Measurement of Alkaloids Achyranthes Aspera Linn Level Using Thin Layer Chromatography Method and High-Performance Liquid Chromatography
Abstract
One of the plants with the potential to be developed into a raw material for anti-breast cancer drugs is jarong (Achyranthes aspera linn). Allegedly alkaloid of Achryranthes aspera linn leaves showed anticancer activity in vitro in mice myeloma cells is extremely potent. In vivo study showed that the alkaloid Achyranthes aspera linn causes apoptosis and healing in breast cancer cells in mice induced by benzopyrene. Alkaloids of Achyranthes aspera linn leaves as anticancer been listed on Incestual Property Rights (IPR) since October 12, 2012, with number P00201299839. This study aims to prove the existence of alkaloids in the leaves of Achyranthes aspera linn using thin layer chromatography (TLC), determine the level of alkaloids in the leaves alkaloid fraction of Achyranthes aspera using High-Performance Liquid Chromatography (HPLC) and alkaloid fraction dose as an anticancer and dosing for the study. Alkaloid qualitative measurement results with thin layer chromatography showed alkaloid in orange. Alkaloid levels using HPLC has a retention time of 3.482 minutes, a wavelength of 254 nm, there are 13 kinds of chemical substances with the major components amounted to 52.36% alkaloids, the other component is the green substance with a peak area of 13.0624%, while other chemical substances in a very small peak area of between 0.428% until 8.3598%. Alkaloid fraction dose of Achyranthes aspera used in the study as anticancer is controlled the dose of 0 mg/kg, 1 treatment dose of 60 mg/kg, 2 treatment dose of 75 mg/kg and 3 treatment dose of 90 mg/kg.
Keywords: Alkaloids, Achyranthes aspera linn, TLC, HPLC.
References
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