Impact of Centella Asiatica Extract on Memory and Adrenal Weight after Chronic Stress on Spraque Dawley rats


Chronic stress is a common daily problem which may lead to the disruption of brain function including memory. One of the treatments for stress-related problems is the administration of medicinal plants such as Centella asiatica (CA). The objective of this research is to investigate the effects of the extract of Centella asiatica on adrenal weight and memory after chronic stress. Thirty adult male Spraque-Dawley rats (Rattus norvegicus), were divided into 6 groups; each group consisted of 5 rats (n=5). The control group (C) was as normal control without stress and Treatment groups were Stress, CA150, CA300, CA600 and Fluo10 that receiving chronic immobilization stress. Stress group only received chronic stress treatment while other groups were also
treated with CA ethanolic extract at 150, 300, and 600 mg/kgBW and 10 mg/kgBW of fluoxetine respectively. Chronic stress was triggered by immobilizing the rats in an acrylic tube for 6 hours per day for 21 days. Following this, the Moris Water Maze test was performed for 6 days to test the memory. One day after the test, the rats were terminated; the adrenal glands were evacuated and weighed. Acquisition trial showed improvement in memory performance from day to day (p<0.05) but there was no difference between groups (p > 0.05). Probe trial revealed the same result (p>0.05). The average percentage of adrenal glands relative to body weight in group Control, Stress, Stress, CA150, CA300, CA600 and Fluo10 were: 0.1592, 0.1838, 0.1942, 0.167, 0.1774 and 0.2024%, respectively (p>0.05). We conclude that the CA extract might influence memory performance and adrenal weight after chronic stress exposure although it was not statistically significant.

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