POTENCY OF BRASSICACEAE EXTRACTS AS AN APOPTOSIS ENCHANCER IN BLOOD CELLS MICE EXPOSED TO LEAD ACETATE
Abstract
Lead (Pb) is an environmental pollutant that is widely used in various industries. It has genotoxic effect that can trigger cancer formation (carcinogenic). The member of Brassicaceae family (white headed cabbage, pak choi, and Chinese cabbage) is known to have glucosinolates, isothiocyanates, phenethilisothiocyanates, and alyl isothiocyanates which are anticarcinogenic. The aim of this study was to examine the potential of Brassicaceae extracts to enhance apoptosis in mouse blood cell exposed to lead acetate. The research method was descriptive with five treatments and four replications. Mice were pretreated with white headed cabbage, pak choi, and Chinese cabbage extracts by cumulative dose 156 mg/kg bw for 13 days prior to exposure of lead acetate by cumulative dose 150 mg/kg bw. All treatment was given orally. Blood samples were taken on the thirteenth day from the tail venous vessel. Furthermore, the DNA isolation and electrophoresis were done to examine DNA fragmentation. The result showed that the white headed cabbage extract enhanced the formation of a 200 bp DNA ladder pattern that is characteristic of apoptosis in the mice blood cells due to lead acetate exposures.
Keywords: apoptosis, lead acetate, white headed cabbage, pak choi, Chinese cabbage
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