Centella asiatica sp. (pegagan) has neurotropic and neuroprotective properties which inhibit deterioration of memory because of chronic electrical stress. Studies about effective dose of Centtela asiatica still continued. This study was conducted to discover the influence of pegagan extract’s dose variation intake towards memory after chronic stress. This study uses that true experimental design with pretest-posttest control design. Fifteen male Sprague Dawley rats were divided into three groups randomly. Every groups are treated with chronic electrical stress for 4 weeks and aquades (KN), 300 (KN1) or 600 mg/kgBW/day (KP2) of Centella asiatica. The escape latency distance measure using Morris water maze was used to assess rats’ memory. Data were analyzed using the Kolmogorov-Smirnov, ANOVA and paired t-test. The average of escape latency distance before chronic electrical stress were 5,71±0,77m (KN); 4,29±0,36m (KP1); 3,81±0,47m (KP2) and after chronic electrical stress were 1,50±0,05m(KN); 1,55±0,04m(KP1); 1,82±0,11m(KP2). There were significant differences among groups after chronic electrical stress on day-1 (p=0,005) and day-3 (p=0,001). There were significant differences (p<0,05) for 3 days of escape latency test (KN), 5 days of escape latency test (KP1) and 2 days of escape latency test (KP2) from 6 days of memory measurement. In conclusion, dose variation of Centella asiatica did not affect to latency distance using Morris water maze. Ethanol extract of Centella asiatica with doses 300mg/kgBW/day could stimulate increasing memory formation after chronic electrical stress better.

Keywords: Centella asiatica - chronic electrical stress– memory– Morris water maze.

Alvin, V. Terry. Jr. 2009. Methods of Behavior Analysis in Neuroscience, 2nd edition : Chapter 13 Spatial Navigation (Water Mask) Tasks. Boca Raton (FL) : CRC Press.
Astari, N.A., D.C.R. Sari, S. Aswin, and S. Suharmi. 2012. The effect of administration pegagan etanolic extract on the number of hippocampal CA3 pyramidal neurons. Proceedings of the 23rd European Students’ Conference. 333; Germany: Cdayté.
Belcaro, G., F.X. Maquart, M. Soccianti, M. Dugall, M. Hosoi, M.R. Cesarone. 2011. TECA (Titrated Extract of Centella asiatica): new microcirculatory, biomolecular, and vascular application in preventive and clinical medicine. Panminerva Med 53 (3 Suppl 1): 105­18.
Bowman, R.E., K.D. Beck, and V.N. Luine. 2003. Chronic stress conditions effects on memory: sex differences in performance and monoaminergic activity. Hormone and Behavior . 43: 48-59.
Corwin, E.J. 2009. Buku Saku Patofisiologi. 3rd Ed. Jakarta: EGC.
Grossmann, M., and M.H. Skinner. 1996. A simple computer based system to analyze Morris water maze trials on-line. J.Neurosci. Method 70: 171-175.
Gupta, R., and S.J. Flora. 2006. Effect of Centella asiatica on arsenic induced oxidative stress conditions and metal distribution in rats. J Appl Toxicol 26(3):213-22.
Holmes, T.H., and R.H. Rahe. 1967. The Social Readjusment Scale. J. Psychosomatic Research. 11: 213-8.
Hooge, R., and P.P.D. Deyn. 2001. Application of the Morris water maze in the study of learning and memory. Brain Research Reviews. 36: 60-90.
Irawan, V., R. Susilowati, Y. Taihuttu, N. Wiyono, and T.W. Pangestiningsih. 2012. Protective Effect of Centella asiatica (L.) Urban Etanolic Extract on Hippocampal Call Proliferation of Adult Rat in Chronis Stress conditions Condition. Proceedings Pertemuan Ilmiah Nasional PAAI XIV. 60; Denpasar: Komisariat PAAI Denpasar.
Krzak, J.S., I.Z. Lupina, K. Czerny, M. Stepniewska, and A. Wrobel. Neuroprotective effect of ACTH (4-9) in degeneration of hippocampal nerve cells cause dexamethasone: morphological, immunohistochemical and ultrastructural studies. Acta Neurobiol Exp 2003. 63: 1-8.
