Brown planthopper is one of the most destructive insect pest of rice in Indonesia and other Asian countries. Pyramiding some brown planthopper resistance genes is a valuable approach to create more durable resistance against the pest. The objective of this study was to identify polymorphisms of Brown Planthopper Resistance genes (Bph) on 20 genotypes of rice, and to obtain genetic relationship among genotypes tested. The experiment was conducted from June to September 2012 at Green House and Laboratory of Plant Analysis and Biotechnology, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor. Twenty genotypes were analyzed, and two of them were used as check varieties. Simple Sequence Repeat (SSR) markers were applied to detect Bph3, Bph4, Qbph3, and Qbph4 genes. Polymorphic levels were analyzed by calculating PIC (Polymorphic Information Content). The grouping of rice genotypes were done based on principal components analysis (PCA) of SSR data, and the genetic relationship based on the presence of Bph genes was estimated using UPGMA (Unweighted Pair Group With Arithmetic mean). Results showed that RM313, RM8072, RM8213, RM5953, RM586, and RM589 markers were polymorphic. Rice genotypes PTB 33, Diah Suci, Cibogo, Cisantana, Digul, Ciherang, Inpari 13, Inpari 10, and Memberamo had Bph3, Qbph3, Bph4, and Qbph4. Meanwhile Bph3, Qbph3, and Bph4 were supposed to be belonged by IR 64, Aek Sibundong, Batang Gadis, IR 66, and Mekongga. Kalimas and Tukat Penatu had Bph3, Qbph3, dan Qbph4. IR 74 had Bph3 and Qbph3, and Fatmawati had Bph3 and Bph4. UPGMA clustering resulted in two main clusters, in which the first cluster consisted of 2 subclusters. PTB-33 was closely related with Memberamo, Tukat Penatu, Digul, Diah Suci, and Kalimas. The SSR markers used in this study were proven to be valuable in molecular detection of Bph genes and in estimating genetic relationsips of rice genotypes. PTB-33 was a good donor of resistance genes, as well as Memberamo, Tukat Penatu, Digul, Diah Suci, and Kalimas which were identified as promising donors in rice breeding resistance to brown planthopper.

Keywords : Bph gene, Brown Planthopper, Genetic relationship, SSR markers.

Anderson, J.A., G.A. Churchill, J.E. Autrique, S.D. Tanksley, and M.E. Sorrells. 1993. Optimizing parental selection for genetic linkage maps. Genome 36: 181-186.
Baehaki. 2007. Population development of brown planthoppers biotype 4 (in Bahasa Indonesia). Jakarta: Gramedia.
Bardakci, F. 2001. Random amplified polymorphic DNA (RAPD) markers. Turk. J. Biol. 25:185-196.
Botstein, D., R.L. White, M. Skolnick, and R.W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314– 331.
Caetano-Anollés, G. 2004. DNA amplification fingerprinting. a forum for DNA marker methodologies: University of Illinois at Urbana- Champaign.
Chen, H.A. 2000. PCR. Chen’s own protocols: Chen’s protocol list: PCR. http://users.
Chen, J.W., L. Wang, X.F. Pang, and H.Q. Pan. 2006. Genetic analysis and fine mapping of a rice brown planthopper (Nilaparvata lugens Stål) resistance gene bph19(t). Mol. Genet. Genomics 275: 321-329.
Doyle, J.J., and E.E. Dickson. 1987. Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36:715-722.
Foulet, F., N. Nicolas, O. Eloy, F. Botterel, J.C. Gantier, J.M. Costa, and S. Bretagne. 2005. Microsatellite marker analysis as a typing system for candidate glabrata. Journal of Clinical Microbiology 43(9) : 4574-4579.
Gramene. 2008. Primer. species=Oryza_sativa&q=primer. Accessed 2 January 2012.
Jairin, J., K. Phengrat, S. Teangdeerith, A. Vanavichit, and T. Toojinda. 2007a. Mapping of a broadspectrum brown planthopper resistance gene, Bph3, on rice chromosome 6. Mol. Breed., 2007a, 19, 35-44.
Jairin, J., S. Teangdeerith, P. Leelagud, K. Phengrat, A. Vanavichit, and T. Toojinda. 2007b. Physical mapping of Bph3, a brown planthopper resistance locus in rice. Mj. Int. J. Sci. Tech. 2007b, 01(2), 166-177
Kisimoto, R. 1981. Development, behavior, population dynamics and control of the brown planthopper, Nilapavarta lugens Stal. Rev. Plt. Protect. Res. 14:26-58.
Liu, B.H. 1998. Statistical genomics, linkage mapping, and QTL analysis. CRC Press. New York. p.45-83
Mochida, O., T. Suryana, Hendarsih, and A. Wahyu. 1978. In The Brown Planthopper. Proc. Symp. Brown Planthopper. The 3rd Inter – Congress of the Pacif. Sci. Ass., Bali, Indonesia, 22-23 July 1977. p. 1-39.
Murata, K. 1998. Molecular linkage analysis of brown planthopper resistance genes in rice. The Graduate School of Science and Technology, Kobe University.
Nugaliyadde, L., D.M.N. Dissanayake, and J. Mitrasena. 2000. Advances of pest and disease management of rice in Sri Lanka. Department of Agriculture, Peradeniya. Sri Lanka.
Rohlf, F.J. 1998. Numerical taxonomy and analysis system (NTSYSpc) version 2.0. New York: Department of Ecology and Evolution Sate University of New York.
Sharma, P.N., A. Tori, S. Takumi, N. Mori, and C. Nakamura. 2003. Marker-assisted pyramiding of brown planthopper (Nilapavarta lugens Stal) resistance gene Bph1 and Bph2 on rice chromosome 12. Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, and Division of Life Science, Graduate School of Science and Technology, Kobe University, Kobe, Japan.
Sun, L., C. Su, C. Wang, H. Zhai, and J. Wan. 2005. Mapping of a major resistance gene to the brown planthopper in the rice cultivar Rathu Heenati. Breed. Sci. 55: 391-396.
Suprihatno, B., A.A. Daradjat, Satoto, S.E. Baehaki, Suprihanto, A. Setyono, S.D. Indrasari, I.P. Wardana, and H. Sembiring. 2010. Description of Rice Varieties (In Bahasa Indonesia: Deskripsi Varietas Padi). BB Penelitian Tanaman Padi. Badan Penelitian dan Pengembangan Pertanian. Departemen Pertanian.
Zhang, Y., X.F. Wang, Z.K. Li, G.Y. Zhang, and Z.Y. Ma. 2011. Assessing genetic diversity of cotton cultivars using genomic and newly developed expressed sequence tag-derived microsatellite markers. Hebei Agricultural University, Baoding, China. Genetics and Molecular research 10 (3): 1462-1470.