13. The structure of HI was elucidated by interpretation of NMR data. HI displayed about 25-fold potent hyaluronidase inhibitory activity against hyaluronidase than glycyrrhizin.

Keywords: marine actinomycetes; Streptomyces; hyaluronidase inhibitor 

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