Characterization of Irradiation Induced Mutants of Cassava (Manihot esculenta Crantz) Generated from Jame-jame and Adira-4 Genotypes at M1V2 Generation

Abstract

Cassava (Manihot esculenta Crantz., Euphorbiaceae) is an important dietary carbohydrate source for approximately 800 million people in the tropics. It is a potential crop for food, feed, and industry. Cassava breeding through conventional approaches are hampered with some limitations which resulted in a low number of superior varieties. The objective of this research was to generate several mutant lines with higher yield and starch content. This research intended to identify the second generation (M1V2) Jame-jame and Adira-4 variant cassava mutants from gamma ray irradiation. Jame-jame is a local genotype from Halmahera (local high yielding genotype, sweet, and potential)  and Adira-4 is an Indonesia national variety of cassava. Mutant diversity appears on the plant height, plant branching, and plant tubers that harvested at 10 months after planting (MAP). Our results showed the positive correlation between plant height, first branching height, tuber weight, number of roots, and number of economic roots. Based on tuber weight and number of economic tuber there were 9 potential mutants (putative).  Based on the stability test, there were 6 potential stable mutants (putative) by first branching height, tuber weight, total number of tubers, and total number of economies tuber, namely: V5D1-(2), V5D1-2(2), V5D1-3(2), V5D1-4(3), V5D2-2(2), and V5D2-6(2).

 

Keywords: local genotype, M1V2, storage root, characters,  stability.

References
[1] Liu J., Zheng Q., Ma Q., Gadidasu K.K., Zhang P. 2011 Cassava genetic transformation and its application in breeding. J. Integr. Plant Biol. 53:552-569.

[2] FAO. 2010. FAOSTAT. (http://faostat.fao.org/).

[3] Stapleton G. 2012. Global starch market outlook and competing starch raw materials for starches by product segment and region. Cassava Starch World. 2012. Centre for Management Technology (CMT), Phnom Penh.

[4] Norton R.2014 Global starch market outlook and feedstock economics. Cassava World. Africa 2014. Centre for Management Technology (CMT), Lusaka.

[5] Ceballos, H, Kawuki, RS, Gracen, VE, Yencho, GC, and Hershey, CH. 2015. Conventional breeding, marker-assisted selection, genomic selection and inbreeding in clonally propagated crops: a case study for cassava. Theor Appl Genet. 128:1647– 1667

[6] Khumaida N, Ardie SW, Dianasari M, and Syukur M. 2015a. Cassava (Manihot esculenta Crantz.) Improvement through Gamma Irradiation. Procedia Food Science 3:27–34.

[7] Khumaida N, Maharani S, Ardie SW. 2015b. The leaf color performance on several lines of cassava and its relation with tuber yield as early reference. Procedia Environmental Sciences. Vol 24: 39-46.

[8] Oyeyemi SM, Lawal AO. 2010. Reduction of cyanide content in cassava by gamma irradiation from CirusCobol (60) teletherapy machine. J Appl Sci. 5: 69–73

[9] Tofino A, Cabal D, Sanchez T, Ceballos H. 2011. Identification of radiation induced mutants of cassava (Manihot esculenta Crantz.) using morphological and physicochemical descriptors. Agronomia Colombiana 29:179-188.