THE POTENCY OF LOCAL ISOLATES FROM NORTH SUMATERA IN THE DEGRADATION OF NAPHTALENE
The potency of biosurfactant–producing bacteria isolated from oil-contaminated coast, Belawan North Sumatera, in the degradation of hydrocarbon compound such as naphthalene has been studied. Thirteen isolates has been obtained using Bushnell Haas Agar containing 2% naphthalene as the sole carbon source. The bacterial growth was observed using Standard Plate Count method. The ability of each isolates to produce bioactive-surface compound was determined. A drop-collapsing test was performed to examine the activity of each biosurfactant. All isolates tested grew well in Bushnell Haas Broth medium. Among those, two isolates were able to remove almost all (99%) naphthalene from the medium during 15 days. Those two isolates have also shown highest concentration of biosurfactant production which is 75.180 ppm.
Keywords: local isolates, biosurfactant, naphthalene degradation.
Abraham, T.J., S.A. Shanmugam, A. Uma, R. Palaniappan, and K. Dhevendaran. 2004. Biocontrol of shrimp bacterial pathogenic using Penaeid larva associated bacterium Alteromonas sp. J. Aqua. Trop. 16(1):11-12
Bertrand, J.C., P. Bonin, M. Goutx, M. Gauthier, and G. Mile. 1994. The Potential application of biosurfactant in combating hydrocarbon polution in marene environment. Res. Microbiol. 145: 53 – 56
Chandrasekaran, E.V. and J.N. BeMiller. Constituent analysis of glucose aminoglycans p. 89-96 In: R.L.Whistler (ed). Methods in Carbohydrate Chemistry, Academic Press, Inc. New York.
Fachrudin. 2004. Dampak tumpahan minyak pada biota laut. http://cdc.eng.ui.ac.id/ article/ articleview/1078/1/25 (accessed on: 20 May 2005)
Gautam, K.K. and V.K. Tyagi. 2006. Microbial surfactants: A Review. J. Oleo Sci. 55 (4):155166
Hedlund, B.P., A.D. Geiselbrecht, J.T. Staley. 2001. Marinobacter strain NCE312 has a Pseudomonas-like naphthalene dioxygenase. FEMS Microbiol. Lett. 201, 47-51.417.
Jain, D.K., D.L.C. Thompson, H. Lee, and J.T. Trevors. 1991. A Drop collapsing Testfor screening surfactant producing microorganism. J. Microbiol. Methods. 13:271-279
Johnsen, A.R., L.Y. Wick, H. Harms. 2005. Principles of microbial PAH degradation in soil. Environmental Pollution 133: 71–84.
Kang, H., S.Y. Hwang, Y.M. Kim, E. Kim, Y.S. Kim, S.K. Kim. 2003. Degradation of phenanthrene and naphthalene by a Burkholderia species strain. Can. J. Microbiol. 49: 39-144.
Lay, B.W. 1994. Analisis Mikroba di Laboratorium. Penerbit PT. Radja Grafindo Perkasa, Jakarta.
Parra, J.L., J. Guinea, M.A. Manresa, M. Robert, M.E. Mercade, F. Comelles, and M.P. Bosch. 1989. Chemical characterization and Physicochemical behavior of biosurfactant. J. Am. Oil Chem. Soc. 66:141-145
Pumphrey, G.M., and E.L. Madsen. 2007. Naphthalene metabolism and growth inhibition by naphthalene in Polaromonas naphthalenivorans strain CJ2. Microbiology 153: 37303738.