The Growth Kinetics of RhizoctoniasolaniAfter 1 MeV Electron Irradiation

Abstract

Crops, especially potatoes, are prone to a wide range of fungal, viral and bacterial diseases, including black scurf caused by Rhizoctoniasolani. This study focused on the radiation treatment of the phytopathogenic fungus RhizoctoniasolaniKuhn, grown from sclerotium irradiated with 1 MeV electrons in the dose range from 20 to 4500 Gy. The doses absorbed by the sclerotia were determined using computer simulation. The growth of the fungus samples was monitored after 24, 48, 72, and 96 hours from the time of seeding. It was found that the dependence of the radial growth velocity of R. solani on the time after irradiation with doses ranging from 20to 1800 Gywas nonlinear. Irradiation at a dose over 4500 Gyled to complete suppression of the germination of R. solani sclerotia.


Keywords: radiation treatment, electron radiation, radiation dose, sclerotia of Rhizoctoniasolani, Kuhn, radial velocity of growth

References
[1] UlyanenkoLN, OudalovaAA. Environmental health assessment based on agricultural plants responses to ionizing radiation. RadiationandRisk. 2015;24(1): 118-131.

[2] Feliciano CP, De Guzman ZM, Tolentino LM, Cobar ML, Abrera GB Radiation-treated ready-to-eat (RTE) chicken breast adobofor immuno-compromised patients.Food Chemistry. 2014;163:142-146.

[3] Blessington T., Scheuring D., Nzaramba M. et al. The use of low-dose electronbeam irradiation and storageconditions for sprout control and their effects on xanthophylls, antioxidant capacity, and phenolics in the potato cultivaratlantic.American Journal of Potato Research. 2015;92:609-618.

[4] Nam HA, Ramakrishnan SR, Kwon JH Effects of electron-beam irradiation on the quality characteristics ofmandarin oranges (Citrus unshiu (Swingle) Marcov) during storage. Food Chemistry. 2019;286:338-345.

[5] Kim J, Moreira RG, Castell-Perez ME. Validation of irradiation of broccoli with a 10 MeV electronbeam accelerator.Journal of Food Engineering. 2008;86:595-603.

[6] Cárcel J., Benedito J., Cambero I., Cabeza M., Ordónez J. Modeling and optimization of the E-beamtreatment of chicken steaks and hamburgers,considering food safety, shelf-life, and sensoryquality. Food and Bioproducts Processing. 2015;96:133-144.

[7] Chernyaev AP, Avdyuhina VM, Bliznuk UA et al. The impact of X-ray radiation on sprouting and protein and sugar content in potato tubers. Technologies of Living Systems. 2019;16(1):59-66.

[8] Loy NN, Gubareva OS, Sanzharova NI, Gulina SN, Shchagina NI, Mironova MP The ionizing radiation effect on the vital capacity of pests and quality of grain and grain products. Vestnik of the Russian Agricultural Science.2016;6:53-55.

[9] Evstratova LP, Nikolaeva EV, Kuznetsova LA, Harin VN, Spektor EN Potato damage by soil pathogens in the conditions of Karelia. Agro XXI. 2006;4-6:10-12.

[10] Khalikov SS,Malyuga AA, Chulikova NS.Ecological safe preparations based on mechanochemical modification of tebuconazole for complex protection of potatoes. Agrohimia. 2018;10:46-53.

[11] Ivanyuk VG, Aleksandrov OT. Efficiency of agrotechnical measures against potato rhizoctoniosis. News of the Academy of Agrarian Sciences of the Republic of Belarus. 1996;2:55-60.

[12] Malyuga AA, Enina NN, Shcheglova OV, Chulikova NS The precursors’ role in the control of Rhizoctoniasolani. Zashita I Karantin Rasteniy. 2011;1:28-30.

[13] Dudka IA, Vasser SP, Ellanskaya IA et al. Methods of experimental mycology: Reference.Bilay VI, editor. Kiev: Naukova Dumka; 1982.

[14] PanikovNS. Kinetics of the growth of microorganisms: General laws and ecological applications. Moscow: Nauka; 1991.