ANTIMALARIAL POTENTIAL STUDY OF CUCUMBER SKIN AND BASE POINT CHLOROFORM EXTRACT AS A SOLUTION TO ORGANIC WASTE OF SIDEWALK FOOD STALL TREATMENT IN YOGYAKARTA
Malaria is a major infectious disease in the world. The disease is caused by blood protozoan from the genus Plasmodium. The main problem in controling this disease is resistance parasite cases to drugs that have been used. Cucumber (Cucumis sativa L.) contain bioactive compounds suspected of terpenoids and saponins are believed to reduce the level of parasitemia. Waste of skin and cucumber base is very abundant, especially from stalls in the city of Yogyakarta. Estimated at the base and cucumber skin there are bioactive content that can be used as an alternative antimalarial drug. This research aims to study the potential of chloroform extract and the base of the fruit peel waste to the level of parasitemia of Plasmodium berghei in mice.
First step is collecting the waste of skin and cucumber base in some stalls in the city of Yogyakarta. Extraction by maceration method using chloroform solvent, the method further phytochemical studies by Thin Layer Chromatography (TLC). Antiplasmodium test with negative control treatment (DMSO 0.3%), positive control (chloroquine 3 mg / kg), the dose C.sativa extract 100; 200; 300; 400 and 500 mg / kg in male mice given strain Switzerland 3 months of age infected with Plasmodium berghei orally. T he results showed there were terpenoids and saponins compounds in the chloroform extracts of C.sativa base and skin. The most effective dose of the extract inhibition of parasitemia level P. berghei in mice treated P5 is the highest (500 mg / kg BB), which is still higher than the standard drug Chloroquine so that waste of skin and the base C.sativa potential as an alternative antimalarial drug.
Keywords: Waste of skin and the base of cucumber (Cucumis sativa L.) Antimalarial.
Anonim1. 2010. What is malaria?. http://www.malaria.org index.php?option=com. content &task=section&id=8&Itemid=32
Anonim2. 2008. Indonesia masih menjadi negara endemis tinggi malaria. http: //www.pdpersi.co.id.
Anonim3. 2005. Parasite control. Nature Reviews/Immunology. Nature Publishing Group.
Finney, D.J. 1971. Analysis Probit. 3th edition.Cambridge University Press. Britain.
Fujioka, H., and M. Aikawa. 2002. Structure and life cycle. http://www.online.karger.com/ ProdukteDB/Katalogteile/isbn3_8055/73/76/CI8Fujioka
Food Composition Table for Use in East Asia.1972. US Departement of Health, Education and Welfare, FDA UN,
Harborne, J.B. 1987. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Chapman & Hall Inc. London
Herbert, R.B. 1995. Biosintesis metabolit sekunder. Diterjemahkan oleh Bambang Srigandono. Semarang: IKIP Semarang Press.
Janse, C., and A. Waters. 2007. The Plasmodium berghei research model of malaria. www.lumc.nl/ 1040/research/malaria.model05.html.
Khomsan, A. 2009. Rahasia Sehat Dengan Makanan Berkhasiat. Penerbit Buku Kompas. Jakarta.
Levine, N.D. 1995. Protozoologi veteriner. Gadjah Mada University Press.Yogyakarta
Lohombo-Ekomba. 2004. Antibacterial, antifungal, antiplasmodial and cytotoxic activities of Albertisia villosa. Journal of ethnopharmacy. 93 (2004) 331-335.
Peter, Y. 1970. Technique for study of drug response in experimental malaria chemotherapy and drug resistance in malaria. New York: Academic Press.
Smyth, J.D. 1994. Introduction to Animal Parasitology. Third Edition. Cambridge University Press.Cambridge.