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Trofimov, A. G., & Vershinina, S. D. (2018). About the Applicability of Bone and Cartilage Acid-free Staining for Anura. KnE Life Sciences, 4(3), 156–160. https://doi.org/10.18502/kls.v4i3.2118

https://doi.org/10.18502/kls.v4i3.2118

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authors

  • A G Trofimov
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  • S D Vershinina
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Published Date

  • May 3, 2018

About the Applicability of Bone and Cartilage Acid-free Staining for Anura

KnE Life Sciences / The Second International Conference on Amphibian and Reptiles Anomalies and Pathology / Pages 156–160

Abstract

The article describes the first use of acid-free alcian-alizarin staining for tailless amphibians in comparison with the standard method of acid staining on the example of the moor frog. The acid-free protocol is applicable for Anura and it has a number of advantages, such as the absence of decalcification, safety for the researcher and
others. However, this method also has limitations, such as the binding of alcian blue with soft tissues.

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