Aloe veragelhas a large vitamins which has become potential candidate to supressed vascular damaged oforganwhich potentially lead to complications such as diabetic nephropathy. This process through down regulation of PKC-β level as a cascade activator of gene transcription such as contraction and permeability of cell. The research goal was to determinethe effect of Aloe vera gel on PKC-β level in the kidneys of diabetes mellitus (DM) wistar rats. Twenty four rats divided into eight treatments (negative controls, positive controls, Non DM with A.vera gel dosage 30, 60, and 120 mg/day, DM with A.vera gel dosage 30, 60 and 120 mg/day) with three replications each. All of the data was analyzed using one way Anova with Statistical Product and Service Solution 16 software. The research result showed that the A. vera gelhas significant effectto decrease PKC-β level significantly (p < 0,000) in diabetes mellitus (DM) wistar rat kidney. In conclusion A.vera gel supressed diabetic nephropathy process via PKC-β and ROS activity in optimum dosage 30 mg/ day.

Keywords: Aloe vera Gel, PKC-β level, Diabetes mellitus. 

American Association of Diabetes Educators. 2003. AADE core curriculum: Diabetes and Complications 5th ed. Chicago, Illinois; American Association of Diabetes Educators. pg:158-159.
Corwin, E. J. 2009. Departemen Kesehatan Republik Indonesia. 2011. Tahun 2030 Prevalensi Diabetes mellitus di Indonesia Mencapai 21,3 Juta Orang. index.php/berita/press-release/414-tahun-2030-prevalensi-diabetes-melitus-di- indonesia-mencapai-213-juta-orang.html, accessed 11 September 2011.
El-Missiry, M.A., and A.I. Othman. 2004. AmerL-Arginine Ameliorates Oxidative Stress in Alloxan-induced Experimental Diabetes Mellitus. J. Appl. Toxicol. 24, 93–97.
Geraldes, P., and G.L. King. 2011. Activation of Protein Kinase C Isoform and Its Impact on Diabetic Complication. Circ. Res. 106; 1319-1331.
Joseph, B., and Raj. 2010. Pahrmacognostic and Phytochemical Properties of Aloe vera Linn. Malankara Catholic College.
Joy, S.V., A.C. Scates, S. Bearelly, M. Dar, C.A. Taulien, J.A. Goebel, and M.J. Cooney. 2005. Ruboxistaurin, a protein kinase C beta inhibitor, as an emerging treatment for diabetes microvascular complications.Ann Pharmacother. Oct;39(10):1693-9.
Koya, D., K. Hayashi, M. Kitada, A. Kashiwagi, R. Kikkawa, and M. Haneda. 2003. Effect of Antioxidants in Diabetes-Induced Oxidative Stress in the Glomeruli of Diabetic Rats. J Am.SocNephrol 14: S250-S253.
Meier, M., J. Menne, J-K. Park, and H. Haller. 2007. Nailing down PKC isoform specificity in diabetic nephropathy- two’s company, three’s a crowd. Nephrol Dial Transplant. 22: 2421-2425.
Ohshiro, Y., Y. Lee., and G.L. King. 2005. Mechanism of Diabetic Nephropathy : Role of Protein Kinase C Activation. Advanced Studies in Medicine. Vol. 5 1 (A).
Ramamoorthy, L., M.C. Kemp, and I.R. Tizard. 1996. Acemannan, a beta-(1,4)-acetylated mannan, induces nitric oxide production in macrophage cell line RAW 264.7. Mol Pharmacol. 50(4):878-84.
Ramamoorthy, L., and I.R. Tizard. 1998. Induction of apoptosis in a macrophage cell line RAW 264.7 by acemannan, a beta-(1,4)-acetylated mannan. MolPharmacol. 53(3):415- 21.
Ricky, D. 2007. Pengaruh Ekstrak Etanol Daun Kemangi (Ocimum basilicum) terhadap Kadar Malondialdehid dan Glukosa Darah Tikus Jenis Wistar yang Diinduksi Aloksan Monohidrat (online)., accessed 24January 2011.
Roy, K., F. Harris, S.R. Dennison, D.A. Phoenix, and J. Singh. 2005. Effect of Streptozocin- induced type 1 diabetes mellitus on protein and ion concentrations in ocular tissues of the rat. Int J Diabetes & Metabolism. 13: 154-158.
Sonkodi, S., and A. Mogyorosi. 2003. Treatment of diabetic nephropathy with angiotensin II blockers. Nephrol Dial Transplant. 18 [Suppl 5]: v21–v23.
Steiner, D., R.S. Norberto, C. Gonzalez, and J.G. Wood. 2002. Interaction between reactive oxygen species and nitric oxide in the microvascular response to systemic hypoxia. J ApplPhysiol 93: 1411–1418.
Sunaryanto, A. 2010. Penatalaksanaan Penderita dengan Diabetik Nefropathy. Denpasar: FK UNUD.
Su, T.T. 2002. PKC beta controls I kappa B kinase lipid raft recruitment and activation in responsetoBCRsignaling.Nature.Immun.3: 780-786.
Whiteside, C.I., and J.A. Dlugosz. 2002. Mesangial Cell Protein Kinase C Isoenzyme Activation in The Diabetic Milieu. Am J Physiol Renal Physiol 282: F975-F980.