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Mahardika, M. M., Sudjarwo, S. A., & Koesdarto, S. (2017). Anthelmintic Activity of Ocimum sanctum Linn. Leaves Ethanol Extract Against Fasciola gigantica in vitro. KnE Life Sciences, 3(6), 266-277. https://doi.org/10.18502/kls.v3i6.1135

https://doi.org/10.18502/kls.v3i6.1135

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authors

  • Mesia Margi Mahardika

    Mesia Margi Mahardika

    Student Faculty of Veterinary Medicine, Universitas Airlangga
  • Sri Agus Sudjarwo

    Sri Agus Sudjarwo

    Departement of Basic Veterinary Medicine Faculty of Veterinary Medicine, Universitas Airlangga
  • Setiawan Koesdarto

    Setiawan Koesdarto

    Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga

Published Date

  • Dec 3, 2017

Anthelmintic Activity of Ocimum sanctum Linn. Leaves Ethanol Extract Against Fasciola gigantica in vitro

KnE Life Sciences / The Veterinary Medicine International Conference (VMIC) / Pages 266-277

Abstract

The aims of this research are to know the concentration, exposure time and interaction between concentration and exposure time of Ocimum sanctum Linn. leaves ethanol extract which cause the most mortality toward Fasciola gigantica. Also to know its value of LC50 and LC90. The research was completely randomized design. There were five treatments. Each treatment was done in four replications and used 10 Fasciola gigantica. The observation and recording of dead Fasciola gigantica were done at 0, 2, 4, 6, 8 and 10 hours. Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (50ºC). The obtained data was analyzed using ANOVA Factorial and continued with Duncan Multiple Range Test. The result was 10% concentration and exposure time for 10 hours caused the most mortality toward Fasciola gigantica. However, the interaction between concentration and exposure time resulted that 5% concentration for 8 hours already caused the most mortality of Fasciola gigantica. Probit analysis was used to calculate the LC50 and LC90. The results were LC50 of Ocimum sanctum Linn. leaves ethanol extract was 7.9% at 4 hours, 3.7% at 6 hours, 1.8% at 8 hours and 0.8% at 10 hours and the LC90 was 8.4% at 10 hours.

 

Key words: Ocimum sanctum Linn. leaves, Fasciola gigantica, ethanol extract, in vitro.

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