Download fulltext HTML
Restiadi, T. I., Mustofa, I., Utama, S., & Mulyati, S. (2017). The Effect of Insulin-Like Growth Factor-I of Pregnant Crossbred Mare Serum and Insulin-Like Growth Factor-I Recombinant Mouse on Estrous Cycles and Litter Sizes of Mice (Mus musculus). KnE Life Sciences, 3(6), 175-188. https://doi.org/10.18502/kls.v3i6.1126

https://doi.org/10.18502/kls.v3i6.1126

statistics

  • 398 Abstract Views
  • 341 PDF Views
  • 0 HTML Views

authors

  • Tjuk Imam Restiadi

    Tjuk Imam Restiadi

    Department of Veterinary Reproduction, Faculty of Veterinary Medicine Universitas Airlangga – Surabaya 60115
  • Imam Mustofa

    Imam Mustofa

    Department of Veterinary Reproduction, Faculty of Veterinary Medicine Universitas Airlangga – Surabaya 60115
  • Suzanita Utama

    Suzanita Utama

    Department of Veterinary Reproduction, Faculty of Veterinary Medicine Universitas Airlangga – Surabaya 60115
  • Sri Mulyati

    Sri Mulyati

    Department of Veterinary Reproduction, Faculty of Veterinary Medicine Universitas Airlangga – Surabaya 60115

Published Date

  • Dec 3, 2017

The Effect of Insulin-Like Growth Factor-I of Pregnant Crossbred Mare Serum and Insulin-Like Growth Factor-I Recombinant Mouse on Estrous Cycles and Litter Sizes of Mice (Mus musculus)

KnE Life Sciences / The Veterinary Medicine International Conference (VMIC) / Pages 175-188

Abstract

The research was conducted to determine the effect of insulin-like growth factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) on estrous cycle and litter size in mice (Mus musculus). The research was arranged by Completely Randomized Design (CRD) with seven treatments and five replications. The treatments were control (CO)= physiological NaCl, treatment (T) 1 = 10 ng/ml of IGF-I pregnant mares crossbred serum (PMS), T2 = 20 ng/ml of IGF-I PMS, T3 = 40 ng/ml of IGF-I PMS, T4 = 10 ng/ml of IGF-I recombinant mouse, T5 = 20 ng/ml of IGF-I recombinant mouse, and T6 = 40 ng/ml of IGF-I recombinant mouse. Serie I the subject of this research were 35 female mice. Observed variables included estrous cycle phases that proestrus, estrus, metestrus, and diestrus phase. Serie II for the objects of this research were 35 female mice and 21 male mice in the same treatment on litter size in mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS did not significantly affect (p>0.05) on proestrus, metestrus, and diestrus phase. The addition of IGF-I PMS 40 ng/ml was extended (p<0.05) the estrus phase. It showed that there was not significantly difference (p>0.05) between the effect of IGF-I PMS and IGF-I recombinant mouse with the mice estrous cycles. The result of IGF-I derived from PMS and IGF-I recombinant mouse on litter size in mice did not significantly difference (p>0.05). Test results shows significant differences between control and 40 ng/ml of IGF-I PMS.

 

Keywords: IGF-I, IGF-I pregnant crossbred mare serum, estrous cycle, litter sizes, Mus musculus

