The Influence of Fucoxanthin Immobilized on Porous Aluminum-Silicon Carrier Surface on the Functional Activities of Immunocytes in Mice


Fucoxanthin is a natural carotenoid obtained from seaweed which exhibits antioxidant properties. This research aimed to assess whether fucoxanthin, immobilized on aluminum-silicon carrier particles, has a toxic effect on immune cells. The viability, proliferation, nitric oxide production and myeloperoxidase activity of thymocytes and splenocytes of mice in vitro were studied. It was shown that fucoxanthin, immobilized on aluminum-silicon carrier particles, increased the survival rate and proliferation of mature immunocytes (splenocytes) after 24 hours exposure and increased the survival rate of naïve immunocytes (thymocytes) when exposed for 120 hours. In terms of myeloperoxidase, the activity of the immune cells was not affected by fucoxanthin immobilized on the carrier particles. The obtained results indicated that fucoxanthin, immobilized on particles of an aluminum-silicon carrier, did not have a toxic effect on mouse immunocytes.

Keywords: Cylindrotheca closterium, fucoxanthin, γ-aluminum oxide, polydimethylsiloxane, thymocytes, splenocytes, viability, proliferation, nitric oxide, myeloperoxidase activity

[1] Zhu Y, Cheng J, Min Z et al. Effects of fucoxanthin on autophagy and apoptosis in SGC-7901 cells and the mechanism. Journal of Cellular Biochemistry 2018;119(9):7274-7284.

[2] Zeng J, Zhang Y, Ruan J et al. Protective effects of fucoxanthin and fucoxanthinol against tributyltin-induced oxidative stress in HepG2 cells. Environmental Science and Pollution Research. 2018;25(6);5582-5589.

[3] Moghadamtousi ZS, Karimian H, Khanabdali R et al. Anticancer and antitumor potential of fucoidan and fucoxanthin, two main metabolites isolated from brown algae. The Scientific World Journal. 2014. 2:768323-768324

[4] Molina N, Morandi AC, Bolin AP, Otton R. Comparative effect of fucoxanthin and vitamin C on oxidative and functional parameters of human lymphocytes. International Immunopharmacology. 2014;22(1):41-50.

[5] Lykov A, Surovtseva M, Rachkovskaya L et al. Single wall carbone nanotubes functionalized with composition of γ-aluminum oxide and polydimethylsiloxane properties. IEEE International Multi-Conference on Engineering, Computer and Information Sciences (SIBIRCON); October 21st 2019, Novosibirsk, Russia.

[6] Dastjeri R, Bahrizadeg S. Polysiloxane-shield nano-patterned nanolayers: Multicomponent reactors for photocatalytic purification. Biointerface Research in Applied Chemistry. 2018;8(1):3105-3110.

[7] Taira J, Sonamoto M, Uehara M. Dual biological functions of a cytoprotective effect and appotosis induction by bioavailable marine carotenoid fucoxanthinol through modulation of the Nrf2 ctivation in RAW264.7 Macrophage Cells. Marine Drugs. 2017;15:304-318.

[8] Iio K, Okada Y, Ishikura M. Single and 13-week oral toxicity study of fucoxanthin oil from microalgae in rats. Shokuhin Eiseigaku Zasshi. 2011;52(3):183-189.

[9] Kawashima T. A marine carotenoid, fucoxanthin, induces regulatory T cells and inhibits Th17 cell differentiation in vitro. Bioscience Biotechnology and Biochemistry. 2011;75(10):2066-2069.

[10] Kong ZL, Sudirman S, Hsu YC et al. Fucoxanthin-rich brown algae extract improves male reproductive function on streptozotocin-nicotinamide-induced diabetic rat model. International Journal of Molecular Sciences. 2019. 20(18):4485-4486