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., F., Ulwiyyah, N. H., Saidi, N., & Iqbalsyah, T. M. (2019). Screening and Production of Lipase from a Thermo-halophilic Bacterial Isolate of Pria Laot Sabang 80 Isolated from Under Water Hot Spring. KnE Engineering, 4(2), 105–115. https://doi.org/10.18502/keg.v1i2.4436

https://doi.org/10.18502/keg.v1i2.4436

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authors

  • Febriani .
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  • Nurul H. Ulwiyyah
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  • Nurdin Saidi
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  • Teuku M. Iqbalsyah
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Published Date

  • Apr 16, 2019

Screening and Production of Lipase from a Thermo-halophilic Bacterial Isolate of Pria Laot Sabang 80 Isolated from Under Water Hot Spring

KnE Engineering / International Conference on Basic Sciences and Its Applications (ICBSA-2018) / Pages 105–115

Abstract

Lipase is an important class of industrial hydrolytic enzyme that is produced mainly by bacteria. The goal of this study was to screen and determine the optimum conditions for lipase production by a thermo-halophilic bacterium, named Pria Laot Sabang 80 (PLS 80), isolated from an underwater hot spring. Screening of lipase was conducted on agar media supplemented with olive oil and rodhamine B. PLS 80 exhibited lipase activity based on the formation of orange red fluorescence area around the colonies observed under UV light. The growth curve and lipase activity of the isolate was studied at various temperature, incubation time, pH and salt concentrations. The growth curve was measured by dry cell biomass and lipase activity using spectrophotometry method by p-nitrophenyl palmitate as the substrate. The isolate showed the highest biomass production after 16 hours of fermentation at 70 ∘C, pH 9 and with an addition of 3 M NaCl. The highest activity of lipase was 24.3 U/mL. This study shows that PLS 80 produced a thermo-halostable alkaline lipase.


 


 


Keywords: Thermostable, halostable, lipase, Pria Laot Sabang

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