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Nova Handayani, R., Yunus, F., Ibrahim, E., & Rengganis, I. (2020). The Balance between Interleukin 5 and Interleukin 10 Level in Persistent Atopic Asthma after House Dust Mite Allergen Dermatophaigoides pteronyssinus (Der p) Stimulation. KnE Life Sciences, 5(2). https://doi.org/10.18502/kls.v5i2.6434

https://doi.org/10.18502/kls.v5i2.6434

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authors

  • Rahmaya Nova Handayani

    Rahmaya Nova Handayani

    Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
  • Faisal Yunus

    Faisal Yunus

    Department of Pulmonology and Respiratory Medicine, Medicine Faculty of Indonesia University, Persahabatan Hospital, Jakarta, Indonesia
  • Ermita Ibrahim

    Ermita Ibrahim

    Department of Medical Physiology, Medicine Faculty of Indonesia University, Jakarta, Indonesia
  • Iris Rengganis

    Iris Rengganis

    Department of Internal Medicine, Faculty of Medicine, Indonesia University, Dr.Cipto Mangunkusumo Hospital, Jakarta, Indonesia

Published Date

  • Feb 11, 2020

The Balance between Interleukin 5 and Interleukin 10 Level in Persistent Atopic Asthma after House Dust Mite Allergen Dermatophaigoides pteronyssinus (Der p) Stimulation

KnE Life Sciences / The 2019 International Conference on Biotechnology and Life Sciences (IC-BIOLIS) /

Abstract

Asthma is a chronic inflammation of the respiratory tract characterized by coughing, shortness of breath, wheezing and limited expiratory airflow. Interleukin 10 (IL-10) is a cytokine produced by T regulators which functions to suppress pro-inflammatory production and development. Interleukin 5 (IL-5) is glycoprotein produced by Th2 which acts as a proinflammatory agent. This study aimed to analyze how to balance between IL-10 and IL-5 levels on persistent atopic asthma. The study design was an experimental research method with invitro study used isolation and cell culture of Peripheral Blood Mononuclear Cell (PBMC) with negative control Roswell Park Memorial Institute (RPMI) medium, positive control Phytohaemaglutinin (PHA) and house dust mite allergen stimulation 10 µl. This study involved 39 samples asthmatic. There was a significant increase in IL-5 levels after stimulation of house dust mite allergens with p: 0.000; there was a significant decrease in IL-10 levels after house dust mite allergen stimulation with p: 0.000. Our study suggests that IL-5 and IL-10 balance is related to persistent atopic asthma after house dust mite allergen Der p stimulation.


Keywords: Interleukin 5, Interleukin 10, Atopic Asthma, house dust mite allergen, ELISA

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