State of the Hemostatic System in the Newborn Calves with Diverse Birth Weights


The studies on the peculiarities of hemostatic indices in calves (n=250) of Holstein- Friesian breed with diverse birth weights were conducted under the conditions of a dairy farm with the aid of generally accepted clinical, instrumental and laboratory methods. The research has demonstrated that in comparison with the adults the newborns weighing from 36.5 to 29 kg revealed a diversity of hemostatic indices but generally there was a functionally acceptable balance of its links without a tendency to coagulopathy or thrombosis. Fetal growth restriction in cattle with low birth weight has a significant effect on the hemostatic system. Insufficient weight of more than 7 % provokes hypercoagulability syndrome which is caused by increased levels of adrenalin, toxic products of impaired metabolism and destruction of erythrocyte membranes. The newborns with lower weight demonstrated signs of primary coagulopathy conditioned by functional inferiority of the organs in which the synthesis of coagulation factors occurred that led to the development of disseminated intravascular coagulation. The revealed dependence of the hemostatic disorders evidence on the severity of weight insufficiency indicates coagulopathy, emerging in epigenetics of the main pathology after primary damage and conditioning the severity of the newborns’ state, as one of the main mechanisms of hypotrophy pathogenesis.

[1] Medvedev, I.N., Zavalishina, S.Yu. (2014). Age dynamics of hemostatic vascular activity in calves during early ontogenesis. Veterinary medicine, vol. 2, pp. 46–49.

[2] Sola-Visner, M. (2012). Platelets in the neonatal period: developmental differences in platelet production, function, and hemostasis and the potential impact of therapies.Hematology Am Soc Hematol Educ Program 2012, pp. 506–511.

[3] Toulon, P. (2016). Developmental hemostasis: laboratory and clinical implications. Int. Journal Lab, Hematol, no. 38, pp. 66–77.

[4] Koltsova, E.M., Balashova, E.N., Panteleev, M.A. et al. (2018). Laboratory aspects of the hemostasis in the newborns. The Issues of Hematology. Oncology and Immunopathology in Pediatrics, vol. 17(4), pp. 100–113.

[5] Saxonhouse, M.A., Manco-Johnson, M.J. (2009). The Evoluation and Management of Neonatal Coagulation Disorders. Semin Perinatol, vol. 33(1), pp. 52–65.

[6] Manco Johnson, M.J. (2005). Development of hemostasis in the fetus. Thrombosis Research, vol. 115(1), pp. 55–63.

[7] Khayrullin, I.N., Shishkov, N.K., Kazimir, A.N. et al. (2012). Calves hypotrophy. Materials of the IVth International Scientific and Practical Conference ``Actual issues of veterinary medicine, biology and ecology''. Ulyanovsk, vol. 1, pp. 228–231.

[8] Shitikova, A.S. (2008). Thrombocytopathies, congenital and acquired. St. Petersburg: Information & publishing centre ``Military medical Academy''.

[9] Piccione, G., Casella, S., Pennisi, P. et al. (2010). Monitoring of physiological and blood parameters during perinatal and neonatal period in calves. Arq. Bras. Med. Ve.t Zootec., vol. 62, pp. 1–12.

[10] Zhmudyak, M.L., Povalikhin, A.N., Strebukov, A.V. et al. (2006). Diagnosis of diseases by the methods of probability theory. Barnaul: Publishing house AltSTU.

[11] Barkagan, Z.S., Momot, A.P. (2008). Diagnosis and controlled therapy of hemostatic disorders. Moscow: Newdiamed.

[12] Alekhin, Yu.N. (2013). Diagnostic methods for perinatal pathology in cattle: methodological guide. Voronezh.

[13] Togaybaev, A.A., Kurguzkin, A.V., Rikun, I.V. (1988). Method for the diagnosis of endogenous intoxication. Laboratory work, no. 9, pp. 22–24.

[14] Tonkoshkurova, O.A., Dmitriev, A.I., Dmitrieva, R.E. (1996). Determining the concentration of extra-erythrocyte hemoglobin in plasma (serum) by the hemoglobin cyanide method. Clinical laboratory diagnosis, no. 2, pp. 21–22.

[15] Shakhov, A.G., Alekhin, Yu.N., Shabunin, S.V. et al. (2013). Methodological manual for the diagnosis and prophylaxis of antenatal and intranatal origin disorders in calves. Voronezh: Publishing House ``Istoki''. [16] Belova, T.A., Zavalishina, S.Yu., Nagornaya, O.V., Medvedev, I.N. (2014). The ability to aggregate erythrocytes and platelets in young cattle during the first 10 days of life. Bulletin of Peoples' Friendship University of Russia. Series: Ecology and Life Safety, no. 2, pp. 36--41.

[17] Toulon, P. (2016). Developmental hemostasis: laboratory and clinical implications. Int. Journal Lab. Hematol, no. 38, pp. 66–77.

[18] Yum, S.K., Moon, C.-J., Youn, Y.-A. et al. (2016). Risk factor profile of massive pulmonary haemorrhage in neonates: the impact on survival studied in a tertiary care centre. Journal Matern. Fetal. Neonatal. Med., no. 29, pp. 338–343.

[19] Saxonhouse, M.A., Manco-Johnson, M.J. (2009). The evaluation and management of neonatal coagulation disorders. Semin. Perinatol., no. 33, pp. 52–65.

[20] Gundorov, M.A, Pakhmutov, I.A., Glavinskiy, A.V. (2013). Oxidative stress and endogenous intoxication under diarrhea in the newborn calves with hypotrophy. Scientific notes of the Kazan State Academy of Veterinary Medicine named after N.E. Bauman, vol. 215, pp. 85–90.

[21] Kuznik, B.I., Mishchenko, V.P. (1971). Effect of adrenalin on coagulability of the blood and lymph. Bulletin of Experimental Biology and Medicine, no. 72(5), pp. 1245–1247.

[22] Fogelson, A.L., Hussain, Y.H., Leiderman, K. (2012). Blood clot formation under flow: the importance of factor XI depends strongly on platelet count. Biophysical Journal, no. 102(1), pp. 10–18.

[23] Horsti, J. (2002). Prothrombin time: evaluation of determination methods. Tampere University Press.

[24] Marder, V.J., Aird, W.C., Bennett, J.S. et al. (2013). Hemostasis and Thrombosis: Basic Principles and Clinical Practice, 6th ed. Philadelphia: Lippincott Williams & Wilkins.

[25] Kostousov, V., Nguyen, K., Hundalani, S.G. et al. (2014). The influence of free hemoglobin and bilirubin on heparin monitoring by activated partial thromboplastin time and anti-Xa assay. Arch. Pathol. Lab. Med., no. 138, pp. 1503–1506.

[26] Wada, H. (2004). Disseminated intravascular coagulation. Clin Chim Acta, vol. 344(1–2), pp. 13–21.