Probiotic Potential and Functional Properties of Lactobacillus Reuteri, Lactobacillus Rhamnosus and Lactobacillus Helveticus: A Comparative Study


This study was conducted to evaluate and comparethe probiotic propertiesofLactobacillus helveticusNK1, Lactobacillus rhamnosusF and Lactobacillus reuteriLR1lactobacilli strains.Changes in pH, cell growth, proteolytic activity, antioxidantactivity, and angiotensin-converting enzyme(ACE)inhibitoryactivity were monitored during fermentation ofreconstituted skim milk (RSM) by pure cultures of lactobacilli.Among the tested strains, L. helveticusNK1 showed the highest proteolytic, ACE inhibitoryand antioxidantactivitiesduring milk fermentation,followed by L. rhamnosus F and L. reuteriLR1.The promising capability of all of the lactobacilli strains to release bioactivepeptides from the milk proteins was demonstrated.

Keywords: Lactobacillus, probiotic, milk fermentation, bioactive peptides

[1] GobbettiM, Minervini F, Rizzello CG. Angiotensin I-converting-enzyme-inhibitory and antimicrobial bioactive peptides. International Journal of Dairy Technology. 2004;57(2–3):173–188.

[2] Griffiths MW, Tellez AM. Lactobacillus helveticus: The proteolytic system. Frontiers in Microbiology. 2013;4. 30-31 doi:10.3389/fmicb.2013.00030

[3] Solieri L, De Vero L, Tagliazucchi D. Peptidomic study of casein proteolysis in bovine milk by Lactobacillus casei PRA205 and Lactobacillus rhamnosus PRA331. International Dairy Journal. 2018;85:237–246.

[4] Fedorova TV, Vasina DV, Begunova AV, Rozhkova IV, Raskoshnaya TA, Gabrielyan NI. Antagonistic activity of lactic acid bacteria Lactobacillus spp. against clinical isolates of Klebsiella pneumoniae. Applied Biochemistry and Microbiology. 2018;54(3):277– 287.

[5] Begunova AV, Rozhkova IV, Zvereva EA, Glazunova OA, Fedorova TV. Lactic andpropionic acid bacteria: The formation of a community for the production of functional products with bifidogenic and hypotensitive properties. Applied Biochemistry and Microbiology. 2019;55(6):660–669.

[6] Torkova AA, Ryazantseva KA, Agarkova EY, Kruchinin AG, Tsentalovich MY, Fedorova TV. Rational design of enzyme compositions for the production of functional hydrolysates of cow milk whey proteins. Applied Biochemistry and Microbiology. 2017;53(6):669–679.

[7] Manguy J. Jehl P, Dillon ET, Davey NE, Shields DC, Holton TA. Peptigram: A webbased application for peptidomics data visualization. Journal of Proteome Research. 2017;16(2):712–719.

[8] Raak N, Rohm H, Jaros D. Enzymatic cross-linking of casein facilitates gel structure weakening induced by overacidification. Food Biophysics.2017;12(2):261–268.

[9] Pihlanto A, Virtanen T, Korhonen H. Angiotensin I converting enzyme (ACE) inhibitoryactivity and antihypertensive effect of fermented milk. International Dairy Journal. 2010;20(1):3–10.

[10] Leclerc P.-L., Gauthier SF, Bachelard H, Santure M, Roy D.Antihypertensive activity of casein-enriched milk fermented by Lactobacillus helveticus. International Dairy Journal. 2002;12(12):995–1004.

[11] Sadat-Mekmene L, Genay M, Atlan D, Lortal S, Gagnaire V. Original features of cellenvelope proteinases of Lactobacillus helveticus. A review. International Journal of Food Microbiology. 2011;146(1):1–13.

[12] Gobbetti M, Ferranti P, Smacchi E, Goffredi F, Addeo F. Production of angiotensini-converting-enzyme-inhibitory peptides in fermented milks started by Lactobacillus delbrueckiisubsp. bulgaricus SS1 and Lactococcus lactissubsp. cremoris FT4. Applied and Environmental Microbiology. 2000;66(9):3898–3904.

[13] Contreras M, Carrón R, Montero MJ, Ramos M, Recio I. Novel casein-derived peptides with antihypertensive activity. International Dairy Journal. 2009;19(10):566–573.

[14] Saito T, Nakamura T, Kitazawa H, Kawai Y, Itoh T. Isolation and structural analysis of antihypertensive peptides that exist naturally in gouda cheese. Journal of Dairy Science. 2000;83(7):1434–1440.

[15] Ali E, Nielsen SD, Abd-El Aal S, El-Leboudy A, Saleh E, LaPointe G. Use of mass spectrometry to profile peptides in whey protein isolate medium fermented by Lactobacillus helveticusLH-2 and Lactobacillus acidophilusLa-5. Frontiers in Nutrition. 2019;6. 152-153

[16] Abdel-Hamid M, Romeih E, GambaRR, Nagai E, Suzuki T, Koyanagi T, Enomoto T. The biological activity of fermented milk produced by Lactobacillus casei ATCC 393 during cold storage. International Dairy Journal. 2019;91:1–8.

[17] Maeno M, Yamamoto N, Takano T. Identification of an antihypertensive peptide from casein hydrolysate produced by a proteinase from Lactobacillus helveticus CP790. Journal of Dairy Science. 1996;79(8):1316–1321.

[18] Hayes M, Stanton C, Slattery H et al. Casein fermentate of Lactobacillus animalis DPC6134 contains a range of novel propeptide angiotensin-converting enzyme inhibitors. Applied and Environmental Microbiology. 2007;73(14):4658–4667