STUDY OF PROPOXUR DEGRADING BACTERIA ISOLATED FROM VARIOUS SAMPLING SITE OF RICE FIELD FROM NGAWI
Abstract
Propoxur (2-isopropoxyphenyl-N-methylcarbamate) is a carbamateinsecticide which has negative impact on the environment. Propoxur is readily degraded by soil microorganism in most soils. Increased enviromental degradation of propoxur constitute the result of increase in activities of soil pesticide-degrading bacteria. The aims of the present study are to isolate andscreen the bacteria with high potential ability to degradepropoxur from soil samples. Isolation and selection were carried out by enrichment cultures technique.Propoxur degrading bacteria were isolated from 5 soil samples in different locations of rice field from Ngawi. The biodegradation ofpropoxur by isolated bacteria were analysed by High Performance Liquid Chromatography (HPLC). Ten consorsia were selected and purified. One isolate was able to use propoxur at 3000 ppm as sole source of carbonfor the growth. The result suggested that H2-NG isolate hydrolysedpropoxur with the yield of 2-isopropoxyphenol and methylamine.
Keywords: Ngawi, Isolation, Propoxur, Biodegradation, Bacteria
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