The Functional Properties of Bread Enriched with Essential Fatty Acids

Abstract

We developed ω-3-enriched bread by adding a liposomal polyunsaturated fatty acids (PUFAs) concentrate to the bread recipe. We determined that subsequent feeding of the ω-3-enriched bread to experimental animals in the alimentary dyslipidaemia state led to normalisation of the lipid profile of the blood serum, with a decrease in the total cholesterol, triglycerides, and low-density and very lowdensity lipoproteins. The high-density lipoproteins, antioxidants, reduced glutathione and glutathione reductase activity index increased compared to the corresponding indicators in animals with alimentary dyslipidaemia that were fed bread without ω-3. The ω-3-enriched bread diet significantly decreased harmful oxidation products (diene conjugates and malondialdehyde) in the blood plasma, erythrocytes and liver. Therefore, the results suggested that bread enriched with ω-3 fatty acids is a functional food with hypolipidaemic action. The results on the total content of fatty acids in lipids from bread samples prepared according to a standard recipe and bread enriched with concentrate showed that the relative content of omega-3 PUFAs in the fortified bread significantly increased by 3.2 times compared to bread without the addition of concentrate. The additive did not change the consumer qualities of the finished product (taste and smell of the bread).


Keywords: alimentary dyslipidaemia, antioxidant effect, bread, functional food, lipid profile, ω-3 polyunsaturated fatty acids

References
[1] Akbarzadeh A, Rezaei-Sadabady R, Davaran S et al. Liposome: Classification, preparation, and applications. Nanoscale Research Letters. 2013;8:9-11

[2] Averina ES, Kutyrev IA. Perspectives on the use of marine and freshwater hydrobiont oils for development of drug delivery systems. Biotechnology Advances. 2011;29:548–557.

[3] Beutler E, editor. Red cell metabolism, a manual of biochemical methods. New York: Grune and Straton; 1975.

[4] Bligh EG, Dyer WJ. A rapid method for total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 1959;37:911?917.

[5] Buttriss J. Are health claims and functional foods a route to improving the nation’s health? Food and Nutrition Bulletin. 2010;35:87?91.

[6] Cardoso SM, Pereira OR, Seca AML, Pinto DCGA, Silva AMS. Seaweeds as preventive agents for cardiovascular diseases: From nutrients to functional foods. Marine Drugs. 2015;13:6838?6865.

[7] Chaudhry Q, Scotter M, Blackburn J et al. Applications and implications of nanotechnologies for the food sector. Food Additives and Contaminants Part A Chem Anal Control Expo Risk Assess. 2008;25:241?258. doi: 10.1080/02652030701744538.

[8] Chowdhury R, Warnakula S, Kunutsor S et al. Association of dietary, circulating, and supplement fatty acids with coronary risk: A systematic review and meta-analysis. Annals of Internal Medicine. 2014;160:398–406. doi:10. 7326/m13-1788.

[9] de Assis AM, Rech A, Longoni A et al. Omega-3 polyunsaturated fatty acids prevent lipoperoxidation, modulate antioxidant enzymes, and reduce lipid content but do not alter glycogen metabolism in the livers of diabetic rats fed on a high fat thermolyzed diet. Molecular and Cellular Biochemistry. 2012;361:151–160. doi: 10.1007/s11010-011- 1099-4.

[10] de Conto LC, Oliveira RSP, Martin LGP, Chang YK, Steel CJ. Effects of the addition of microencapsulated omega-3 and rosemary extract on the technological and sensory quality of white pan bread. LWT - Food Science and Technology. 2012;45:103?109. doi: 10.1016/j.lwt.2011.07.027.

[11] Ernster L, Nordenbrand K. Microsomal lipid peroxidation. Methods in Enzymology. 1967;10:574?580.

[12] Ganesan B, Brothersen C, McMahon DJ. Fortification of foods with omega3 polyunsaturated fatty acids. Critical Reviews in Food Science and Nutrition 2014;54:98?114. doi:10.1080/10408398.2011.578221.

[13] Garg ML, Wood LG, Singh H, Moughan PJ. Means of delivering recommended levels of long chain n-3 polyunsaturated fatty acids in human diets. Journal of Food Science2006;71:66?71.

[14] Gavrilov VB, Mishkorudnaya MI. Spektrofotometricheskoye opredeleniye soderzhaniya gidroperekisey lipidov v plazme krovi [Spectrophotometric determination of lipid hydroperoxides in blood plasma]. Lab Delo. 1983;3:33?36.

[15] Giordano E, Visioli F. Long-chain omega 3 fatty acids: Molecular bases of potential antioxidant actions. Prostaglandins Leukot Essent Fatty Acids. 2014;90:1–4. http://dx.doi.org/10.1016/j.plefa.2013.11.002.

[16] Glatzle D, Vuilleumier JP, Weber F, Decker K. Glutathione reductase test with whole blood, a convenient procedure for the assessment of riboflavin status in humans. Cellular and Molecular Life Sciences. 1974;30:665?667.

[17] Gómez-Candela C, Bermejo-López LM, Loria-Kohen V. Importance of a balanced omega 6/omega 3 ratio for the maintenance of health. Nutritional recommendations. Nutrición Hospitalaria2011;26:323?329.

[18] Grahl-Nielsen O, Halvorsen A-K, Bodoev N et al. Fatty acid composition of blubber of the Baikal seal Phoca sibirica and its marine relative, the ringed seal P. hispidaMarine Ecology Progress Series. 2005;305:261–274.

