Preserving biodiversity is a global problem that was reflected in the Convention on Biological Diversity which appeared in 1992. The adoption of the Convention was an answer to the intensified problem of a significant reduction in the diversity of genetic resources. Botanical gardens are the main centres for preserving the biodiversity of plants that solve the problem of preserving the gene pool. One of the main tasks of botanical gardens is to monitor plant populations in their natural habitats in situ. This paper describes the monitoring of the current state of peonies and sedges in the Semistozhki area of the Andropov district in the Stavropol region. The research determines the growing locations and the belongingness of species to plant associations in the vegetation variety of the Stavropol region. It also determines the species that may be treated as rare due to their limited distribution area. The territory is marked by the presence of Paeonia tenuifolia L., which is a tertiary relict, and Paeonia biebersteiniana Rupr., which is subendimic for Stavropol region. Rear species with low numbers that are not threatened by grave danger of extinction are also present. Carex hordeistichos Vill. and c. otrubae Podp. species in the area are small in number. Their populations in the area are inadequate with young, vegetative and strong specimens lacking. The limiting factors are represented by human agricultural activities. Several specimens of a narrow-leaved peony of pink colour and a big number of petals were planted on an experimental stretch of the botanical garden to continue the analysis ex-situ.