References
[1] G. Oberlender, L.D.S. Murgas, M.G. Zangeronimo, A.C. da Silva, T.A. Menezes, T.P. Pontelo, L.A. Vieira, Role of Insulin-Like Growth Factor-I and Follicular Fluid from Ovarian Follicles with Different Diameters on Porcine Oocyte Maturation and Fertilization In Vitro. Theriogenology 80 (2013) 319-327.
[2] J.A. Neira, D. Tainturier, M.A. Pen., J. Martal, Effect of The Association of IGF-I, IGF-II, bFGF, TGF-b1, GM-CSF, and LIF on The Development of Bovine Embryos Produced InVitro. Theriogenology73 (2010) 595-604.
[3] S. Singhal, S. Prasad, B. Singh, B., J.K. Prasad, and H.P. Gupta, Effect of Including Growth Factors and Antioxidants in Maturation Medium used for In Vitro Culture of Buffalo Oocytes Recovered In Vivo. AnimReprod Sci. 113 (2009) 44-50.
[4] D.M. Magalhães-Padilhaa, A.B.G. Duarte, V.R. Araújo, M.V.A. Saraiva, A.P. Almeida, G.Q. Rodrigues, M.H.T. Matos, C.C. Campello, J.R. Silva, and M.O. Gastal, Steady-State Level of Insulin-Like Growth Factor-I (IGF-I) Receptor mRNA and The Effect of IGF-I on The In Vitro Culture of Caprine Preantral Follicles. Theriogenology 77 (2012) 206–213.
[5] W.M.M. Nalley, R. Handarini, M. Rizal, R.I. Arifiantini, T.L. Yusuf and B. Purwantara, Determination ofThe Estrous Cycle Based on Vaginal Cytology and Hormone Profile in Timor Hind. Jurnal Veteriner 12(2) 2011, 98-106.
[6] A. Inoue, T. Sakae, and T. Sumio, Insulin-Like Growth Factor-I Stimulated DNA Replication in Mouse Endometrial Stroma Cells. J. Reprod and Development 51 (2005) 3.
[7] C. Sumantri, M. Imron, Sugyono, E. Andreas, R. Misrianti, and A.B.L. Ishak, Growth Hormone Gene Family (GH, GHR, GHRH and Pit-1) Polymorphisms and Its Association with Superovulation Response, Ovulation Rate, Fertilization Rate and Embryo Quality in Embryo Transfer Station (BET) of Cipelang. JITV16(2) 2011, 126-139.
[8] M.A. Velazquez, M. Newman, M.F. Christie, P.J. Cripps, M.A. Crowe, R.F. Smith, and H. Dobson, The Usefulness of A Single Measurement of Insulin-Like Growth Factor-1 as A Predictor of Embryo Yield and Pregnancy Rates in ABovine MOET Program. Theriogenology 64 (2005) 1977-1994.
[9] R.A. Cushman, V.S. Hedgpeth, S.E. Echternjkamp, and J.H. Britt, Evaluation of Numbers of Microscopic and Macroscopic Follicles in Cattle Selected for Twinning. J.Anim.Sci. 78 (2000) 1564-1567.
[10] F.D. Jousan, and P.J. Hansen, Insulin-Like Growth Factor-I Promotes Resistance of Bovine Preimplantat Ion Embryos to Heat Shock Through Actions Independent of Its Anti-Apoptotic Actions Requiring PI3K Signaling. Mol.Reprod.Dev. 74 (2007) 189-196.
[11] J. Block, C. Wrenzycki, H. Niemann, D. Herrmann, and P.J. Hansen, Effects of Insulin-Like Growth Factor-1 on Cellular and Molecular Characteristics of Bovine Blastocyst Produced In Vitro. Mol.Reprod. Dev. 75 (2008) 895-903.
[12] G.A. Hendarti, Pengaruh Pemberian Kompleks Insulin-Like Growth Factor-I dan Insulin-Like Growth Factor Binding Protein-3 terhadap Perbaikan Reproduksi Mencit Betina (Mus musculus) [Tesis]. Program Pasca Sarjana. Universitas Airlangga, 2006.
[13] M.M. Rochler, Insulin-Like Growth Factor Binding Protein, Vitamins and Hormones 47 (1993) 111-114.
[14] T.R. Davidson, C.S. Chamberlain, T.S. Bridges, and L.J. Spicer, Effect of Follicle Size on In Vitro Production of Steroids and Insulin-Like Growth Factor (IGF)-I, IGF-II, and The IGF-Binding Proteins by Equine Ovarian Granulosa Cells. Biology Reproduction 66 (2002) 1640-1648.
