Smartphone-based Wireless Operated Milliliter-scale Bioreactor

Abstract

This paper presents the development of a smartphone-based wireless operated milliliter scale bioreactor. The bioreactor has a working volume of 18 mL and is made of poly (methylmethacrylate) (PMMA) and poly (dimethylsiloxane) (PDMS) polymers using a laser engraving methodology. Temperature and reactor stirring speed are the controlled variables and wireless communication is achieved through a wireless transceiver embedded in a low-cost controller platform. The results attained are very promising as the controller is able to control the desired reactor variables precisely through wireless communications.

References
[1] C. Lattermann, and J. Buchs, Microscale and miniscale fermentation and screening, Current Opinion in Biotechnology, 35, 1–6, (2015), 10.1016/j.copbio.2014.12.005.


[2] A. R. Husain, Y. Hadad, and M. N. H. Zainal Alam, Development of Low-Cost Microcontroller-Based Interface for Data Acquisition and Control of Microbioreactor Operation, Journal of Laboratory Automation, (2015), 10.1177/2211068215594770, In press.


[3] M. M. Maharbiz, W. J. Holtz, R. T. Howe, et al., Microbioreactor arrays with parametric control for high-throughput experimentation, BiotechnolBioeng, 85, 376–381, (2004).


[4] N. Szita, P. Bocazzi, Z. Zhang, et al., Development of a multiplexed microbioreactor system for highthroughput bioprocessing, Lab Chip, 5, 819–826, (2005), 10.1039/b504243g.


[5] P. Harms, Y. Kostov, J. A. French, et al., Design and Performance of a 24-Station High Throughput Microbioreactor, BiotechnolBioeng, 93, no. 1, 6–13, (2006).


[6] Antti Vasala, Johanna Panula, Monika Bollók, Lutz Illmann, Christian Hälsig, and Peter Neubauer, A new wireless system for decentralised measurement of physiological parameters from shake flasks, Microbial Cell Factories, 5, no. 8, (2006), 10.1186/1475-2859-5-8.


[7] Eric Moreau, Floyd Inman, Sunita Singh, Heather Walters, and Leonard Holmes, Remote Control of Fed-Batch Fermentation Systems, J. Chem. Chem. Eng., 5, 897–902, (2011).