Kumar, A., A. Prakash, and S. Dogra. 2011. Centella asiatica Attenuates D-Galactose-Induced Cognitive Impairment, Oxidative and Mitochondrial Dysfunction in Mice. J. Alzheimer’s Disease :[9p.].
Magarinos, A.M., J.M.G. Verdugo, and B.S. McEwen. 1997. Chronic stress conditions alters synaptic terminal structure in hippocampus. Neurobiology 94: 14002-8.
Maggio, N., and M. Segal. 2010. Corticosteron regulation of synaptic plasticity in the hippocampus. Scientific World. 10: 462-9.
McEwen, B.S., J.S. Flier, and B.S. Underhill. 1998. Protective and damaging effects of stress conditions mediators. N. Engl.J. Med. 338(3): 171-9.
Mook-Jung, I., J.E. Shin, S.H. Yun, K. Huh, J.Y. Koh, H.K. Park. 1999. Protective effect of asiaticoside derivates against beta amyloid neurotoxicity. J.Neuri.Res 58(3):417-25.
Morris, R.G.M., E.I. Moser, G. Riedel, S.J. Martin, J. Sandin, M. Day. 2003. Elements of Neurobiological Theory of the hippocampus: the role of activity-dependent synaptic plasticity in memory. Phil. Trans. R.Soc. Lond. 773-786.
Oktanindi, F. 2009. Pengaruh pemberian ekstrak etanol pegagan (Centella asiatica L.) terhadap memori spasial. Yogyakarta: Universitas Gadjah Mada. Skripsi.
Orhan, I.E. 2012. Centella asiatica (L.) Urban: From Traditional Medicine to Modern Medicine with Neuroprotective Potential. Eviandce-Based Complementary and Alternative Medicine. [8p.].
Rao, K.G.M., S.M. Rao, and S.G. Rao. 2007. Enhancement of Amydaloid Neural Dendritic Arboriderivateion by fresh Leaf Juice of Centella asiatica (Linn) during Growth Spurt Period in Rats. eCam 1-8.
Sari, D.C.R. 2011. The Relationship between Memory, CA1 Hippocampus and Neuroglia after Centella asiatica Leaf Extract’s Administration in Stress conditions-Induced Rat (Rattus novergicus). Proceedings of the 2nd International Joint Symposium. 12-9; Yogyakarta: Faculty of Med Univ Gadjah Mada.
Sari, D.C.R., S. Aswin, S. Suharmi, M. Romi, and U. Tranggono. 2012. The relationship between spatial memory and the thickness of hippocampal CA1 pyramidal layer in Stress conditions-induced rats (Rattus novergicus) after Centella asiatica leaf extract’s administration. Nutrition&Dietetics. 69 (Suppl 1 September 2012): 62.
Sasmita, P.K., T. Djuartina, and H. Uinarni. 2012. Effect of Extract of Gotu Kola (Centella asiatica) on Cogntive Function in Elderly. Proceedings Pertemuan Ilmiah Nasional Perhimpunan Ahli Anatomi Indonesia X1V. 60; Denpasar: Komiariat PAAI Denpasar.
Soumyanath, A., Z. Yong-Ping, E. Henson, Wadsworth, J. Bishop, and B.G. Gold. 2012. Centella asiatica Extract Improves Behavioral Deficits in a Mouse Model of Alzheimer’s Disease: Investigation of a PossibleMechanism of Action. Int. J. Alzheimer’s Disease [9p.].
Subathra, M., S. Shila, M.A. Devi, and C. Panneercellvam. 2005. Emerging role of Centella asaitca in imporeving age-related neurological antioxiandt status. Exp Gerontol 40(8­9): 707-15.
Tak, P.W., J.G. Howland, J.M. Robillard, Y. Ge, W. Yu, A.K. Titterness. 2007. Hippocampal long-term depression medates acte stress conditions induced spatial memory retrieval impairment. PNAS. 104(27):11471-6.