Download fulltext HTML
Suprayogy, T. W., Susilowati, S., & Hernawati, T. (2017). Adding of L-Arginin Amino Acidin Skim Milk Diluent to Maintain Quality of Buck Sperm in Cold Temperature. KnE Life Sciences, 3(6), 189-196. https://doi.org/10.18502/kls.v3i6.1127

https://doi.org/10.18502/kls.v3i6.1127

statistics

  • 427 Abstract Views
  • 234 PDF Views
  • 0 HTML Views

authors

  • Tri Wahyu Suprayogy

    Tri Wahyu Suprayogy

    Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya
  • Suherni Susilowati

    Suherni Susilowati

    Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya
  • Tatik Hernawati

    Tatik Hernawati

    Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya

Published Date

  • Dec 3, 2017

Adding of L-Arginin Amino Acidin Skim Milk Diluent to Maintain Quality of Buck Sperm in Cold Temperature

KnE Life Sciences / The Veterinary Medicine International Conference (VMIC) / Pages 189-196

Abstract

Nowdays, the storage of buck semen in cold temperature have not satisfied yet  because in  buck’s seminal plasma contains phospholipase enzyme which can coagulated egg yolk in diluents.The specific aim of this study was to investigate the benefits of  L-Arginin amino acid in skim diluents to quality  buck’s spermatozoa on cold temperature. This researchutilized four treatment groups, namely Controlled group (P0): skim milk diluent without L-Arginin + buck’s semen; P1: skim milk diluents + L-Arginin 0,002M/ml + buck” semen; P2: skim milk diluents + L-Arginin 0,004 M/ml + buck’s semen and P3: skim milk diluents + L-Arginin 0,006 M/ml + buck’s semen. Then the samples stored in cold temperature (5oC). The result showed that sperm motility, viability and membrane integrity were significantly different (p<0,05) among the treatments.The conclusion of this study is adding of L-Arginin Amino Acid in skim milk diluents maintain motility, viability and membrane integrity  buck’s sperm.

 

Keywords: L-Arginin, buck, cold temperature, motility, viability and membrane integrity

References
[1]. Direktorat Jenderal Produksi Peternakan, 2007. Petunjuk Teknis Produksi dan Distribusi Semen Beku. Departemen Pertanian. Jakarta
[2]. Lessard, C.,S. Parent, P. LEclerc, J.L. bailey and R. Sullivan, 2000. Cryopreservation Alters the Levels of the Bull Sperm Surface Protein P25b. Journal Andrology.21:700-707.
[3]. Supriatna, I dan Pasaribu,FH, 1992. In Vitro Fertilization. Transfer Embrio dan Pembekuan Embrio. Bogor. PAU.IPB.
[4]. Kasai, M. 1996. Simple and Efficient Methods for Vitrification of Mammalian Embryos. Animal Reproduction Sciences. 42(1): 67-75.
[5]. Alvarez, J.G and BT Storey, 1995. Differential Incorporation of Fatty Acid Into and Peroxidative Loss of Fatty Acid from Phospholipid of Human Spermatozoa. Mol. Reprod.Dev. 42: 334-345.
[6]. Garner and Hafez, E.S.E, 2000. Reproduction in Farm Animal. 7th Edition. Philadelphia. Baltimore. New York London.
[7]. Susilowati,S. Potensi Biologis Insulin Like Growth Factor-I Complex Plasma Seminalis Kambing Hasil Sentrifugasi. Disertasi. Pasca Sarjana Fakultas Kedokteran Universitas Airlangga.
[8]. Srivastava,S., Prashant D., Evans C., Girjesh G. 2006. Mechanism of Action of L-Arginin on The Vitality of Spermatozoa is Primarily Through Increased Byosynthesis of Nitric Oxide. Biology of Reproduction 74: 954-958.
[9]. Al-Ebady,AS., SO. Hussain, KI.,Al-Badry and FF. Ibrahim.2012. Effect of Adding L-Arginin on Some Parameters of Bull Sperms After Freezing in Liquid Nitrogen (-196oC). Al-Qadisiya Journal of Vet Med Sci. 11(2):15-161.
[10]. Winarsi, H. 2007. Antioksidan Alami dan Radikal Bebas. Kanisius. Yogyakarta.
[11]. Yanagimachi, 1994. Mammalian Fertilization. In : The Physiology of Reproduction. E. Knobil, J. Neil, L.L. GS. Greenwald, C.L. Market, DW. Plaff. Raven Pers LTD. New York.