[19] James MJ, Gibson RA, Cleland LG. Dietary polyunsaturated fatty acids and inflammatory mediator production. The American Journal of Clinical Nutrition. 2000;71:343–348.

[20] Kaushik P, Dowling K, Barrow CJ, Adhikari B. Microencapsulation of omega-3 fatty acids: A review of microencapsulation and characterization methods. Journal of Functional Foods. 2015;19:868?881. doi: 10.1016/j.jff.2014.06.029.

[21] Kravchenko LV, Aksenov IV, Avren’eva LI, Beketova NA, Trusov NV, Guseva GV. Vliyaniye polinenasyshchennykh zhirnykh kislot omega-3 na nekotoryye pokazateli antioksidantnogo potentsiala krys [The influence of omega-3 polyunsaturated fatty acids on some indicators of the antioxidant capacity of rats]. Voprosy Pitaniya. 2013;82:4?9.

[22] Krinsky NI. Membrane antioxidants. Annals of the New York Academy of Sciences. 1988;551:17?32.

[23] Kulinskij VI, Kolesnichenko LS. Sistema glutationa I. Sintez, transport glutationtransferazy, glutationperoksidazy [The glutathione system. I. Synthesis, transport, glutathione transferases, glutathione peroxidases]. Biomedicinskaya Khimiya. 2009;55:255?277.

[24] Lamazhapova GP, Syngeeva EV, Kozlova ?S, Zhamsaranova SD. Razrabotka liposomal’noy formy kontsentrata polinenasyshchennykh zhirnykh kislot: vozmozhnyye puti ispol’zovaniya pri proizvodstve funktsional’nykh pishchevykh produktov [Development of a liposomal form of a concentrate of polyunsaturated fatty acids: possible ways of using in the production of functional food products]. Voprosy Pitaniya. 2017;86:80-88.

[25] Metodicheskiye rekomendatsii MP 2.3.1.2432-08. Normy fiziologicheskikh potrebnostey v energii i pishchevykh veshchestvakh dlya razlichnykh grupp naseleniya Rossiyskoy Federatsii (utv. Glavnym gosudarstvennym sanitarnym vrachom RF 18 dekabrya 2008) [Methodical recommendations MP 2.3.1.2432-08. Norms of physiological needs for energy and nutrients for different population groups of the Russian Federation (approved by the Chief State Sanitary Doctor of the Russian Federation on December 18, 2008]. Ekologicheskiye Vedomosti. 2009;March;3:6?42.

[26] Mihara M, Uchiyama M, Fukuzawa K. Thiobarbituric acid value on fresh homogenate of rat as a parameter of lipid peroxidation in aging, CCl4 intoxication, ?nd vitamin E deficiency. Biochemia Medica1980;23:302?311.

[27] Placer Z. Lipoperoxydationssysteme im biologischen Material 2. Bestimmung der Lipoperoxydation im Säugetierorganismus [Lipoperoxidation systems in biological material 2. Determination of lipoperoxidation in the mammalian organism]. Nahrung. 1968;12:679?684.

[28] Riediger ND, Othman RA, Suh M, Moghadasian MH. A systemic review of the roles of n-3 fatty acids in health and disease. Journal of the American Dietetic Association. 2009;109:668?679.

[29] Ruiz JCR, Vazquez EDLLO, Campos MRS. Encapsulation of vegetable oils as source of omega-3 fatty acids for enriched functional foods. Critical Reviews in Food Science and Nutrition2017;57:1423?1434. doi: 10.1080/10408398.2014.1002906.

[30] Rustan AC, Drevon CA. Fatty acids: Structures and properties. Encyclopedia of Life Sciences. 2005: 1. 1-7. doi: 10.1038/npg.els.0003894

[31] Sanguansri L, Day L, Shen Z et al. Encapsulation of mixtures of tuna oil, tributyrin and resveratrol in a spray dried powder formulation. Food and Function Journal 2013;4:1794?1802. doi:10.1039/C3FO60197H.

[32] Bresson, Jean-Louis & Flynn, Albert & Heinonen, Marina et al. Scientific opinion of the panel on dietetic products, nutrition and allergies on a request from European Commission related to labeling reference intake values for n-3 and n-6 polyunsaturated fatty acids. European Food Safety Agency Journal. 2009;1176:1?11.

[33] Taylor TM, Weiss J, Davidson PM, Bruce BD. Liposomal nanocapsules in food science and agriculture. Critical Reviews in Food Science and Nutrition. 2005;45:587?605.

[34] Visioli F, Galli C. Evaluating oxidation processes in relation to cardiovascular disease: a current review of oxidant/antioxidant methodology Nutrition Metabolism and Cardiovascular Diseases. 1997;7:459–466.

[35] Vladimirov Y?, Archakov AI. Perekisnoye okisleniye lipidov v biologicheskikh membranakh [Lipid peroxidation in biological membranes]. Moscow: Nauka; 1972.

[36] World Health Organization. Global health risks: Mortality and burden of disease attributable to selected major risks. Geneva: World Health Organization; 2009.

[37] Zhamsaranova SD, Lamazhapova GP, Syngeeva EV. Development of a method to produce a concentrate of polyunsaturated fatty acids. BIOSCIENCES BIOTECHNOLOGY RESEARCH ASIA. 2014;11:59?64.

[38] Zhamsaranova SD, Lamazhapova GP, Syngeeva EV, Burnasheva EV. The effect of the concentrate of polyunsaturated fatty acids on indicators of oxidative stress in experimental dyslipidemia. Bulletin of experimental biology and medicine. 2015;7:106-15.