DEVELOPMENT OF DNA BIOSENSOR BASED ON SILVER NANOPARTICLES UV-Vis ABSORPTION SPECTRA FOR <i>Escherichia coli</i> DETECTION
In this research we reported the synthesis of oligonucleotide-silver nanoparticle (OSN) conjugates and demonstrated their use along with magnetic beads as biosensor for Escherichia coli detection under magnetic field condition. Oligonucleotide DNA probes were conjugated on silver nanoparticles using alkanethiols linker. Two kinds of alkanethiols linker, 11-mercaptoundodecanoic acid (11-MUDA) and 16-mercaptophexadecanoic acid (16-MHDA) were compared to get the best probe conjugation yield and OSN UV-Vis absorption spectra properties. Three different methods of Escherichia coli DNA isolation i.e. Chen and Kuo (1993), Phenol Chloroform Isoamylalcohol (PCI) extraction and boiling lysis were also compared to explore the performance of the biosensor towards the DNA target purity. Detection process through hybridization between the DNA probe and the target was carried out at 55oC for 1 hour incubation time. The results showed that 16-MHDA gave higher conjugation yield and higher OSN UV-Vis absorption spectra than 11-MUDA. The biosensor was able to detect the presence of the DNA target which was isolated from the three isolation methods. The best detection signal was achieved by Chen and Kuo isolation method in which it could detect the presence of the DNA target up to 1.3 ng/µL.
Keywords: DNA biosensor, Silver Nanoparticles, Escherichia coli
Arora, P., A. Sindhu, N. Dilbaghi, and A. Chaudhury. 2011. Biosensors as innovative tools for the detection of food borne pathogens. Biosensor Bioeletron. vol. 28. 11-12
Chang, H., L. Tang, Y. Wang, J. Jiang, and J. Li. 2010. Graphene fluorescence resonance energy transfer aptasensor for the thrombin detection. Anal. Chem. vol. 82. 2341–2346
Chen, W.P., and T.T. Kuo. 1993. A simple and rapid method for preparation of gram-negative bacterial genomic DNA. Nucleic Acids Research. vol 21. 2260.
Graham, D.L., H.A. Ferreira, N. Feliciano, P.P. Freitas, L.A. Clarke, and M.D. Amaral. 2005. Magnetic field-assisted DNA hybridisation and simultaneous detection using micron-sized spin-valve sensors and magnetic nanoparticles. Sensors and Actuators B: Chemical. vol.107 (2). 936-944. doi:10.1016/j.snb.2004.12.071
Gupta, R.K., M.P. Srinivasan, and R. Dharmarajan. 2012. Synthesis of 16-Mercaptohexadecanoic acid capped gold nanoparticles and their immobilization on a substrate. Mater Lett. vol.67. 315–319
Guzmán, M.G., J. Dille, and S. Godet. 2009. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Intern J. Chem Biol Engineering. vol. 2 (3), 104-111.
Henglein, A., and M. Giersig. 1999. Formation of Colloidal Silver Nanoparticles: Capping Action of Citrate. J. Phys. Chem. B 1999, 103, 9533-9539
Huo, Q. 2007. A perspective on bioconjugated nanoparticles and quantum dots. Colloids and Surfaces B: Biointerfaces. vol.59.1–10
Ivnitski, D., I. Abdel-Hamid, P. Atanasov, and E. Wilkins E. 1999. Biosensors for detection of pathogenic bacteria. Biosens Bioelectron. vol.14. 599–624
Jack, B. 2010. Chemical Synthesis of Silver Nanoparticles for Nanoparticles for Light Trapping Applications In Silicon Solar Cells.
Kouassi, G.K., and J. Irudayaraj. 2006. Magnetic and Gold-Coated Magnetic Nanoparticles as a DNA Sensor. Anal. Chem. vol.78 (10). 3234–3241. DOI: 10.1021/ac051621j
Leonard, P., S. Hearty, J. Brennan, L. Dunnea, J. Quinn, T. Chakraborty, and R. O’Kennedy. 2003. Advances in biosensors for detection of pathogens in food and water. Enzyme Microb Tech. vol.32. 3–13
Li, G., V. Joshi, R.L. White, S.X. Wang, J.T. Kemp, C. Webb, R.W. Davis, and S. Sun. 2003. Detection of single micron-sized magnetic bead and magnetic nanoparticles using spin valve sensors for biological applications. J. Appl. Phys. vol.93. 7557.doi:10.1063/1. 1540176
Mao, X., L. Yang, X. Su, and Y. Li. 2006. A nanoparticle ampliûcation based quartz crystal microbalance DNA sensor for detection of Escherichia coli O157:H7. Biosensors and Bioelectron. vol 21. 1178–1185
Nair, N., N. Wentzel, and A. Jayaraman.2011. Effects of polydispersity on Potential of Mean Force between Functionalized Nanoparticles in a Homopolymer Mat rix: Self-Consistent PRISM Theory-Monte Carlo Simulation Study’J. Chem Phys. vol. 134, 194906
Šileikaite, A., I. Prosycevas, J. Puiso, A. Juraitis, and Guobiene. 2006. Analysis of Silver Nanoparticles Produced by Chemical Reduction of Silver Salt Solution. Mater Sci. vol. 12 (4). 287-291.
Solomon, S.D., M. Bahadory, A.V. Jeyarajasingam, S.A. Rutkowsky, C. Boritz, and L. Mulfinger. 2007. Synthesis and Study of Silver Nanoparticles. J Chem Edu, 322-325.
Sumar, A. Kumar. 2008. Recent Advances in DNA Biosensor. Sensors Transducers J. vol. 92 (5). 122-133
Thompson, D.G., A. Enright, K. Faulds, W.E. Smith, and D. Graham. 2008. Ultrasensitive DNA Detection Using Oligonucleotide-Silver Nanoparticle Conjugates. Anal. Chem. vol. 80. 2805-2810
Vogt, R.L., and L. Dippold. 2005. Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002. Public Health Rep. vol.120 (2). 174–8.
Wang, J. 2003. Nanoparticle-based electrochemical DNA detection. Analytica Chimica Acta. vol. 500. 247–257
Xu, K., J. Huang, Z. Ye, Y. Ying, and Y. Li. 2009, Recent Development of Nano-Materials Used in DNA Biosensors. Sensors, vol. 9. 5534-5557; doi:10.3390/s90705534
Zhu, N., A. Zhang, P. He, and Y. Fang. 2004. DNA Hybridization at Magnetic Nanoparticle with Electrochemical Stripping Detection. Electroanalysis. vol.16 (23). 1925-1930. DOI:10.1002/elan.200303028.