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Rahmawati, R. R., Kusumawati, E., Aninda, L. P., Mushlih, M., Gofur, A., & Listyorini, D. (2015). IDENTIFICATION OF JAVAN LANGUR (<i>Trachypithecus auratus</i&gt;) IN JAVAN LANGUR CENTER (JLC) COBAN TALUN, BATU BASED ON Cytochrome-b SEQUENCE. KnE Life Sciences, 2(1), 274-277. https://doi.org/10.18502/kls.v2i1.157

https://doi.org/10.18502/kls.v2i1.157

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authors

  • Rita Rizky Rahmawati

    Rita Rizky Rahmawati

    Department of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang
  • Emy Kusumawati

    Emy Kusumawati

    Department of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang
  • Lulus Putri Aninda

    Lulus Putri Aninda

    Department of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang
  • Miftahul Mushlih

    Miftahul Mushlih

    Faculty of Biology, Gadjah Mada University, Yogyakarta
  • Abdul Gofur

    Abdul Gofur

    Department of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang
  • Dwi Listyorini

    Dwi Listyorini

    Department of Biology, Faculty of Mathematic and Natural Science State University of Malang, Jl. Semarang 5, Malang

Published Date

  • Sep 20, 2015

IDENTIFICATION OF JAVAN LANGUR (<i>Trachypithecus auratus</i>) IN JAVAN LANGUR CENTER (JLC) COBAN TALUN, BATU BASED ON Cytochrome-b SEQUENCE

KnE Life Sciences / International Conference on Biological Sciences (ICBS-2013) / Pages 274-277

Abstract

Javan Langur (Trachypithecus auratus) is an endemic langur in Indonesia which classified as vulnerable primate. Some researches divided Javan Langur species into some subspecies, yet was not described properly. Cytochrome-b sequence from mitochondrial DNA is able to determine samples between and intra-species as well. This study aimed to identify the Javan Langur (Trachypithecus auratus) samples named Andin (@&) and Bobby (B&), both are rehabilitated in Javan Langur Center (JLC). The amplification of Cytochrome-b gene from whole blood DNA with PCR technique using forward primer L151625’­CTTCCATGAGGACAAATATC-3’ and reverse primer Rmuc15’-GTGGAGTATAGGTATGATTGC-3’. The phylogenetic tree reconstruction based on MEGA 5 using maximum likelihood (ML) method and pairwise distance analysis with Kimura-2 parameter model resulted that Andin and Bobby are in the same group with
T. a. auratus. Andin is in the same clade with T. a. auratus haplotype aaF, while Bobby in the same clade T.
a. auratus and T. a. auratus haplotype aaC. We concluded that both of them belong to T. a. auratus subspecies.


Keywords: Javan Langur (Trachypithecus auratus), Javan Langur Center (JLC), Cytochrome-b

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