[15] J.D. Wilson, D.W. Foster, M.K. Hendry, and P.R. Larsen, Endocrine Disease. William Text Book of Endocrinology 9th ed. W.B. Founder Saunders Company, 1998. 1437-1452.
[16] T.I. Restiadi, Potensi Biologis Bahan Bioaktif Insulin-Like Growth Factor-I Asal Serum Kuda Crossbred G3-G4 Bunting pada Fertilisasi In Vitro pada Sapi (Disertasi). Universitas Airlangga, 2013.
[17] Y. Wang, S. Nishida., H.Z. Elalieh., R.K. Long., B.P. Halloran., and D.D. Bikle, Role of IGF-I Signaling in Regulating Osteoclastogenesis. J. Bone and Mineral Research. 21 (2006) 123-128.
[18] G.N. Arunakumari, N. Shanmugasundaram, and V.H. Rao, Development of Morulae from The Oocytes of Cultured Sheep Preantral Follicles. Theriogenology 74 (2010) 884–894.
[19] Rintafiani, Siklus Estrus pada Mencit (Mus musculus) [Skripsi]. Program Pendidikan Biologi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Teknologi Sepuluh Nopember. Surabaya, 2014.
[20] G.C. Agustina, Pengaruh Pemberian Ekstrak Daging Buah Pare Hijau (Momordica chaantia L.) terhadap Siklus Birahi Mencit (Mus musculus) yang Disuperovulasi dengan PMSG dan hCG [Skripsi]. Fakultas Kedokteran Hewan. Universitas Airlangga. Surabaya, 2013.
[21] R.G.D. Steel, and J.H. Torrie, Principles and Procedures of Statistics, McGraw. Hill Co., New York, 2013, 137-269.
[22] E.S.E. Hafez, Reproduction in Farm Animal. 7th ed. Lippincott Williams & Wilkins, 2000, 48-52, 59-62, 68-80, 90-94, 196-200.
[23] L.A. Sutasurya, A.J. Sitasiwi, dan K. Ratnawati, Penentuan Kandungan Estradiol (E2) dan Luteinizing Hormone (LH) pada Petaurus breviceps papuanus (Marsupialia) selama Satu Siklus Estrus. Jurnal Sains dan Teknologi Nuklir Indonesia. 11 (2001) 25-35.
[24] M.H. Johnson, and B.J. Everitt, Essential Reproduction. 2nd ed. Blackwell Scientific Publications.London, 1984.
[25] J.C.L. Tou, R.E. Grindeland, and C.E. Wade. Effect of Diet and Exposure to Hindlimb Suspension on Estrous Cycling in Sprague-Dawley Rats. Am.J.Endrocrinol.Metab, 2003, 286.
[26 ] Ismudiono, P. Srianto, H. Anwar, S.P. Madyawati, A. Samik, dan E. Safitri, Buku Ajar Fisiologi Reproduksi pada Ternak. Airlangga University Press.Surabaya, 2010.
[27] C.D. Turner, dan J.T. Bagnara, Endokrinologi Umum. Edisi ke-6. Airlangga University Press. Surabaya, 1998, 564-607.
[28] P.P. Putro, Transfer Embrio pada Sapi. Fakultas Kedokteran Hewan, Universitas Gajah Mada. Yogyakarta, 2001.
[29] B.R. Lackey, S.L.L. Gray, and D.M. Henricks, Physiological Basis for Use of Insulin-Like Growth Factor in Reproductive Applications: A Review. Theriogenology 53 (2000) 1147-1156.
[30] R.I. Derar, S. Haramaki, M.D.S. Hoque, T. Hashizume, T. Osawa, K. Taya, G. Watanabe, and Y.I. Miyake, 2005. Insulin-Like Growth Factor-I as A Follicular Growth Promoter during Early Pregnancy in Thoroughbred Mares. Vet. Med. Sci., 2005.
[31] T.C. Lin, J.M. Yen, K.B. Gong, T.T. Hsu, and L.R. Chen, IGF-I/IGFBP-I Increases Blastocyst Formation and Total Blastocyst Cell Number in Mouse Embryo Culture and Facilitates The Establishment of AStem-Cell Line. BMC Cell Biology 4 (2003) 14-19.
[32] M.A. Velazquez, L.J. Spicer, and D.C. Wathes, The Role of Endocrine Insulin-Like Growth Factor-I (IGF-I) in Female Bovine Reproduction. Domestic Anim.Endocrinol.35 (2008) 325-342.
[33] J.V. Yelich, R.P. Wettemann, T.T. Marston, and L.J. Spicer, Luteinizing Hormone, Growth Hormone, Insulin-Like Growth Factor-I, Insulin and Metabolites before Puberty in Heifers Fed to Gain at Two Rates. Domest.Anim.Endocrinol 13 (1996) 325-38.