Breast cancer is one of cancer with high mortality. This cancer not only attacks women, but also men. Indonesia has many plants which potential as anticancer, such as orchids. Spathoglottis plicata is one of the orchid species that abundant in Indonesia and has a lot of antioxidant compounds which is guessed have anticancer properties. The objectives of this study were to study the cytotoxic activity and IC50 value of aquadest, ethanolic, and chloroform extracts of S. plicata’s pseudobulbs, leaves, and whole plants on T47D cells (breast cancer cells line) as well as cytotoxic activity of the specific fraction of the most toxic crude extract. S. plicata used in this study was obtained from Bungarinte nursery. Extractions were done by maceration method using aquadest, ethanol, and chloroform as the solvent. Cytotoxic test on T47D cells were done by MTT assay. The cytotoxic data were analyzed using one-way ANOVA followed by Tukey’s HSD test. The IC50 of each extracts were calculate by probit analysis. The lowest IC50 value among all extracts was fractionated and isolated by preparative TLC. The cytotoxic activity and IC50 of this fractions were analyzed. The results showed that only 2 from 9 crude extracts that able to calculate its IC50 because those two extracts have concentration dependent pattern of inhibition concentration. Chloroform extract have the lowest IC50 value (369,837 μg/mL). Then, this extract fractionated by eluen n-hexane : ethyl acetate 4:1. Four fractions were collected. The lowest IC50 value is fraction IV (144,41 μg/mL). Based on the results it could be concluded that S. plicata leaves have moderate potency to develop as anticancet agents, especially on breast cancer.

Keywords: S. plicata, T47D cells, cytotoxic, MTT assay, preparative TLC. 

Agarwal, N., C. Majee, and G.S. Chakraborthy. 2012. Natural herbs as anticancer drugs. International Journal of Pharm Tech Research, Vol. 4(3): 1142-1153.
Da’i, M., U.A. Jeniel, A.M. Supardjan, M. Kawaichi, and E. Meiyanto. 2007. T47D cells arrested at G2M and hyperploidy formation induced by a curcumin’s analogue PGV-1. Indonesian Journal of Biotechnology, Vol 12(2) : 1007.
Gutiérrez, R.M.P. 2010. Orchids: A review of uses in traditional medicine, its phytochemistry and pharmacology. Journal of Medicinal Plants Research, Vol. 4(8): 592-638
Histopaedianto, I., L. Choridah, and T. Aryandono. 2008. Validity of BI-RADS System Mammography to detect breast cancer at Dr. Sardjito Hospital Yogyakarta. Berkala Ilmu Kedokteran, Vol. 40 (1) 20-25.
Holle, M. J. M., F. R. Arfriana, and W.A.S. Tunjung. 2013. Detection of alkaloid, flavonoid, and terpenoid compounds of ethanolic and chloroform of pseudobulbs and leaves extracts on Dendrobium mutabile (Blume) Lindl. and Spathoglottis plicata Blume, Proceeding of The 10th Hokkaido Indonesian Student Association Scientific Meeting : 1-5
Kanadaswami, C., L. Lee, P.H. Lee, J. Hwang, F. Ke, Y. Huang, and M. Lee. 2005. The antitumor activities of flavonoids. in vivo, Vol. 19: 895-910
Ren, W., Z. Qiao, H. Wang, L. Zhu, and L. Zhang. 2003. Flavonoids: promising anticancer agents. Medicinal Research Reviews, Vol. 23 (4): 521-528
Masuda, Y., J. Ueda, M. Tamura, H. Sakagami, M. Tomomura. A. Tomomura and Y. Shirataki. 2011. Diverse biological activity on Odontoglossum Harvengtense ‘Tutu’ bulb extracts. In Vivo, Vol. 25: 381-386
Nuriliani, A., N. Wijayanti, A. Kusmiyati, Y. A. Purwestri, and R. Topriyani. 2013. Growth inhibition, apoptosis induction, and antiangiogenesis activity of ethanolic extract of Dendrobium crumentum Swartz. Proceeding of The First Annual International Scholar Conference in Taiwan, ISSN:2337-442X : 587-595.
Rahmawati, Z.N, 2009. Evaluasi penggunaan antiemetic dalam penatalaksanaan mual muntah karena kemoterapi pada pasien kanker payudara di RSUD Dr Moewardi Surakarta tahun 2008. Skripsi. Universitas Negeri Sebelas Maret Surakarta.
Ratnawati, H., W. Widowati, D.K. Jasaputra, and S. Soeng, 2008. Cytotoxic activity of buah merah fractions (Pandanus conoideus Lam.) towards cervical cancer cell in HeLa cells culture. Proceeding ot The International Seminar on Chemistry, P:317-320.
Sampath, M., and Vasanthi M. 2013. Isolation, structural elucidation of flavonoids from Polyathia longifolia (Sonn.) thawaites and evaluation of antibacterial, antioxidants, and anticancer potential. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 5: 336-341.
Sandra, E. 2004. Kultur Jaringan Anggrek Skala Rumah Tangga. AgroMedia Pustaka., Jakarta. P: 1
Sathiya, M., and K. Muthuchelian. 2010. Antitumor potential of total alkaloid extract from Tabebuia rosea (Bertol.) DC. leaves on MOLT-4 cells in vitro. Nature and Science, Vol. 8(9): 7
Sheehan, T., and M. Sheehan. 1994. An illustrated survey of orchid. Cambridge University Press. Cambridge.
Shriram, V., V. Kumar, P.B.K. Kishor, S.B. Suryawanshi, A.K. Upadhyay, and M.K. Bhat. 2010. Cytotoxic activity of 9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol from Eulophia nuda against human cancer cells. Journal of Ethnopharmacology, Vol. 128 : 251–253
Thoppil, R.J., and A. Bishayee. 2011. Terpenoids as potential chemopreventive and therapeutic agents in liver cancer. World J Hepatol, Vol. 3(9) : 228-232.
Wu, P., Y. Hsu, and C. Jao. 2006. Indole alkaloids from Cehpalanceropsis gracilis. American Chemical Society and American Society of Pharmacognosy.
Xia, G., R. Matsidik, M. Ablise, S. Lei, and A. Abudula. 2013. Anti-cancer activity of flavonoids from Xinjiang Glycyrrhiza inflata Licorice on proliferation, cytotoxicity and apoptosis in cervical carcinoma cells. Journal of Medicinal Plants Research, Vol. 7(5): 173-178.