PROLIFERATION INHIBITORY ACTIVITY OF THE ACTIVE FRACTION MARINE SPONGE <i>Cinachyrella</i> sp. AGAINST CELL LINE T47D
Marine sponges Cinachyrella sp. (Family:Tetillidae) in Kukup beach, Kemadang Village, Tanjungsari District, Gunung Kidul, DIY were producing diversity secondary metabolites such as polyketides, alkaloids, peptide and terpene. The purpose of this study was investigated proliferation inhibitory activity of active fraction Cinachyrella sp. against cell line T47D. Sponges samples were collected manually from rocky substrate at depth 0.5 m. The sponges was minced and extracted with 95% ethanol. The ethanol extract was partitioned sequentially with ethyl acetate. The extract ethyl acetate was fractionation with 4 organic solvent, in increasing order of polarity with vacuum liquid chromatography column (VLC) method. Doubling time method was applied to analyse the inhibition proliferative cell line T47D. Resulted showed ethyl acetate extract of Cinachyrella sp. were 12 fractions and all tested fraction obtained by thin layer chromatography (TLC). Fractions that have the same value Rf grouped together to obtain 6 fractions. The fraction number 5 exhibited proliferation inhibitory activity to cell line T47D. The Rf value of active fraction number 5 were 0.125; 0.25 and 0.437. The active fraction 5 than isolation by preparative thin-layer chromatography (PTLC) was 5 isolate fractions preparative. The isolate fractions preparative number 5 exhibited proliferation inhibitory activity against cell line T47D. Fraction which determined by cerium sulfate and results was expressed terpene and alkaloid.
Keywords: Cinachyrella sp., Doubling time method, proliferation inhibitory activity.
Bell, J. J. 2008. Review The functional roles of marine sponge. J. Estuarine, Coastal and Shelf Scienc. Vol. 79. Pp: 341-353.
Brackman, J. C., and D. Doloze. 1986. Chemical defence in sponges. Pure & Appl. Chem. Vol 58 93): 357-364.
Gewirtz. 1999. Apo Review Introduction to Apoptosis.
Iwamaru, A., E. Iwado, and S. Kondo. 2007. Eupalmarea acetate, a novel anticancer agents from Caribbean gorgonian octocorals, induces apoptosis in malignant glioma cells via the c-jun NH2-terminal kinase pathway. Molecular Cancer Therapeutics. 6:184-192.
Jennei, R.I., and E. Meiyanto. 2009. Co-application of chemotherapy ethyl acetate fraction ethanolic leaf extract of continued life (Gynura procumbens (Lour) Merr on kanker payudara MCF-7 cells. Magazine Pharmaceutical Sciences. Vol. VI, No.3: 132-141.
Larghi, E., B. Obrist, and T. Kaufman. 2008. “A Formal Total Synthesis of The Marine Alkaloid aaptamine”. Tetrahedron. Volume 64, Issue 22
Muller, W., G.L.P. Grebenjuk, H.C. Schroder, F. Brummer, U. Hentschel, I.M. Muller, H.J. Breter. 2004. Review. Sustainable Production of Bioactive Compounds by Sponges-Cell Culture and Gene Cluster Approach: A Review Marine Biotechnology. 6: 105-117.
Oku, N., K. Takada, R.W. Fuller, J.A. Wilson, M.L. Peach, L.K. Pannell, J.B. McMohan, and K.R. Gustafson. 2010. Isolation, Structural Elucidation and Absolute Stereochemistry of Enigmazole A, a Cytotoxic Phosphomacrolide from the Papua New Guiena Marine Sponge Cinachyrella enigmatica. Journal American Sociality, 132: 10278-10285.
Shimagawa, H., S. Kuribayashi, T. Teruya, K. Suenaga, and H. Kigoshi. 2006. Cinachyramine, the novel alkaloid possessing a hidrazone and two aminals from Cinachyramine sp. Tetrahedron Letters, 47:1409-1411.
Nurhayati, A.P.D., R. Pratiwi, B. Wahyuono, Istriyati. 2011, Anticancer studies with Cellular Approaches And Sea Sponge Conservation Efforts In Tanjungsari Cinachyrella sp, Gunung Kidul. Research grant program berbasis EfSD IMHERE Faculty of Biology, Gadjah Mada University.
Nurhayati, A.P.D., R. Pratiwi, B. Wahyuono, Istriyati. 2012, Anticancer activity Cinachyrella sp Sea Sponge In Tanjungsari, Gunung Kidul. Doctoral grant. ITS. Palozza, T.B.P., S. Serini, N. Maggiano, P. Giuseppe, and F.O. Ranelletti. 2005. Steady-State and Heregulin-a-Induced COX-2 Pathways in Colon Cancer Cells, J.Nutr. 135:129136.
Perkasa, D.P., and A. Budiyanto. 2010. Isolation and antibacterial activity of sponge Cinachyrella sp. Laporan Penelitian Center for the Application of Isotope and Radiation Technology.
Schupp, P. J., C.K. Schupp, S. Whitefield, A. Engemann, S. Rohde, T. Heimsceidet, J. Pezutto, T.P. Kondratiuk, E.J. Park, L. Marler, B. Rostom, and A.D. Wright. 2009. Cancer Chemopreventive and Anticancer Evaluation of Extracs and Fractions from Marine Macro and Micro-organism Collected from Twilight zone Waters Around Guam. Nat. Prod. Commun. 4(12): 1717-1728.
Tjindarbumi, D., and R. Mangunkusumo. 2002. Cancer in Indonesia, Present and Future. Japan Journal of Clinical Oncology. 32 (supplement): 17-21.
Ullah, M.F., and M. Aatif. 2009. Hot Topic. The footprints of cancer development : cancer biomarker. Cancer Treatment Reviews. 35: 193-200.
Watanadilok, R., P. Sonchaeng, A. Kijjoa, A.M. Damas, L. Gales, A.M.S. Silva, and W. Herz. 2001. Tetillapyrone and Nortetitllapyron, Two Unusual Hydroxipyan 2-ones from the Marine Sponge Cinachyrella japonica. J. Nat. Prod. 64: 1056-1058.