EFFECT OF PREPARING CONDITIONS ON PROPERTIES OF GELATIN FILM FROM HORSE MACKEREL SCALE

Abstract

In this study, the effect of preparing conditions, such as protein concentrations and glycerol concentrations of film forming solution (FFS) on properties of gelatin film from horse mackerel scale were investigated. Properties of edible film from gelatin was extracted at 70oC for 1 h at various protein concentrations (1, 2, and 3%) in film-forming solution (FFS) were examined.. Films from FFS containing higher protein content showed the higher TS and EAB. WVP of film increased with increasing protein concentration of FFS. The study about the effect of glycerol in FFS on the film property showed that addition of glycerol as a plasticizer into FFS at the concentrations of 0, 10, 15, 20 and 25 % of protein lead to the decline of TS of gelatin film, while both EAB and WVP of film increased. The SDS-PAGE patterns showed that all gelatin films consisted of two different α chains, α1 and α2, as well as a β component and no differences in protein patterns among the protein concentrations and glycerol concentrations of FFS.

Keywords: gelatin, films, horse mackerel, scales.

References
ASTM. 1983. In Annual book of ASTM standards.
Gómez-Guillén MC., Pérez-Mateos M, Gómez-Estaca J, López-Caballero E.,
Giménez B, Montero P. 2008. Fish gelatin: A renewable material for developing active biodegradable films. Trends in Food Science & Technology, , 1-14.
Gontard N, Guilbert S, Cuq JL. 1992. Edible wheat gluten films: influence of the main process variables on film properties using response surface methodology. Journal of Food Science, 57(1), 190-195.
Kittiphattanabawon P, Benjakul S., Visessaguan W, Nagai T, Tanaka M. 2005. Characterisation of acid soluble collagen from skin and bone of bigeye snapper (Priacanthus tayenus). Food Chemistry, 89, 363-372.
Limpisophon K, Tanaka M., Weng W, Abe S, Osako K. 2009. Characterization of gelatin films prepared from under-utilized blue shark (Prionace glauca) skin. Food Hydrocolloids, 23, 1993-2000.
Lowry OH, Rosebrough NJ, Farr AL., Randall RJ. 1951. Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193, 256-275.
Shimizu U. 1987. Neriseihin In Suyama, M., & Kounosu, S. (eds), Suisanshokuhingaku (pp. 262-264). Tokyo: Kouseisha kouseikaku (in Japanese).
Yamanaka H, Tanaka M. 2007. Suisanbutsu no riyou. (2nd ed.) (pp. 18-28). Tokyo: Seizando shoten (in Japanese).